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Involvement of Mitochondria in the Selective Response to Microsecond Pulsed Electric Fields on Healthy and Cancer Stem Cells in the Brain.

التفاصيل البيبلوغرافية
العنوان: Involvement of Mitochondria in the Selective Response to Microsecond Pulsed Electric Fields on Healthy and Cancer Stem Cells in the Brain.
المؤلفون: Casciati A; Division of Health Protection Technologies, Italian National Agency for Energy New Technologies and Sustainable Economic Development (ENEA), Via Anguillarese 301, 00123 Rome, Italy., Taddei AR; Great Equipment Center-Section of Electron Microscopy, University of Tuscia, Largo dell'Università snc, 01100 Viterbo, Italy., Rampazzo E; Department of Women's and Children's Health (SDB), University of Padova, Via Giustiniani 3, 35128 Padova, Italy.; Pediatric Research Institute (IRP), Corso Stati Uniti 4, 35127 Padova, Italy., Persano L; Department of Women's and Children's Health (SDB), University of Padova, Via Giustiniani 3, 35128 Padova, Italy.; Pediatric Research Institute (IRP), Corso Stati Uniti 4, 35127 Padova, Italy., Viola G; Department of Women's and Children's Health (SDB), University of Padova, Via Giustiniani 3, 35128 Padova, Italy.; Pediatric Research Institute (IRP), Corso Stati Uniti 4, 35127 Padova, Italy., Cani A; Department of Women's and Children's Health (SDB), University of Padova, Via Giustiniani 3, 35128 Padova, Italy.; Pediatric Research Institute (IRP), Corso Stati Uniti 4, 35127 Padova, Italy., Bresolin S; Department of Women's and Children's Health (SDB), University of Padova, Via Giustiniani 3, 35128 Padova, Italy.; Pediatric Research Institute (IRP), Corso Stati Uniti 4, 35127 Padova, Italy., Cesi V; Division of Health Protection Technologies, Italian National Agency for Energy New Technologies and Sustainable Economic Development (ENEA), Via Anguillarese 301, 00123 Rome, Italy., Antonelli F; Division of Health Protection Technologies, Italian National Agency for Energy New Technologies and Sustainable Economic Development (ENEA), Via Anguillarese 301, 00123 Rome, Italy., Mancuso M; Division of Health Protection Technologies, Italian National Agency for Energy New Technologies and Sustainable Economic Development (ENEA), Via Anguillarese 301, 00123 Rome, Italy., Merla C; Division of Health Protection Technologies, Italian National Agency for Energy New Technologies and Sustainable Economic Development (ENEA), Via Anguillarese 301, 00123 Rome, Italy., Tanori M; Division of Health Protection Technologies, Italian National Agency for Energy New Technologies and Sustainable Economic Development (ENEA), Via Anguillarese 301, 00123 Rome, Italy.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Feb 13; Vol. 25 (4). Date of Electronic Publication: 2024 Feb 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Mitochondria*/metabolism , Neoplasms*/metabolism, Cell Membrane/metabolism ; Electricity ; Cytoskeleton/metabolism ; Brain/metabolism
مستخلص: In the last few years, pulsed electric fields have emerged as promising clinical tools for tumor treatments. This study highlights the distinct impact of a specific pulsed electric field protocol, PEF-5 (0.3 MV/m, 40 μs, 5 pulses), on astrocytes (NHA) and medulloblastoma (D283) and glioblastoma (U87 NS) cancer stem-like cells (CSCs). We pursued this goal by performing ultrastructural analyses corroborated by molecular/omics approaches to understand the vulnerability or resistance mechanisms triggered by PEF-5 exposure in the different cell types. Electron microscopic analyses showed that, independently of exposed cells, the main targets of PEF-5 were the cell membrane and the cytoskeleton, causing membrane filopodium-like protrusion disappearance on the cell surface, here observed for the first time, accompanied by rapid cell swelling. PEF-5 induced different modifications in cell mitochondria. A complete mitochondrial dysfunction was demonstrated in D283, while a mild or negligible perturbation was observed in mitochondria of U87 NS cells and NHAs, respectively, not sufficient to impair their cell functions. Altogether, these results suggest the possibility of using PEF-based technology as a novel strategy to target selectively mitochondria of brain CSCs, preserving healthy cells.
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معلومات مُعتمدة: grant agreement no. 737164 SUMCASTEC European Union's Horizon 2020
فهرسة مساهمة: Keywords: CD133 protein; cancer stem cells (CSCs); filopodium-like protrusions; glioma; medulloblastoma; mitochondria dysfunction; normal human astrocyte; transcriptomics
تواريخ الأحداث: Date Created: 20240224 Date Completed: 20240226 Latest Revision: 20240227
رمز التحديث: 20240227
مُعرف محوري في PubMed: PMC10889160
DOI: 10.3390/ijms25042233
PMID: 38396911
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25042233