دورية أكاديمية
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Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues of Apocynum venetum .

التفاصيل البيبلوغرافية
العنوان: Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues of Apocynum venetum .
المؤلفون: Li X; Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin 150040, China., Zhang T; Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin 150040, China., Jiang L; State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China., Fan G; Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
المصدر: Genes [Genes (Basel)] 2024 Feb 11; Vol. 15 (2). Date of Electronic Publication: 2024 Feb 11.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Peptide Elongation Factor 1*/genetics , Apocynum*/genetics, Real-Time Polymerase Chain Reaction ; Reproducibility of Results ; Genes, Plant
مستخلص: Apocynum venetum L. is an economically valuable plant with tolerance to drought and salinity. Its leaves are utilized in tea production and pharmaceuticals, while the stem bark serves as a high-quality fiber material. To gain insights into the gene expression patterns of A. venetum using quantitative real-time PCR (qRT-PCR), it is crucial to identify appropriate reference genes. This study selected nine candidate genes, including α-tubulin ( TUA ), β-tubulin ( TUB ), actin ( ACT ), cyclophilin ( CYP ), elongation factor-1α ( EF-1α ), the B family of regulatory subunits of protein phosphatase ( PPP2R2 , PPP2R3 , and PPP2R5 ), and phosphoglycerate kinase ( PGK ), to determine the most appropriate reference genes in the leaf, stem, and root tissues of A. venetum . A comprehensive ranking by geNorm, NormFinder, BestKeeper, and RefFinder software and Venn diagrams was used to screen more stable reference genes in different tissues. The two most stable reference genes were CYP and TUA in leaves, PGK and PPP2R3 in stems, and TUA and EF-1α in roots, respectively. The relative expression values of the four genes involved in proline metabolism under polyethylene glycol treatment were used to validate the screened reference genes, and they exhibited highly stable expression levels. These findings represent the first set of stable reference genes for future gene expression studies in A. venetum . They significantly contribute to enhancing the accuracy and reliability of gene expression analyses in this economically important plant species.
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فهرسة مساهمة: Keywords: Apocynum venetum; gene expression; real-time PCR; reference genes; validation
المشرفين على المادة: 0 (Peptide Elongation Factor 1)
تواريخ الأحداث: Date Created: 20240224 Date Completed: 20240226 Latest Revision: 20240614
رمز التحديث: 20240615
مُعرف محوري في PubMed: PMC10888412
DOI: 10.3390/genes15020231
PMID: 38397220
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4425
DOI:10.3390/genes15020231