دورية أكاديمية
Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues of Apocynum venetum .
العنوان: | Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues of Apocynum venetum . |
---|---|
المؤلفون: | Li X; Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin 150040, China., Zhang T; Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin 150040, China., Jiang L; State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China., Fan G; Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin 150040, China. |
المصدر: | Genes [Genes (Basel)] 2024 Feb 11; Vol. 15 (2). Date of Electronic Publication: 2024 Feb 11. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel : MDPI |
مواضيع طبية MeSH: | Peptide Elongation Factor 1*/genetics , Apocynum*/genetics, Real-Time Polymerase Chain Reaction ; Reproducibility of Results ; Genes, Plant |
مستخلص: | Apocynum venetum L. is an economically valuable plant with tolerance to drought and salinity. Its leaves are utilized in tea production and pharmaceuticals, while the stem bark serves as a high-quality fiber material. To gain insights into the gene expression patterns of A. venetum using quantitative real-time PCR (qRT-PCR), it is crucial to identify appropriate reference genes. This study selected nine candidate genes, including α-tubulin ( TUA ), β-tubulin ( TUB ), actin ( ACT ), cyclophilin ( CYP ), elongation factor-1α ( EF-1α ), the B family of regulatory subunits of protein phosphatase ( PPP2R2 , PPP2R3 , and PPP2R5 ), and phosphoglycerate kinase ( PGK ), to determine the most appropriate reference genes in the leaf, stem, and root tissues of A. venetum . A comprehensive ranking by geNorm, NormFinder, BestKeeper, and RefFinder software and Venn diagrams was used to screen more stable reference genes in different tissues. The two most stable reference genes were CYP and TUA in leaves, PGK and PPP2R3 in stems, and TUA and EF-1α in roots, respectively. The relative expression values of the four genes involved in proline metabolism under polyethylene glycol treatment were used to validate the screened reference genes, and they exhibited highly stable expression levels. These findings represent the first set of stable reference genes for future gene expression studies in A. venetum . They significantly contribute to enhancing the accuracy and reliability of gene expression analyses in this economically important plant species. |
References: | J Ethnopharmacol. 2012 May 7;141(1):1-8. (PMID: 22421379) Nat Prod Res. 2014;28(12):928-31. (PMID: 24588258) BMC Mol Biol. 2010 Sep 20;11:73. (PMID: 20854682) PeerJ. 2023 Mar 7;11:e14966. (PMID: 36908824) Phytochemistry. 2019 Aug;164:1-11. (PMID: 31054374) J Physiol Biochem. 2018 May;74(2):301-312. (PMID: 29541948) J Plant Physiol. 2023 Feb;281:153925. (PMID: 36657231) Pharmacol Ther. 2019 Sep;201:181-201. (PMID: 31158394) Sci Rep. 2016 Dec 06;6:38513. (PMID: 27922100) Appl Biochem Biotechnol. 2014 Jul;173(6):1431-45. (PMID: 24811734) Data Brief. 2020 Nov 04;33:106494. (PMID: 33251304) Plant Physiol Biochem. 2021 Feb;159:268-276. (PMID: 33401201) Nat Methods. 2013 Oct;10(10):1003-5. (PMID: 23995387) Funct Integr Genomics. 2023 Apr 15;23(2):125. (PMID: 37060478) Methods. 2001 Dec;25(4):402-8. (PMID: 11846609) Sci Rep. 2019 Jun 3;9(1):8182. (PMID: 31160644) J Microbiol Methods. 2019 Aug;163:105658. (PMID: 31251967) Plants (Basel). 2023 Nov 23;12(23):. (PMID: 38068583) Sci Rep. 2015 Nov 19;5:16961. (PMID: 26581656) PLoS One. 2014 Mar 20;9(3):e92262. (PMID: 24651080) Plant Physiol Biochem. 2017 Jun;115:126-140. (PMID: 28364709) |
فهرسة مساهمة: | Keywords: Apocynum venetum; gene expression; real-time PCR; reference genes; validation |
المشرفين على المادة: | 0 (Peptide Elongation Factor 1) |
تواريخ الأحداث: | Date Created: 20240224 Date Completed: 20240226 Latest Revision: 20240614 |
رمز التحديث: | 20240615 |
مُعرف محوري في PubMed: | PMC10888412 |
DOI: | 10.3390/genes15020231 |
PMID: | 38397220 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2073-4425 |
---|---|
DOI: | 10.3390/genes15020231 |