دورية أكاديمية
Comprehensive Assessment of Inactivation Methods for Madariaga Virus.
| العنوان: | Comprehensive Assessment of Inactivation Methods for Madariaga Virus. |
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| المؤلفون: | Boytz R; Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06520, USA., Keita K; Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA., Pawlak JB; Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA., Laurent-Rolle M; Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06520, USA.; Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.; Center for Infection and Immunity, Yale University School of Medicine, New Haven, CT 06520, USA. |
| المصدر: | Viruses [Viruses] 2024 Jan 30; Vol. 16 (2). Date of Electronic Publication: 2024 Jan 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI |
| مواضيع طبية MeSH: | Alphavirus* , Encephalitis Virus, Eastern Equine*/physiology , Encephalomyelitis, Equine* , Phenols*, Horses ; Animals ; Guanidines ; Formaldehyde |
| مستخلص: | The Eastern Equine Encephalitis Virus (EEEV) is an emerging public health threat, with the number of reported cases in the US increasing in recent years. EEEV is a BSL3 pathogen, and the North American strain is a US Federal Select Agent (SA). These restrictions make experiments with EEEV difficult to perform, as high-tech equipment is often unavailable in BSL3 spaces and due to concerns about generating aerosols during manipulations. Therefore, a range of inactivation methods suitable for different downstream analysis methods are essential for advancing research on EEEV. We used heat, chemical, and ultraviolet (UV)-based methods for the inactivation of infected cells and supernatants infected with the non-select agent Madariaga virus (MADV). Although the MADV and EEEV strains are genetically distinct, differing by 8-11% at the amino acid level, they are expected to be similarly susceptible to various inactivation methods. We determined the following to be effective methods of inactivation: heat, TRIzol LS, 4% PFA, 10% formalin, and UV radiation for infected supernatants; TRIzol, 2.5% SDS with BME, 0.2% NP40, 4% PFA, and 10% formalin for infected cells. Our results have the potential to expand the types and complexity of experiments and analyses performed by EEEV researchers. |
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| معلومات مُعتمدة: | Emerging Pathogens Initiative United States HHMI Howard Hughes Medical Institute; 5TL1TR001864-08 United States TR NCATS NIH HHS; T32 AI055403 United States NH NIH HHS |
| فهرسة مساهمة: | Keywords: Eastern Equine Encephalitis Virus; Madariaga; Select Agent; inactivation; protocol |
| المشرفين على المادة: | 0 (trizol) 0 (Guanidines) 1HG84L3525 (Formaldehyde) 0 (Phenols) |
| تواريخ الأحداث: | Date Created: 20240224 Date Completed: 20240226 Latest Revision: 20240522 |
| رمز التحديث: | 20240523 |
| مُعرف محوري في PubMed: | PMC10892135 |
| DOI: | 10.3390/v16020206 |
| PMID: | 38399982 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1999-4915 |
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| DOI: | 10.3390/v16020206 |