دورية أكاديمية
Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke.
| العنوان: | Validation and application of caged Z-DEVD-aminoluciferin bioluminescence for assessment of apoptosis of wild type and TLR2-deficient mice after ischemic stroke. |
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| المؤلفون: | Josić Dominović P; University of Zagreb School of Medicine, BIMIS - Biomedical Research Center Šalata, and Croatian Institute for Brain Research, Zagreb, Croatia., Dobrivojević Radmilović M; University of Zagreb School of Medicine, BIMIS - Biomedical Research Center Šalata, and Croatian Institute for Brain Research, Zagreb, Croatia., Srakočić S; University of Zagreb School of Medicine, BIMIS - Biomedical Research Center Šalata, and Croatian Institute for Brain Research, Zagreb, Croatia., Mišerić I; University of Zagreb School of Medicine, BIMIS - Biomedical Research Center Šalata, and Croatian Institute for Brain Research, Zagreb, Croatia., Škokić S; University of Zagreb School of Medicine, BIMIS - Biomedical Research Center Šalata, and Croatian Institute for Brain Research, Zagreb, Croatia., Gajović S; University of Zagreb School of Medicine, BIMIS - Biomedical Research Center Šalata, and Croatian Institute for Brain Research, Zagreb, Croatia. Electronic address: srecko.gajovic@hiim.hr. |
| المصدر: | Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2024 Apr; Vol. 253, pp. 112871. Date of Electronic Publication: 2024 Feb 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Switzerland NLM ID: 8804966 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2682 (Electronic) Linking ISSN: 10111344 NLM ISO Abbreviation: J Photochem Photobiol B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Elsevier Sequoia, 1987- |
| مواضيع طبية MeSH: | Toll-Like Receptor 2*/genetics , Toll-Like Receptor 2*/metabolism , Ischemic Stroke* , Oligopeptides*, Firefly Luciferin/*analogs & derivatives, Mice ; Animals ; Caspase 3/metabolism ; Apoptosis |
| مستخلص: | Programmed cell death or apoptosis is a critically important mechanism of tissue remodeling and regulates conditions such as cancer, neurodegeneration or stroke. The aim of this research article was to assess the caged Z-DEVD-aminoluciferin substrate for in vivo monitoring of apoptosis after ischemic stroke in TLR2-deficient mice and their TLR2-expressing counterparts. Postischemic inflammation is a significant contributor to ischemic injury development and apoptosis, and it is modified by the TLR2 receptor. Caged Z-DEVD-aminoluciferin is made available for bioluminescence enzymatic reaction by cleavage with activated caspase-3, and therefore it is assumed to be capable of reporting and measuring apoptosis. Apoptosis was investigated for 28 days after stroke in mice which ubiquitously expressed the firefly luciferase transgene. Middle cerebral artery occlusion was performed to achieve ischemic injury, which was followed with magnetic resonance imaging. The scope of apoptosis was determined by bioluminescence with caged Z-DEVD-aminoluciferin, immunofluorescence with activated caspase-3, flow cytometry with annexin-V and TUNEL assay. The linearity of Z-DEVD-aminoluciferin substrate dose effect was shown in the murine brain. Z-DEVD-aminoluciferin was validated as a good tool for monitoring apoptosis following adequate adjustment. By utilizing bioluminescence of Z-DEVD-aminoluciferin after ischemic stroke it was shown that TLR2-deficient mice had lower post-stroke apoptosis than TLR2-expressing wild type mice. In conclusion, Z-DEVD-aminoluciferin could be a valuable tool for apoptosis measurement in living mice. Competing Interests: Declaration of competing interest None. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apoptosis; Bioluminescence; Caged luciferin; Ischemic stroke; Toll-like receptor |
| المشرفين على المادة: | EC 3.4.22.- (Caspase 3) 0 (benzyloxycarbonyl-aspartyl-glutamyl-valyl-aspartyl-aminoluciferin) 0 (Toll-Like Receptor 2) 0 (Oligopeptides) 5TBB02N29K (Firefly Luciferin) |
| تواريخ الأحداث: | Date Created: 20240225 Date Completed: 20240327 Latest Revision: 20240327 |
| رمز التحديث: | 20240327 |
| DOI: | 10.1016/j.jphotobiol.2024.112871 |
| PMID: | 38402658 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2682 |
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| DOI: | 10.1016/j.jphotobiol.2024.112871 |