دورية أكاديمية
[Identification and expression analysis of whole gene family of Isatis indigotica 4-coumarate: CoA ligase].
| العنوان: | [Identification and expression analysis of whole gene family of Isatis indigotica 4-coumarate: CoA ligase]. |
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| المؤلفون: | Zhang KK; State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China., Tan YP; State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China., Xu HH; Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences Beijing 100097, China., Sun SF; State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China., Xiao JC; State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China., Xu ZY; College of Life Sciences, Shandong Normal University Ji'nan 250014, China., Wang CC; College of Life Sciences, Shandong Normal University Ji'nan 250014, China., Zhu HX; Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences Beijing 100097, China., Yang J; State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China., Li DY; Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences Beijing 100097, China., Tang JF; State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China. |
| المصدر: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2024 Jan; Vol. 49 (2), pp. 361-369. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Country of Publication: China NLM ID: 8913656 Publication Model: Print Cited Medium: Print ISSN: 1001-5302 (Print) Linking ISSN: 10015302 NLM ISO Abbreviation: Zhongguo Zhong Yao Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Original Publication: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989- |
| مواضيع طبية MeSH: | Ligases*/genetics , Isatis*/genetics, Promoter Regions, Genetic ; Plants/metabolism ; Flavonoids ; Coenzyme A Ligases/genetics ; Coenzyme A Ligases/chemistry ; Coenzyme A Ligases/metabolism |
| مستخلص: | The 4-coumarate: CoA ligase(4CL) is a key enzyme in the upstream pathway of phenylpropanoids such as flavonoids, soluble phenolic esters, lignans, and lignins in plants. In this study, 13 4CL family members of Arabidopsis thaliana were used as reference sequences to identify the 4CL gene family candidate members of Isatis indigotica from the reported I. indigotica genome. Further bioinformatics analysis and analysis of the expression pattern of 4CL genes and the accumulation pattern of flavonoids were carried out. Thirteen 4CL genes were obtained, named Ii4CL1-Ii4CL13, which were distributed on chromosomes 1, 2, 3, 4, and 6. The analysis of the gene structure and conserved structural domains revealed the intron number of I. indigotica 4CL genes was between 1 and 12 and the protein structural domains were highly conserved. Cis-acting element analysis showed that there were multiple response elements in the promoter sequence of I. indigotica 4CL gene family, and jasmonic acid had the largest number of reaction elements. The collinearity analysis showed that there was a close relationship between the 4CL gene family members of I. indigotica and A. thaliana. As revealed by qPCR results, the expression analysis of the 4CL gene family showed that 10 4CL genes had higher expression levels in the aboveground part of I. indigotica. The content assay of flavonoids in different parts of I. indigotica showed that flavonoids were mainly accumulated in the aboveground part of plants. This study provides a basis for further investigating the roles of the 4CL gene family involved in the biosynthesis of flavonoids in I. indigotica. |
| فهرسة مساهمة: | Keywords: 4-coumarate: CoA ligase; Isatis indigotica; bioinformatics analysis; flavonoids |
| المشرفين على المادة: | EC 6.- (Ligases) 0 (Flavonoids) EC 6.2.1.- (Coenzyme A Ligases) |
| تواريخ الأحداث: | Date Created: 20240225 Date Completed: 20240227 Latest Revision: 20240227 |
| رمز التحديث: | 20240227 |
| DOI: | 10.19540/j.cnki.cjcmm.20230902.103 |
| PMID: | 38403312 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1001-5302 |
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| DOI: | 10.19540/j.cnki.cjcmm.20230902.103 |