Evaluation of a novel Tc-24 recombinant antigen ELISA for serologic testing for Trypanosoma cruzi in dogs.
| العنوان: | Evaluation of a novel Tc-24 recombinant antigen ELISA for serologic testing for Trypanosoma cruzi in dogs. |
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| المؤلفون: | Mejia R; Department of Pediatrics - Tropical Medicine, Baylor College of Medicine, Houston, Texas., Verocai GG; Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas., Mosley IA; Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas., Zhan B; Department of Pediatrics - Tropical Medicine, Baylor College of Medicine, Houston, Texas., Vongthavaravat L; Department of Pediatrics - Tropical Medicine, Baylor College of Medicine, Houston, Texas., Busselman RE; Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas., Hamer SA; Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2024 Feb 14. Date of Electronic Publication: 2024 Feb 14. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | Chagas disease is a parasitic infection caused by Trypanosoma cruzi . Diagnosis of chronic Chagas disease in dogs relies on limited serological test options. This study used a new Tc-24 recombinant antigen ELISA on an archival set of 70 dog serum samples from multi-dog kennel environments in Texas subjected to three existing Chagas serological tests. Tc-24 ELISA produced a quantitative result and could detect anti- T. cruzi antibodies in dogs with high sensitivity and specificity. Comparing individual tests to Tc-24 ELISA resulted in strong associations and correlations, which suggest that Tc-24 ELISA is a reliable and accurate diagnostic tool for dogs with a single test. |
| References: | Vet Parasitol. 2008 Apr 15;152(3-4):249-56. (PMID: 18255233) Exp Parasitol. 1997 Jul;86(3):200-5. (PMID: 9225770) J Vet Diagn Invest. 2023 Nov;35(6):704-709. (PMID: 37670473) Clin Infect Dis. 2020 Dec 17;71(10):e580-e586. (PMID: 32155244) PLoS Negl Trop Dis. 2021 Nov 10;15(11):e0009935. (PMID: 34758049) Sci Data. 2017 Apr 11;4:170050. (PMID: 28398292) Clin Vaccine Immunol. 2007 Aug;14(8):1045-9. (PMID: 17522327) Adv Parasitol. 2017;97:1-45. (PMID: 28325368) Am J Trop Med Hyg. 2009 Sep;81(3):390-5. (PMID: 19706902) PLoS Negl Trop Dis. 2020 Dec 17;14(12):e0008932. (PMID: 33332357) Evol Appl. 2020 Sep 10;13(10):2663-2672. (PMID: 33294015) Vet Parasitol. 2009 Nov 12;165(3-4):241-7. (PMID: 19647943) J Clin Microbiol. 2021 Nov 18;59(12):e0106221. (PMID: 34469183) Exp Parasitol. 2016 Jul;166:44-50. (PMID: 26995535) Fluids Barriers CNS. 2017 Jun 7;14(Suppl 1):15. (PMID: 28929972) |
| معلومات مُعتمدة: | R21 AI171477 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: Chagas disease; Dogs; Tc-24; Texas; Trypanosoma cruzi |
| تواريخ الأحداث: | Date Created: 20240226 Latest Revision: 20240301 |
| رمز التحديث: | 20240301 |
| مُعرف محوري في PubMed: | PMC10888942 |
| DOI: | 10.1101/2024.02.12.579969 |
| PMID: | 38405948 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2024.02.12.579969 |
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