دورية أكاديمية
Dynein and dynactin move long-range but are delivered separately to the axon tip.
| العنوان: | Dynein and dynactin move long-range but are delivered separately to the axon tip. |
|---|---|
| المؤلفون: | Fellows AD; Division of Structural Studies, Medical Research Council Laboratory of Molecular Biology, Cambridge, UK., Bruntraeger M; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Burgold T; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Bassett AR; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Carter AP; Division of Structural Studies, Medical Research Council Laboratory of Molecular Biology, Cambridge, UK. |
| المصدر: | The Journal of cell biology [J Cell Biol] 2024 May 06; Vol. 223 (5). Date of Electronic Publication: 2024 Feb 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-8140 (Electronic) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York : Rockefeller University Press |
| مواضيع طبية MeSH: | Axonal Transport* , Axons* , Dynactin Complex*/genetics , Dyneins*/genetics , Neurons*, Humans ; Neural Stem Cells |
| مستخلص: | Axonal transport is essential for neuronal survival. This is driven by microtubule motors including dynein, which transports cargo from the axon tip back to the cell body. This function requires its cofactor dynactin and regulators LIS1 and NDEL1. Due to difficulties imaging dynein at a single-molecule level, it is unclear how this motor and its regulators coordinate transport along the length of the axon. Here, we use a neuron-inducible human stem cell line (NGN2-OPTi-OX) to endogenously tag dynein components and visualize them at a near-single molecule regime. In the retrograde direction, we find that dynein and dynactin can move the entire length of the axon (>500 µm). Furthermore, LIS1 and NDEL1 also undergo long-distance movement, despite being mainly implicated with the initiation of dynein transport. Intriguingly, in the anterograde direction, dynein/LIS1 moves faster than dynactin/NDEL1, consistent with transport on different cargos. Therefore, neurons ensure efficient transport by holding dynein/dynactin on cargos over long distances but keeping them separate until required. (© 2024 MRC Laboratory of Molecular Biology.) |
| References: | Methods. 2017 Feb 15;115:80-90. (PMID: 27713081) EMBO J. 2016 Feb 1;35(3):302-18. (PMID: 26758546) ACS Chem Biol. 2008 Jun 20;3(6):373-82. (PMID: 18533659) Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):141-4. (PMID: 8552592) J Neurosci. 1996 Nov 1;16(21):6742-52. (PMID: 8824315) Dev Neurobiol. 2015 Jul;75(7):757-77. (PMID: 25404503) Elife. 2023 Feb 02;12:. (PMID: 36729040) J Cell Biol. 2021 Jul 5;220(7):. (PMID: 34014261) Elife. 2021 Jan 20;10:. (PMID: 33470930) Nat Cell Biol. 2020 May;22(5):570-578. (PMID: 32341547) Nat Methods. 2022 Jul;19(7):829-832. (PMID: 35654950) J Biol Chem. 2023 Jun;299(6):104735. (PMID: 37086789) Cell. 2013 Jan 17;152(1-2):172-82. (PMID: 23332753) Elife. 2022 Jan 07;11:. (PMID: 34994688) Mol Biol Cell. 1999 Nov;10(11):3717-28. (PMID: 10564267) Elife. 2017 Apr 13;6:. (PMID: 28406398) EMBO J. 2017 Nov 15;36(22):3387-3404. (PMID: 29038173) J Cell Biol. 2018 Sep 3;217(9):2977-2979. (PMID: 30115668) J Cell Biol. 2019 Nov 4;218(11):3630-3646. (PMID: 31562232) FEBS Lett. 2018 Aug;592(16):2716-2724. (PMID: 30055048) J Cell Biol. 2014 Mar 17;204(6):989-1007. (PMID: 24637326) Neuron. 2011 May 12;70(3):441-54. (PMID: 21555071) Neuron. 2013 Jun 5;78(5):785-98. (PMID: 23764284) J Phys D Appl Phys. 2019 Feb 6;52(6):064002. (PMID: 30799881) Nat Cell Biol. 2020 May;22(5):518-525. (PMID: 32341549) J Cell Biol. 2012 Jun 25;197(7):971-82. (PMID: 22711696) Stem Cell Reports. 2017 Apr 11;8(4):803-812. (PMID: 28344001) J Biol Chem. 2017 Jul 21;292(29):12245-12255. (PMID: 28576829) J Cell Biol. 2019 Sep 2;218(9):2982-3001. (PMID: 31320392) JACS Au. 2021 May 24;1(5):690-696. (PMID: 34056637) Nat Rev Mol Cell Biol. 2014 Sep;15(9):615-28. (PMID: 25118718) Nat Rev Neurol. 2019 Dec;15(12):691-703. (PMID: 31558780) Nat Protoc. 2006;1(4):2128-36. (PMID: 17487204) Neuron. 2014 Oct 22;84(2):292-309. (PMID: 25374356) EMBO Rep. 2020 Mar 4;21(3):e49129. (PMID: 32030864) Stem Cell Reports. 2022 Jan 11;17(1):14-34. (PMID: 34971564) J Cell Biol. 2023 Jul 3;222(7):. (PMID: 37115157) J Neurosci. 2022 Nov 9;42(45):8524-8541. (PMID: 36167783) Methods Mol Biol. 2019;1961:153-183. (PMID: 30912046) Cell. 2016 Feb 11;164(4):722-34. (PMID: 26853472) Biochim Biophys Acta. 2000 Mar 17;1496(1):76-88. (PMID: 10722878) Neuron. 2016 Jun 1;90(5):1000-15. (PMID: 27210554) Nat Rev Mol Cell Biol. 2018 Jun;19(6):382-398. (PMID: 29662141) J Cell Sci. 2010 Jan 15;123(Pt 2):202-12. (PMID: 20048338) Nat Commun. 2023 Mar 13;14(1):1376. (PMID: 36914620) Biochemistry. 1989 Nov 14;28(23):9006-12. (PMID: 2481499) Dev Cell. 2004 Jun;6(6):815-29. (PMID: 15177030) Nat Commun. 2021 Jul 28;12(1):4578. (PMID: 34321481) J Cell Biol. 2024 Mar 4;223(3):. (PMID: 38240798) Nat Cell Biol. 2020 May;22(5):559-569. (PMID: 32341548) Nat Cell Biol. 2010 Dec;12(12):1228-34. (PMID: 21102439) Nat Commun. 2016 Aug 04;7:12259. (PMID: 27489054) Stem Cell Reports. 2017 Oct 10;9(4):1221-1233. (PMID: 28966121) J Cell Biol. 2012 Feb 20;196(4):407-17. (PMID: 22331844) Elife. 2020 Jul 21;9:. (PMID: 32692650) Nat Rev Mol Cell Biol. 2022 Nov;23(11):699-714. (PMID: 35637414) J Cell Biol. 2008 Jun 16;181(6):1027-39. (PMID: 18559670) Cell. 2011 Feb 18;144(4):551-65. (PMID: 21335237) Traffic. 2020 Jan;21(1):138-155. (PMID: 31603614) J Cell Biol. 2009 Dec 28;187(7):1071-82. (PMID: 20038680) EMBO J. 2007 Mar 21;26(6):1475-86. (PMID: 17332754) J Cell Sci. 1999 May;112 ( Pt 10):1437-47. (PMID: 10212138) Nat Commun. 2023 Sep 20;14(1):5833. (PMID: 37730751) EMBO J. 2008 Oct 8;27(19):2471-83. (PMID: 18784752) Cell. 2013 Jun 20;153(7):1526-36. (PMID: 23791180) |
| معلومات مُعتمدة: | 210711/Z/18/Z United Kingdom WT_ Wellcome Trust; MC_UP_A025_1011 United Kingdom MRC_ Medical Research Council |
| المشرفين على المادة: | 0 (Dynactin Complex) EC 3.6.4.2 (Dyneins) EC 3.1.1.47 (PAFAH1B1 protein, human) 0 (NDEL1 protein, human) |
| تواريخ الأحداث: | Date Created: 20240226 Date Completed: 20240227 Latest Revision: 20240229 |
| رمز التحديث: | 20240229 |
| مُعرف محوري في PubMed: | PMC10896695 |
| DOI: | 10.1083/jcb.202309084 |
| PMID: | 38407313 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1540-8140 |
|---|---|
| DOI: | 10.1083/jcb.202309084 |