دورية أكاديمية
Proteomic Profiles of Human Arterioles Isolated From Fresh Adipose Tissue or Following Overnight Storage.
| العنوان: | Proteomic Profiles of Human Arterioles Isolated From Fresh Adipose Tissue or Following Overnight Storage. |
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| المؤلفون: | Pandey R; Center of Systems Molecular Medicine, Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin; Department of Physiology, University of Arizona College of Medicine-Tucson, Tucson, Arizona., Roberts ML; Division of Cardiovascular Medicine, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin., Wang J; Division of Cardiovascular Medicine, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin., Pereckas M; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin., Jensen D; Division of Cardiovascular Medicine, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin., Greene AS; The Jackson Laboratory, Bar Harbor, Maine., Widlansky ME; Division of Cardiovascular Medicine, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin. Electronic address: mwidlans@mcw.edu., Liang M; Center of Systems Molecular Medicine, Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin; Department of Physiology, University of Arizona College of Medicine-Tucson, Tucson, Arizona. Electronic address: mliang1@arizona.edu. |
| المصدر: | Laboratory investigation; a journal of technical methods and pathology [Lab Invest] 2024 May; Vol. 104 (5), pp. 102036. Date of Electronic Publication: 2024 Feb 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc Country of Publication: United States NLM ID: 0376617 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-0307 (Electronic) Linking ISSN: 00236837 NLM ISO Abbreviation: Lab Invest Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [New York] : Elsevier Inc. Original Publication: Baltimore : Williams & Wilkins |
| مواضيع طبية MeSH: | Proteomics*/methods , Adipose Tissue*/metabolism , Adipose Tissue*/blood supply, Humans ; Arterioles/metabolism ; Proteome/metabolism ; Proteome/analysis ; Female ; Male ; Adult ; Middle Aged |
| مستخلص: | Arterioles are key determinants of the total peripheral vascular resistance, which, in turn, is a key determinant of arterial blood pressure. However, the amount of protein available from one isolated human arteriole may be less than 5 μg, making proteomic analysis challenging. In addition, obtaining human arterioles requires manual dissection of unfrozen clinical specimens. This limits its feasibility, especially for powerful multicenter clinical studies in which clinical specimens need to be shipped overnight to a research laboratory for arteriole isolation. We performed a study to address low-input, test overnight tissue storage and develop a reference human arteriolar proteomic profile. In tandem mass tag proteomics, use of a booster channel consisting of human induced pluripotent stem cell-derived endothelial and vascular smooth muscle cells (1:5 ratio) increased the number of proteins detected in a human arteriole segment with a false discovery rate of <0.01 from 1051 to more than 3000. The correlation coefficient of proteomic profile was similar between replicate arterioles isolated freshly, following cold storage, or before and after the cold storage (1-way analysis of variance; P = .60). We built a human arteriolar proteomic profile consisting of 3832 proteins based on the analysis of 12 arteriole samples from 3 subjects. Of 1945 blood pressure-relevant proteins that we curated, 476 (12.5%) were detected in the arteriolar proteome, which was a significant overrepresentation (χ 2 test; P < .05). These findings demonstrate that proteomic analysis is feasible with arterioles isolated from human adipose tissue following cold overnight storage and provide a reference human arteriolar proteome profile highly valuable for studies of arteriole-related traits. (Copyright © 2024 United States & Canadian Academy of Pathology. Published by Elsevier Inc. All rights reserved.) |
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| معلومات مُعتمدة: | R01 HL144098 United States HL NHLBI NIH HHS; P01 HL149620 United States HL NHLBI NIH HHS; R01 HL121233 United States HL NHLBI NIH HHS; K24 HL152143 United States HL NHLBI NIH HHS; R38 HL167238 United States HL NHLBI NIH HHS; R38 HL143561 United States HL NHLBI NIH HHS |
| فهرسة مساهمة: | Keywords: arterioles; blood pressure; human; proteomics; storage |
| تواريخ الأحداث: | Date Created: 20240226 Date Completed: 20240516 Latest Revision: 20240519 |
| رمز التحديث: | 20240519 |
| مُعرف محوري في PubMed: | PMC11098693 |
| DOI: | 10.1016/j.labinv.2024.102036 |
| PMID: | 38408704 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1530-0307 |
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| DOI: | 10.1016/j.labinv.2024.102036 |