دورية أكاديمية
أعرض في EDS

Serum Extracellular Vesicle Protein Profiling for Prediction of Corneal Transplant Rejection.

التفاصيل البيبلوغرافية
العنوان: Serum Extracellular Vesicle Protein Profiling for Prediction of Corneal Transplant Rejection.
المؤلفون: Lee HJ; Department of Ophthalmology, Seoul National University College of Medicine, Jongno-gu, Seoul, Korea.; Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, Seoul National University Hospital, Jongno-gu, Seoul, Korea., Bae EH; Department of Cell Biology and Genetics, Institute for Regenerative Medicine, School of Medicine, Texas A&M University, College Station, TX., Choi JM; Department of Biochemistry, Baylor College of Medicine, Houston, TX., Kim H; Department of Cell Biology and Genetics, Institute for Regenerative Medicine, School of Medicine, Texas A&M University, College Station, TX., Kim HJ; Department of Ophthalmology, Seoul National University College of Medicine, Jongno-gu, Seoul, Korea.; Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, Seoul National University Hospital, Jongno-gu, Seoul, Korea., Barreda H; Department of Cell Biology and Genetics, Institute for Regenerative Medicine, School of Medicine, Texas A&M University, College Station, TX., Jung SY; Department of Biochemistry, Baylor College of Medicine, Houston, TX., Oh JY; Department of Ophthalmology, Seoul National University College of Medicine, Jongno-gu, Seoul, Korea.; Laboratory of Ocular Regenerative Medicine and Immunology, Biomedical Research Institute, Seoul National University Hospital, Jongno-gu, Seoul, Korea., Lee RH; Department of Cell Biology and Genetics, Institute for Regenerative Medicine, School of Medicine, Texas A&M University, College Station, TX.
المصدر: Transplantation [Transplantation] 2024 Jun 01; Vol. 108 (6), pp. 1368-1375. Date of Electronic Publication: 2024 Feb 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 0132144 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1534-6080 (Electronic) Linking ISSN: 00411337 NLM ISO Abbreviation: Transplantation Subsets: MEDLINE
أسماء مطبوعة: Publication: Hagerstown, MD : Lippincott Williams & Wilkins
Original Publication: Baltimore, Williams & Wilkins.
مواضيع طبية MeSH: Graft Rejection*/blood , Graft Rejection*/immunology , Graft Rejection*/diagnosis , Extracellular Vesicles*/metabolism , Mice, Inbred C57BL* , Biomarkers*/blood , Corneal Transplantation* , Proteomics*/methods , Mice, Inbred BALB C*, Animals ; Mice ; Graft Survival ; Disease Models, Animal ; Predictive Value of Tests ; Male
مستخلص: Background: Corneal transplantation is the most common transplant procedure worldwide. Despite immune and angiogenic privilege of the cornea, 50% to 70% of corneal transplants fail in high-risk recipients, primarily because of immune rejection. Therefore, it is crucial to identify predictive biomarkers of rejection to improve transplant survival.
Methods: In search for predictive biomarkers, we performed proteomics analysis of serum extracellular vesicles (EVs) in a fully major histocompatibility complex-mismatched (C57BL/6-to-BALB/c) murine corneal transplantation model, wherein 50% of transplants undergo rejection by day 28 following transplantation.
Results: Our time course study revealed a decrease in the number of serum EVs on day 1, followed by a gradual increase by day 7. A comparative analysis of proteomics profiles of EVs from transplant recipients with rejection (rejectors) and without rejection (nonrejectors) found a distinct enrichment of histocompatibility 2, Q region locus 2, which is a part of major histocompatibility complex-class I of donor C57BL/6 mice, in day 7 EVs of rejectors, compared with nonrejectors, syngeneic controls, or naïve mice. In contrast, serum amyloid A2, a protein induced in response to injury, was increased in day 7 EVs of nonrejectors.
Conclusions: Our findings offer noninvasive EV-based potential biomarkers for predicting corneal allograft rejection or tolerance.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)
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معلومات مُعتمدة: R01 EY029350 United States EY NEI NIH HHS; R21 DE027457 United States DE NIDCR NIH HHS
المشرفين على المادة: 0 (Biomarkers)
تواريخ الأحداث: Date Created: 20240227 Date Completed: 20240529 Latest Revision: 20240605
رمز التحديث: 20240605
مُعرف محوري في PubMed: PMC11136603
DOI: 10.1097/TP.0000000000004946
PMID: 38409732
قاعدة البيانات: MEDLINE
الوصف
تدمد:1534-6080
DOI:10.1097/TP.0000000000004946