دورية أكاديمية
Cytokine expression in subjects with Mycobacterium avium ssp. paratuberculosis positive blood cultures and a meta-analysis of cytokine expression in Crohn's disease.
| العنوان: | Cytokine expression in subjects with Mycobacterium avium ssp. paratuberculosis positive blood cultures and a meta-analysis of cytokine expression in Crohn's disease. |
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| المؤلفون: | Kuenstner JT; Department of Biology, Abilene Christian University, Abilene, TX, United States., Xu Q; Department of Biology, Abilene Christian University, Abilene, TX, United States., Bull TJ; Institute of Infection and Immunity, St. George's University of London, London, United Kingdom., Foddai ACG; School of Health and Life Sciences, Teesside University, Middlesbrough, United Kingdom., Grant IR; School of Biological Sciences, Queen's University Belfast, Belfast, United Kingdom., Naser SA; Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, United States., Potula R; Lewis Katz School of Medicine, Temple University, Philadelphia, PA, United States., Zhang P; PZM Diagnostics, Charleston, WV, United States., Shafran I; Retired, Winter Park, FL, United States., Akhanli SE; Department of Statistics, Muğla Sıtkı Koçman University, Muğla, Türkiye., Khaiboullina S; Department of Microbiology and Immunology, University of Nevada, Reno, NV, United States.; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia., Kruzelock R; Department of Biology, Abilene Christian University, Abilene, TX, United States. |
| المصدر: | Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 Feb 13; Vol. 14, pp. 1327969. Date of Electronic Publication: 2024 Feb 13 (Print Publication: 2024). |
| نوع المنشور: | Meta-Analysis; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Media SA |
| مواضيع طبية MeSH: | Crohn Disease*/microbiology , Paratuberculosis*/microbiology , Mycobacterium avium subsp. paratuberculosis* , Tuberculosis*, Animals ; Humans ; Interleukin-17 ; Cytokines ; Tumor Necrosis Factor-alpha ; Blood Culture |
| مستخلص: | Objectives: 1) Culture Mycobacterium avium ssp. paratuberculosis (MAP)from blood, 2) assess infection persistence, 3) determine Crohn's disease (CD) cytokine expression, 4) compare CD cytokine expression to tuberculosis, and 5) perform a meta-analysis of cytokine expression in CD. Methods: The Temple University/Abilene Christian University (TU/ACU) study had a prospective case control design with 201 subjects including 61 CD patients and 140 non-CD controls. The culture methods included MGIT, TiKa and Pozzato broths, and were deemed MAP positive, if IS900 PCR positive. A phage amplification assay was also performed to detect MAP. Cytokine analysis of the TU/ACU samples was performed using Simple Plex cytokine reagents on the Ella ELISA system. Statistical analyses were done after log transformation using the R software package. The meta-analysis combined three studies. Results: Most subjects had MAP positive blood cultures by one or more methods in 3 laboratories. In our cytokine study comparing CD to non-CD controls, IL-17, IFNγ and TNFα were significantly increased in CD, but IL-2, IL-5, IL-10 and GM-CSF were not increased. In the meta-analysis, IL-6, IL-8 and IL-12 were significantly increased in the CD patients. Conclusion: Most subjects in our sample had MAP infection and 8 of 9 subjects remained MAP positive one year later indicating persistent infection. While not identical, cytokine expression patterns in MAP culture positive CD patients in the TU/ACU study showed similarities (increased IL-17, IFNγ and TNFα) to patterns of patients with Tuberculosis in other studies, indicating the possibilities of similar mechanisms of pathogen infection and potential strategies for treatment. Competing Interests: TB is a director of TiKa Diagnostics Ltd., proprietor of the TiKa mycobacterial growth media used in this study. IG, AF, JK, RP, TB, SN, and IS have a proprietary interest in a pending patent for the MAP phage assay. JK, RP, and PZ have a proprietary interest in pending patents for MAP antibody assays. PZ is also the principal owner of PZM Diagnostics. IG is a director of Rapid-Myco Technologies Limited, a company seeking to commercialize the phagomagnetic separation-qPCR assay for viable MAP/other mycobacteria. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Kuenstner, Xu, Bull, Foddai, Grant, Naser, Potula, Zhang, Shafran, Akhanli, Khaiboullina and Kruzelock.) |
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| فهرسة مساهمة: | Keywords: Crohn’s disease; MAP; autoimmune disease; bacteremia; inflammatory bowel disease (IBD) |
| المشرفين على المادة: | 0 (Interleukin-17) 0 (Cytokines) 0 (Tumor Necrosis Factor-alpha) |
| تواريخ الأحداث: | Date Created: 20240228 Date Completed: 20240229 Latest Revision: 20240229 |
| رمز التحديث: | 20240229 |
| مُعرف محوري في PubMed: | PMC10896875 |
| DOI: | 10.3389/fcimb.2024.1327969 |
| PMID: | 38415011 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2235-2988 |
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| DOI: | 10.3389/fcimb.2024.1327969 |