دورية أكاديمية
Acoustic Wave-Induced Stroboscopic Optical Mechanotyping of Adherent Cells.
| العنوان: | Acoustic Wave-Induced Stroboscopic Optical Mechanotyping of Adherent Cells. |
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| المؤلفون: | Combriat T; Njord Centre, Department of Physics, University of Oslo, P.O. Box 1048 Blindern, Oslo, 0316, Norway.; Hybrid Technology Hub, University of Oslo, Institute of Basic Medical Sciences P.O. Box 1110 Blindern, Oslo, 0317, Norway.; Center for Computing in Science Education, University of Oslo, P.O. Box 1048 Blindern, Oslo, 0316, Norway., Olsen PA; Hybrid Technology Hub, University of Oslo, Institute of Basic Medical Sciences P.O. Box 1110 Blindern, Oslo, 0317, Norway.; Department of Immunology and Transfusion Medicine, Oslo University Hospital, P.O. Box 4950, Nydalen, Oslo, 0424, Norway., Låstad SB; Njord Centre, Department of Physics, University of Oslo, P.O. Box 1048 Blindern, Oslo, 0316, Norway., Malthe-Sørenssen A; Njord Centre, Department of Physics, University of Oslo, P.O. Box 1048 Blindern, Oslo, 0316, Norway.; Center for Computing in Science Education, University of Oslo, P.O. Box 1048 Blindern, Oslo, 0316, Norway., Krauss S; Hybrid Technology Hub, University of Oslo, Institute of Basic Medical Sciences P.O. Box 1110 Blindern, Oslo, 0317, Norway.; Department of Immunology and Transfusion Medicine, Oslo University Hospital, P.O. Box 4950, Nydalen, Oslo, 0424, Norway., Dysthe DK; Njord Centre, Department of Physics, University of Oslo, P.O. Box 1048 Blindern, Oslo, 0316, Norway. |
| المصدر: | Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Apr; Vol. 11 (16), pp. e2307929. Date of Electronic Publication: 2024 Feb 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: WILEY-VCH Country of Publication: Germany NLM ID: 101664569 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2198-3844 (Electronic) Linking ISSN: 21983844 NLM ISO Abbreviation: Adv Sci (Weinh) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Weinheim : WILEY-VCH, [2014]- |
| مواضيع طبية MeSH: | Stroboscopy*/methods, Humans ; Cell Adhesion/physiology ; Sound ; Optical Imaging/methods ; Animals |
| مستخلص: | In this study, a novel, high content technique using a cylindrical acoustic transducer, stroboscopic fast imaging, and homodyne detection to recover the mechanical properties (dynamic shear modulus) of living adherent cells at low ultrasonic frequencies is presented. By analyzing the micro-oscillations of cells, whole populations are simultaneously mechanotyped with sub-cellular resolution. The technique can be combined with standard fluorescence imaging allowing to further cross-correlate biological and mechanical information. The potential of the technique is demonstrated by mechanotyping co-cultures of different cell types with significantly different mechanical properties. (© 2024 The Authors. Advanced Science published by Wiley‐VCH GmbH.) |
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| معلومات مُعتمدة: | Livsvitenskap, Universitetet i Oslo: ABINO; 262613 Norges Forskningsråd |
| فهرسة مساهمة: | Keywords: biophysics; cell mechanics; live cell imaging; ultrasound; viscoelasticity |
| تواريخ الأحداث: | Date Created: 20240228 Date Completed: 20240424 Latest Revision: 20240426 |
| رمز التحديث: | 20240426 |
| مُعرف محوري في PubMed: | PMC11040383 |
| DOI: | 10.1002/advs.202307929 |
| PMID: | 38417124 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2198-3844 |
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| DOI: | 10.1002/advs.202307929 |