دورية أكاديمية
Research Note: Clostridium perfringens NetB and CnaA neutralizing nanobodies in feed reduce the incidence of poultry necrotic enteritis.
| العنوان: | Research Note: Clostridium perfringens NetB and CnaA neutralizing nanobodies in feed reduce the incidence of poultry necrotic enteritis. |
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| المؤلفون: | Loutet SA; NovoBind Livestock Therapeutics, Vancouver, British Columbia V6E 0C3, Canada., Cheung S; NovoBind Livestock Therapeutics, Vancouver, British Columbia V6E 0C3, Canada., Zaytsoff S; NovoBind Livestock Therapeutics, Vancouver, British Columbia V6E 0C3, Canada., Hofacre C; Southern Poultry Research Group, Inc., Watkinsville, GA 30677, USA., Jones MK; Southern Poultry Research Group, Inc., Watkinsville, GA 30677, USA., Van Petegem F; Department of Biochemistry and Molecular Biology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada., Abnousi H; NovoBind Livestock Therapeutics, Vancouver, British Columbia V6E 0C3, Canada. Electronic address: info@novobind.com. |
| المصدر: | Poultry science [Poult Sci] 2024 Apr; Vol. 103 (4), pp. 103578. Date of Electronic Publication: 2024 Feb 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 0401150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3171 (Electronic) Linking ISSN: 00325791 NLM ISO Abbreviation: Poult Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : [Cambridge, UK] : Elsevier Original Publication: Champaign Il : Poultry Science Association |
| مواضيع طبية MeSH: | Single-Domain Antibodies* , Clostridium Infections*/prevention & control , Clostridium Infections*/veterinary , Enteritis*/prevention & control , Enteritis*/veterinary , Poultry Diseases*/prevention & control , Poultry Diseases*/microbiology, Animals ; Clostridium perfringens/genetics ; Poultry ; Incidence ; Chickens |
| مستخلص: | Necrotic enteritis is a devastating disease to poultry caused by the bacterium Clostridium perfringens. As a novel approach to combating poultry necrotic enteritis, we identified and characterized several hundred single domain antibody fragments (or nanobodies) capable of binding either the NetB toxin or the collagen-binding adhesin (CnaA) of C. perfringens. Many of the nanobodies could neutralize the in vitro functions of NetB or CnaA with inhibitory concentrations in the nanomolar range. The nanobodies were also screened for proteolytic stability in an extract derived from gastrointestinal tract fluids of chickens. A collection of 6 nanobodies (4 targeting NetB and 2 targeting CnaA) with high neutralizing activity and high gastrointestinal tract extract stability were expressed and secreted by Pichia pastoris or Bacillus subtilis. Chickens were given a feed with 1 of the 2 nanobody-containing groups: 1) nanobody-containing P. pastoris supernatants that were semi-purified, lyophilized, and enterically coated, or 2) B. subtilis spores from strains containing the nanobody genes. Compared to untreated chickens (23.75% mortality), mortality of chickens receiving feed modified with the P. pastoris and B. subtilis products decreased to 11.25 and 7.5%, respectively. These results offer a new opportunity to improve the control of poultry necrotic enteritis by incorporating highly specific nanobodies or bacteria expressing these nanobodies directly into chicken feed. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Clostridium perfringens; feed; nanobody; necrotic enteritis |
| المشرفين على المادة: | 0 (Single-Domain Antibodies) |
| تواريخ الأحداث: | Date Created: 20240228 Date Completed: 20240322 Latest Revision: 20240322 |
| رمز التحديث: | 20240322 |
| مُعرف محوري في PubMed: | PMC10909890 |
| DOI: | 10.1016/j.psj.2024.103578 |
| PMID: | 38417327 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1525-3171 |
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| DOI: | 10.1016/j.psj.2024.103578 |