دورية أكاديمية
أعرض في EDS

Ehf and Fezf2 regulate late medullary thymic epithelial cell and thymic tuft cell development.

التفاصيل البيبلوغرافية
العنوان: Ehf and Fezf2 regulate late medullary thymic epithelial cell and thymic tuft cell development.
المؤلفون: Lammers S; Institute for Theoretical Physics, Heidelberg University, Heidelberg, Germany., Barrera V; Bioinformatics Core, Harvard T.H. Chan School of Public Health, Boston, MA, United States., Brennecke P; Department of Genetics, Stanford University, School of Medicine, Stanford, CA, United States.; Stanford Genome Technology Center, Stanford University, Stanford, CA, United States., Miller C; Diabetes Center, University of California, San Francisco (UCSF), San Francisco, CA, United States., Yoon J; Bioinformatics Core, Harvard T.H. Chan School of Public Health, Boston, MA, United States., Balolong J; Diabetes Center, University of California, San Francisco (UCSF), San Francisco, CA, United States., Anderson MS; Diabetes Center, University of California, San Francisco (UCSF), San Francisco, CA, United States., Ho Sui S; Bioinformatics Core, Harvard T.H. Chan School of Public Health, Boston, MA, United States., Steinmetz LM; Department of Genetics, Stanford University, School of Medicine, Stanford, CA, United States.; Stanford Genome Technology Center, Stanford University, Stanford, CA, United States.; Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany., von Andrian UH; Department of Immunology & HMS Center for Immune Imaging, Harvard Medical School, Boston, MA, United States.; The Ragon Institute of MGH, MIT and Harvard, Cambridge, MA, United States., Rattay K; Department of Immunology & HMS Center for Immune Imaging, Harvard Medical School, Boston, MA, United States.; Pharmacological Institute, Biochemical Pharmacological Center, University of Marburg, Marburg, Germany.
المصدر: Frontiers in immunology [Front Immunol] 2024 Feb 14; Vol. 14, pp. 1277365. Date of Electronic Publication: 2024 Feb 14 (Print Publication: 2023).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Lausanne : Frontiers Research Foundation]
مواضيع طبية MeSH: Tuft Cells* , Transcription Factors*/metabolism, Gene Expression Regulation ; Epithelial Cells/metabolism ; Peptides/metabolism
مستخلص: Thymic epithelial cells are indispensable for T cell maturation and selection and the induction of central immune tolerance. The self-peptide repertoire expressed by medullary thymic epithelial cells is in part regulated by the transcriptional regulator Aire (Autoimmune regulator) and the transcription factor Fezf2. Due to the high complexity of mTEC maturation stages (i.e., post-Aire, Krt10+ mTECs, and Dclk1+ Tuft mTECs) and the heterogeneity in their gene expression profiles (i.e., mosaic expression patterns), it has been challenging to identify the additional factors complementing the transcriptional regulation. We aimed to identify the transcriptional regulators involved in the regulation of mTEC development and self-peptide expression in an unbiased and genome-wide manner. We used ATAC footprinting analysis as an indirect approach to identify transcription factors involved in the gene expression regulation in mTECs, which we validated by ChIP sequencing. This study identifies Fezf2 as a regulator of the recently described thymic Tuft cells (i.e., Tuft mTECs). Furthermore, we identify that transcriptional regulators of the ELF, ESE, ERF, and PEA3 subfamily of the ETS transcription factor family and members of the Krüppel-like family of transcription factors play a role in the transcriptional regulation of genes involved in late mTEC development and promiscuous gene expression.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Lammers, Barrera, Brennecke, Miller, Yoon, Balolong, Anderson, Ho Sui, Steinmetz, von Andrian and Rattay.)
References: Nature. 2012 May 30;486(7401):74-9. (PMID: 22678282)
Nucleic Acids Res. 2016 Jul 8;44(W1):W160-5. (PMID: 27079975)
Molecules. 2018 Aug 30;23(9):. (PMID: 30200227)
Mech Dev. 2000 Oct;97(1-2):191-5. (PMID: 11025224)
Science. 2002 Nov 15;298(5597):1395-401. (PMID: 12376594)
Front Genet. 2014 Apr 10;5:75. (PMID: 24782889)
Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):E3497-505. (PMID: 23980163)
J Immunol. 2015 Feb 1;194(3):921-8. (PMID: 25552543)
Nat Genet. 2017 Oct;49(10):1522-1528. (PMID: 28805829)
Nucleic Acids Res. 2016 Apr 20;44(7):e67. (PMID: 26704968)
Bioinformatics. 2014 Apr 1;30(7):923-30. (PMID: 24227677)
Nature. 2016 Jan 14;529(7585):226-30. (PMID: 26762460)
Annu Rev Immunol. 2006;24:571-606. (PMID: 16551260)
Int Immunopharmacol. 2015 Nov;29(1):143-7. (PMID: 26102274)
Nat Commun. 2016 Jun 27;7:11938. (PMID: 27346425)
Nat Commun. 2021 Oct 28;12(1):6230. (PMID: 34711828)
Nature. 2018 Jul;559(7715):622-626. (PMID: 30022162)
Cell. 2022 Jul 7;185(14):2542-2558.e18. (PMID: 35714609)
Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15854-9. (PMID: 18836079)
Front Immunol. 2021 Sep 30;12:740047. (PMID: 34659232)
Genome Biol. 2014;15(12):550. (PMID: 25516281)
Eur J Immunol. 2013 Jan;43(1):75-84. (PMID: 23041971)
Nat Rev Immunol. 2014 Jun;14(6):377-91. (PMID: 24830344)
Immunity. 2018 Jun 19;48(6):1258-1270.e6. (PMID: 29884461)
Bioinformatics. 2012 Oct 15;28(20):2678-9. (PMID: 22914218)
PeerJ. 2017 Aug 30;5:e3720. (PMID: 28875074)
PLoS One. 2015 Nov 24;10(11):e0143627. (PMID: 26599758)
Nature. 2015 Jul 23;523(7561):486-90. (PMID: 26083756)
Nat Methods. 2013 Nov;10(11):1093-5. (PMID: 24056876)
EMBO J. 2020 Jan 2;39(1):e101828. (PMID: 31657037)
Science. 2016 Mar 18;351(6279):1329-33. (PMID: 26847546)
J Autoimmun. 2016 Feb;67:65-75. (PMID: 26481130)
Int Immunol. 2022 Jan 1;34(1):45-52. (PMID: 34687536)
Cell Rep. 2016 Apr 19;15(3):651-665. (PMID: 27068467)
Nucleic Acids Res. 2012 Aug;40(15):e115. (PMID: 22730293)
Eur J Immunol. 2015 Aug;45(8):2218-31. (PMID: 25973789)
Sci Rep. 2018 Jan 12;8(1):685. (PMID: 29330484)
J Biol Chem. 2011 May 27;286(21):18641-9. (PMID: 21471212)
Nat Immunol. 2015 Sep;16(9):942-9. (PMID: 26237550)
Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19426-31. (PMID: 20966351)
Cancer Res. 2007 Apr 15;67(8):3919-26. (PMID: 17440107)
F1000Res. 2015 Dec 30;4:1521. (PMID: 26925227)
Science. 2015 May 22;348(6237):910-4. (PMID: 25953818)
Nat Methods. 2015 Oct;12(10):963-965. (PMID: 26280331)
J Biol Chem. 2013 Nov 29;288(48):34304-24. (PMID: 24142692)
Nat Immunol. 2015 Sep;16(9):933-41. (PMID: 26237553)
Nature. 2016 Jan 14;529(7585):221-5. (PMID: 26675736)
Nat Methods. 2013 Dec;10(12):1213-8. (PMID: 24097267)
J Histochem Cytochem. 2003 Feb;51(2):187-98. (PMID: 12533527)
Genome Biol. 2008;9(9):R137. (PMID: 18798982)
Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286)
Annu Rev Immunol. 2017 Apr 26;35:85-118. (PMID: 28226225)
Bioinformatics. 2015 Jul 15;31(14):2382-3. (PMID: 25765347)
Front Immunol. 2012 Mar;3(March):19. (PMID: 22448160)
Mech Dev. 2000 May;93(1-2):201-4. (PMID: 10781957)
Sci Rep. 2016 Jun 01;6:27030. (PMID: 27249108)
J Biol Chem. 2017 Jun 30;292(26):10938-10949. (PMID: 28461336)
J Histochem Cytochem. 1988 Dec;36(12):1511-7. (PMID: 2461413)
Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886)
Nat Biotechnol. 2011 Jan;29(1):24-6. (PMID: 21221095)
J Exp Med. 2010 May 10;207(5):963-71. (PMID: 20404099)
Proc Natl Acad Sci U S A. 2008 Jan 15;105(2):657-62. (PMID: 18180458)
Annu Rev Immunol. 2003;21:139-76. (PMID: 12414722)
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W253-8. (PMID: 17478497)
Immunol Cell Biol. 2016 Mar;94(3):221-3. (PMID: 26975341)
Nat Immunol. 2001 Nov;2(11):1032-9. (PMID: 11600886)
Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):3041-6. (PMID: 21285371)
J Immunol. 2004 Jan 1;172(1):617-24. (PMID: 14688374)
J Histochem Cytochem. 1991 May;39(5):645-53. (PMID: 2016514)
Genome Res. 2014 Dec;24(12):1918-31. (PMID: 25224068)
Development. 2021 Jun 15;148(12):. (PMID: 34180969)
Genome Biol. 2020 Feb 3;21(1):22. (PMID: 32014034)
Nat Rev Immunol. 2020 Apr;20(4):239-253. (PMID: 31804611)
Nat Methods. 2017 Apr;14(4):417-419. (PMID: 28263959)
Trends Immunol. 2017 Nov;38(11):805-816. (PMID: 28830733)
Front Immunol. 2015 Mar 03;6:93. (PMID: 25784915)
Immunity. 2015 Jun 16;42(6):1048-61. (PMID: 26070482)
Nature. 2018 Jul;559(7715):627-631. (PMID: 30022164)
Cell Rep. 2013 Oct 17;5(1):166-79. (PMID: 24095736)
Cell Tissue Res. 2014 Dec;358(3):737-48. (PMID: 25300645)
Cell. 2015 Nov 5;163(4):975-87. (PMID: 26544942)
Nucleic Acids Res. 2009 Jul;37(Web Server issue):W202-8. (PMID: 19458158)
Dev Growth Differ. 2009 Apr;51(3):221-31. (PMID: 19222525)
Nat Immunol. 2020 Aug;21(8):892-901. (PMID: 32601470)
J Exp Med. 2008 Nov 24;205(12):2827-38. (PMID: 19015306)
معلومات مُعتمدة: R01 AI155865 United States AI NIAID NIH HHS; R37 AI097457 United States AI NIAID NIH HHS; P01 HG000205 United States HG NHGRI NIH HHS; R01 GM068717 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: Ehf; Fezf2; Tuft cells; central tolerance; medullary thymic epithelial cell; thymus
المشرفين على المادة: 0 (Transcription Factors)
0 (Peptides)
تواريخ الأحداث: Date Created: 20240229 Date Completed: 20240301 Latest Revision: 20240725
رمز التحديث: 20240726
مُعرف محوري في PubMed: PMC10901246
DOI: 10.3389/fimmu.2023.1277365
PMID: 38420512
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-3224
DOI:10.3389/fimmu.2023.1277365