دورية أكاديمية
Collagen-decorated electrospun scaffolds of unsaturated copolyesters for bone tissue regeneration.
| العنوان: | Collagen-decorated electrospun scaffolds of unsaturated copolyesters for bone tissue regeneration. |
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| المؤلفون: | Madalosso HB; Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Campus Trindade, 88040-900, Florianópolis, Brazil. claudia.sayer@ufsc.br., Guindani C; Chemical Engineering Program/COPPE, Federal University of Rio de Janeiro, Cidade Universitária, CP: 68502, Rio de Janeiro, 21941-972 RJ, Brazil., Maniglia BC; São Carlos Institute of Chemistry, University of São Paulo - USP, Campus São Carlos, 13566-590, São Carlos, SP, Brazil., Hermes de Araújo PH; Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Campus Trindade, 88040-900, Florianópolis, Brazil. claudia.sayer@ufsc.br., Sayer C; Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Campus Trindade, 88040-900, Florianópolis, Brazil. claudia.sayer@ufsc.br. |
| المصدر: | Journal of materials chemistry. B [J Mater Chem B] 2024 Mar 20; Vol. 12 (12), pp. 3047-3062. Date of Electronic Publication: 2024 Mar 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101598493 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-7518 (Electronic) Linking ISSN: 2050750X NLM ISO Abbreviation: J Mater Chem B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge : Royal Society of Chemistry |
| مواضيع طبية MeSH: | Tissue Scaffolds*/chemistry , Cysteine*, Collagen/chemistry ; Bone and Bones ; Bone Regeneration ; Polymers |
| مستخلص: | Many efforts have been devoted to bone tissue to regenerate damaged tissues, and the development of new biocompatible materials that match the biological, mechanical, and chemical features required for this application is crucial. Herein, a collagen-decorated scaffold was prepared via electrospinning using a synthesized unsaturated copolyester (poly(globalide- co -pentadecalactone)), followed by two coupling reactions: thiol-ene functionalization with cysteine and further conjugation via EDC/NHS chemistry with collagen, aiming to design a bone tissue regeneration device with improved hydrophilicity and cell viability. Comonomer ratios were varied, affecting the copolymer's thermal and chemical properties and highlighting the tunable features of this copolyester. Functionalization with cysteine created new carboxyl and amine groups needed for bioconjugation with collagen, which is responsible for providing biological and structural integrity to the extra-cellular matrix. Bioconjugation with collagen turned the scaffold highly hydrophilic, decreasing its contact angle from 107 ± 2° to 0°, decreasing the copolymer crystallinity by 71%, and improving cell viability by 85% compared with the raw scaffold, thus promoting cell growth and proliferation. The highly efficient and biosafe strategy to conjugate polymers and proteins created a promising device for bone repair in tissue engineering. |
| المشرفين على المادة: | K848JZ4886 (Cysteine) 9007-34-5 (Collagen) 0 (Polymers) |
| تواريخ الأحداث: | Date Created: 20240229 Date Completed: 20240321 Latest Revision: 20240321 |
| رمز التحديث: | 20240321 |
| DOI: | 10.1039/d3tb02665e |
| PMID: | 38421173 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2050-7518 |
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| DOI: | 10.1039/d3tb02665e |