دورية أكاديمية
Cellulase-assisted platelet-rich plasma release from nanofibrillated cellulose hydrogel enhances wound healing.
| العنوان: | Cellulase-assisted platelet-rich plasma release from nanofibrillated cellulose hydrogel enhances wound healing. |
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| المؤلفون: | Koivunotko E; Division of Pharmaceutical Biosciences, Drug Research Program, Faculty of Pharmacy, University of Helsinki, 00790 Helsinki, Finland., Koivuniemi R; Division of Pharmaceutical Biosciences, Drug Research Program, Faculty of Pharmacy, University of Helsinki, 00790 Helsinki, Finland., Monola J; Division of Pharmaceutical Biosciences, Drug Research Program, Faculty of Pharmacy, University of Helsinki, 00790 Helsinki, Finland., Harjumäki R; Division of Pharmaceutical Biosciences, Drug Research Program, Faculty of Pharmacy, University of Helsinki, 00790 Helsinki, Finland., Pridgeon CS; Division of Pharmaceutical Biosciences, Drug Research Program, Faculty of Pharmacy, University of Helsinki, 00790 Helsinki, Finland., Madetoja M; Made Consulting Ltd, Tykistökatu 4b, 20520 Turku, Finland., Linden J; Faculty of Veterinary Medicine, Department of Veterinary Biosciences and Finnish Centre for Laboratory Animal Pathology, HiLIFE, University of Helsinki, Helsinki, Finland., Paasonen L; UPM Biomedicals, UPM-Kymmene Corporation, 00100 Helsinki, Finland., Laitinen S; Research and Cell Therapy Services, Finnish Red Cross Blood Service, Kivihaantie 7, 00310 Helsinki, Finland., Yliperttula M; Division of Pharmaceutical Biosciences, Drug Research Program, Faculty of Pharmacy, University of Helsinki, 00790 Helsinki, Finland. Electronic address: marjo.yliperttula@helsinki.fi. |
| المصدر: | Journal of controlled release : official journal of the Controlled Release Society [J Control Release] 2024 Apr; Vol. 368, pp. 397-412. Date of Electronic Publication: 2024 Mar 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8607908 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4995 (Electronic) Linking ISSN: 01683659 NLM ISO Abbreviation: J Control Release Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier Science Publishers, 1984- |
| مواضيع طبية MeSH: | Platelet-Rich Plasma* , Cellulases*/pharmacology, Mice ; Animals ; Hydrogels/pharmacology ; Cellulose ; Wound Healing |
| مستخلص: | Platelet-rich plasma (PRP) is a source of growth factors, which are implicated in active tissue regeneration. However, after transplantation the efficacy of these bioactive compounds is often diminished due to rapid degradation and untargeted localization. For this reason, we evaluated the potential of nanofibrillated cellulose (NFC) hydrogel as a PRP carrier. NFC hydrogel is an animal-free biomaterial that, when doped with cellulase, can assist the release of PRP in a wound site. In this study, we examined the effects of 0.5% (m/v) NFC hydrogel formulations, including PRP and cellulase, on the migration and proliferation of skin cells via an in vitro scratch wound model. The suitability of the 0.8% NFC hydrogel formulations for accelerated wound healing and PRP carrying was studied in vitro in diffusion studies and in vivo in a full-thickness excisional wound model in SKH1 mice. None of the NFC hydrogel formulations with or without PRP and cellulase disturbed the normal cell behavior in vitro, and cellulase was successfully used to degrade NFC. NFC hydrogel slowed fibroblast migration rate in vitro. In vivo, NFC hydrogel treatment showed significantly enhanced re-epithelialization compared to control and supported collagen deposition. In addition, angiogenesis was significantly induced via PRP release after degrading NFC hydrogel with cellulase without abnormal host reaction. This study demonstrates the potential of NFC hydrogel with cellulase as a carrier for PRP with controlled release in future skin tissue engineering applications. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Assisted release; Carrier; Cellulase; Nanofibrillated cellulose hydrogel; Platelet-rich plasma; Wound healing |
| المشرفين على المادة: | 0 (Hydrogels) 9004-34-6 (Cellulose) EC 3.2.1.- (Cellulases) |
| تواريخ الأحداث: | Date Created: 20240229 Date Completed: 20240410 Latest Revision: 20240410 |
| رمز التحديث: | 20240410 |
| DOI: | 10.1016/j.jconrel.2024.02.041 |
| PMID: | 38423475 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4995 |
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| DOI: | 10.1016/j.jconrel.2024.02.041 |