دورية أكاديمية
أعرض في EDS

Effect of cryopreservation on CD4+ T cell subsets in foreskin tissue.

التفاصيل البيبلوغرافية
العنوان: Effect of cryopreservation on CD4+ T cell subsets in foreskin tissue.
المؤلفون: Almomani O; Department of Microbiology and Immunology, Western University, London, Canada., Nnamutete J; Rakai Health Sciences Program, Kalisizo, Uganda., Shao Z; Department of Microbiology and Immunology, Western University, London, Canada., Biribawa VM; UVRI-IAVI HIV Vaccine Program Limited, Entebbe, Uganda., Ssemunywa H; UVRI-IAVI HIV Vaccine Program Limited, Entebbe, Uganda., Namuniina A; UVRI-IAVI HIV Vaccine Program Limited, Entebbe, Uganda., Okech B; UVRI-IAVI HIV Vaccine Program Limited, Entebbe, Uganda., Ulanova S; Department of Microbiology and Immunology, Western University, London, Canada., Zuanazzi D; Department of Microbiology and Immunology, Western University, London, Canada., Liu CM; Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, United States of America., Tobian AAR; Department of Pathology, Johns Hopkins University School of Medicine, Johns Hopkins University, Baltimore, Maryland, United States of America., Galiwango RM; Rakai Health Sciences Program, Kalisizo, Uganda., Kaul R; Departments of Medicine and Immunology, University of Toronto, Toronto, Canada.; University Health Network, Toronto, Canada., Prodger JL; Department of Microbiology and Immunology, Western University, London, Canada.
المصدر: PloS one [PLoS One] 2024 Mar 01; Vol. 19 (3), pp. e0297884. Date of Electronic Publication: 2024 Mar 01 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Foreskin* , HIV Infections*, Humans ; Male ; CD4-Positive T-Lymphocytes ; T-Lymphocyte Subsets ; Cryopreservation ; HLA-DR Antigens
مستخلص: Voluntary medical male circumcision (VMMC) reduces HIV acquisition by at least 60%, but the determinants of HIV susceptibility in foreskin tissues are incompletely understood. Flow cytometry is a powerful tool that helps us understand tissue immune defenses in mucosal tissue like the inner foreskin, but foreskin flow cytometry has only been validated using fresh tissue samples. This restricts immune analyses to timepoints immediately after surgical acquisition and hinders research in this area. We compared fresh analysis with whole tissue cryopreservation and later thawing and digestion to analyze CD4+ T cell populations relevant to HIV susceptibility (CCR5, CD25, CD127, CCR4, CXCR3, CCR6, CCR10, HLA-DR, and CD38). Eight foreskin samples from HIV-negative males aged >18 years were collected after VMMC. For each sample, half the foreskin was immediately cryopreserved for later digestion and flow cytometry analysis, while the remaining tissues were analyzed fresh. We demonstrate no significant impact of cryopreservation on CD4+ T cell expression of CD25, CCR4, CCR6, HLA-DR, CCR10, or CD127. Although expression levels of CCR5, CD38, and CXCR3 were increased after cryopreservation, the relative ranking of participants was retained. In conclusion, cryopreserved foreskin tissues may be suitable for subsequent digestion and flow cytometry phenotyping of HIV-susceptible T cell populations.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Almomani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
References: Cytometry A. 2019 Jun;95(6):647-654. (PMID: 30714682)
J Biol Methods. 2020 Apr 13;7(2):e131. (PMID: 32313815)
PLoS One. 2010 Dec 16;5(12):e15552. (PMID: 21179493)
Cytotherapy. 2020 May;22(5):291-300. (PMID: 32220549)
AIDS. 2009 Jan 28;23(3):319-28. (PMID: 19114867)
Mucosal Immunol. 2010 Jul;3(4):410-8. (PMID: 20410876)
J Immunol Methods. 2003 Jul;278(1-2):145-55. (PMID: 12957403)
Mucosal Immunol. 2012 Mar;5(2):121-8. (PMID: 22089029)
BMC Infect Dis. 2006 Nov 30;6:172. (PMID: 17137513)
PLoS Pathog. 2011 Jun;7(6):e1002100. (PMID: 21738469)
Transl Androl Urol. 2017 Apr;6(2):149-157. (PMID: 28540221)
AIDS Res Ther. 2017 Sep 12;14(1):49. (PMID: 28893286)
PLoS Pathog. 2016 Nov 29;12(11):e1006025. (PMID: 27898732)
JCI Insight. 2021 Apr 22;6(8):. (PMID: 33884964)
Am J Reprod Immunol. 2019 Jul;82(1):e13143. (PMID: 31081958)
J Virol. 2011 Oct;85(19):10189-200. (PMID: 21813616)
N Engl J Med. 2009 Mar 26;360(13):1298-309. (PMID: 19321868)
BMC Infect Dis. 2007 Mar 13;7:16. (PMID: 17355625)
J Immunol. 2010 Feb 1;184(3):1604-16. (PMID: 20042588)
Am J Obstet Gynecol. 2009 Jan;200(1):42.e1-7. (PMID: 18976733)
Lancet. 2007 Feb 24;369(9562):657-66. (PMID: 17321311)
Curr HIV/AIDS Rep. 2022 Dec;19(6):484-490. (PMID: 36308579)
Clin Exp Immunol. 1994 Mar;95(3):436-41. (PMID: 7907956)
Nat Immunol. 2009 Aug;10(8):864-71. (PMID: 19578368)
Mucosal Immunol. 2010 Sep;3(5):506-22. (PMID: 20571487)
Microbiome. 2022 Jan 18;10(1):7. (PMID: 35042542)
PLoS Pathog. 2014 Oct 02;10(10):e1004416. (PMID: 25275513)
PLoS One. 2018 Jul 30;13(7):e0200653. (PMID: 30059507)
Mucosal Immunol. 2020 Jan;13(1):118-127. (PMID: 31619762)
Mucosal Immunol. 2018 Jan;11(1):158-171. (PMID: 28443609)
Front Immunol. 2021 Nov 29;12:765667. (PMID: 34912338)
PLoS Pathog. 2022 Jan 4;18(1):e1009948. (PMID: 34982799)
Mucosal Immunol. 2014 May;7(3):634-44. (PMID: 24150258)
J Immunol. 2002 Jan 1;168(1):65-72. (PMID: 11751947)
PLoS Negl Trop Dis. 2015 Sep 03;9(9):e0004067. (PMID: 26335139)
PLoS Pathog. 2015 Mar 06;11(3):e1004729. (PMID: 25748093)
معلومات مُعتمدة: R01 AI128779 United States AI NIAID NIH HHS; R01 DK131936 United States DK NIDDK NIH HHS
المشرفين على المادة: 0 (HLA-DR Antigens)
تواريخ الأحداث: Date Created: 20240301 Date Completed: 20240304 Latest Revision: 20240309
رمز التحديث: 20240309
مُعرف محوري في PubMed: PMC10906856
DOI: 10.1371/journal.pone.0297884
PMID: 38427640
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0297884