دورية أكاديمية
The mechanism of encapsulating curcumin into oleosomes (Lipid Droplets).
| العنوان: | The mechanism of encapsulating curcumin into oleosomes (Lipid Droplets). |
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| المؤلفون: | Vardar US; Biobased Chemistry and Technology, Wageningen University and Research, Bornse Weilanden 9, Wageningen 6708 WG, the Netherlands., Bitter JH; Biobased Chemistry and Technology, Wageningen University and Research, Bornse Weilanden 9, Wageningen 6708 WG, the Netherlands., Nikiforidis CV; Biobased Chemistry and Technology, Wageningen University and Research, Bornse Weilanden 9, Wageningen 6708 WG, the Netherlands. Electronic address: costas.nikiforidis@wur.nl. |
| المصدر: | Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2024 Apr; Vol. 236, pp. 113819. Date of Electronic Publication: 2024 Feb 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9315133 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4367 (Electronic) Linking ISSN: 09277765 NLM ISO Abbreviation: Colloids Surf B Biointerfaces Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, c1993- |
| مواضيع طبية MeSH: | Lipid Droplets* , Curcumin*, Fluorescence |
| مستخلص: | Organisms have evolved intracellular micron-sized lipid droplets to carry and protect lipids and hydrophobic minor compounds in the hydrophilic environment of cells. These droplets can be utilized as carriers of hydrophobic therapeutics by taking advantage of their biological functions. Here, we focus on the potential of plant-derived lipid droplets, known as oleosomes, as carriers for hydrophobic therapeutics, such as curcumin. By spectroscopy and confocal microscopy, we demonstrate that the oleosome membrane is permeable to hydrophobic curcumin molecules. Fluorescence recovery after photobleaching shows rapid curcumin diffusion towards oleosomes, with a diffusion time in the range of seconds. Following this, quenching probes and dilatational rheology reveal that part of the loaded curcumin molecules can accumulate at the oleosome interface, and the rest settle in the inner core. Our findings shed light on the loading mechanism of the plant-derived lipid droplets and underscore the significance of molecular localization for understanding the mechanism. This work not only enhances the understanding of the loading process but also shows potential for oleosomes use as lipid carriers. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Curcumin; Lipid droplets; Lipophilic; Oil bodies; Rapeseeds; Therapeutics |
| المشرفين على المادة: | IT942ZTH98 (Curcumin) |
| تواريخ الأحداث: | Date Created: 20240301 Date Completed: 20240320 Latest Revision: 20240320 |
| رمز التحديث: | 20240320 |
| DOI: | 10.1016/j.colsurfb.2024.113819 |
| PMID: | 38428208 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4367 |
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| DOI: | 10.1016/j.colsurfb.2024.113819 |