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Silencing NlFAR7 destroyed the pore canals and related structures of the brown planthopper.

التفاصيل البيبلوغرافية
العنوان: Silencing NlFAR7 destroyed the pore canals and related structures of the brown planthopper.
المؤلفون: Cui YL; Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, China., Guo JS; Center of Cryo-Electron Microscopy, Zhejiang University School of Medicine, Hangzhou, China., Zhang CX; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo, China., Yu XP; Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, China., Li DT; Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, China.
المصدر: Insect molecular biology [Insect Mol Biol] 2024 Aug; Vol. 33 (4), pp. 350-361. Date of Electronic Publication: 2024 Mar 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Scientific For The Royal Entomological Society Country of Publication: England NLM ID: 9303579 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2583 (Electronic) Linking ISSN: 09621075 NLM ISO Abbreviation: Insect Mol Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford : Blackwell Scientific For The Royal Entomological Society
Original Publication: Oxford : Published for the Royal Entomological Society by Blackwell Scientific Publications, c1992-
مواضيع طبية MeSH: Hemiptera*/genetics , Hemiptera*/metabolism , Insect Proteins*/metabolism , Insect Proteins*/genetics , Insect Proteins*/chemistry, Animals ; RNA Interference ; Aldehyde Oxidoreductases/metabolism ; Aldehyde Oxidoreductases/genetics ; Microscopy, Electron, Scanning
مستخلص: Fatty acyl-CoA reductase (FAR) is one of the key enzymes, which catalyses the conversion of fatty acyl-CoA to the corresponding alcohols. Among the FAR family members in the brown planthopper (Nilaparvata lugens), NlFAR7 plays a pivotal role in both the synthesis of cuticular hydrocarbons and the waterproofing of the cuticle. However, the precise mechanism by which NlFAR7 influences the formation of the cuticle structure in N. lugens remains unclear. Therefore, this paper aims to investigate the impact of NlFAR7 through RNA interference, transmission electron microscope, focused ion beam scanning electron microscopy (FIB-SEM) and lipidomics analysis. FIB-SEM is employed to reconstruct the three-dimensional (3D) architecture of the pore canals and related cuticle structures in N. lugens subjected to dsNlFAR7 and dsGFP treatments, enabling a comprehensive assessment of changes in the cuticle structures. The results reveal a reduction in the thickness of the cuticle and disruptions in the spiral structure of pore canals, accompanied by widened base and middle diameters. Furthermore, the lipidomics comparison analysis between dsNlFAR7- and dsGFP-treated N. lugens demonstrated that there were 25 metabolites involved in cuticular lipid layer synthesis, including 7 triacylglycerols (TGs), 5 phosphatidylcholines (PCs), 3 phosphatidylethanolamines (PEs) and 2 diacylglycerols (DGs) decreased, and 4 triacylglycerols (TGs) and 4 PEs increased. In conclusion, silencing NlFAR7 disrupts the synthesis of overall lipids and destroys the cuticular pore canals and related structures, thereby disrupting the secretion of cuticular lipids, thus affecting the cuticular waterproofing of N. lugens. These findings give significant attention with reference to further biochemical researches on the substrate specificity of FAR protein, and the molecular regulation mechanisms during N. lugens life cycle.
(© 2024 Royal Entomological Society.)
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معلومات مُعتمدة: 32001895 National Natural Science Foundation of China; 32372526 National Natural Science Foundation of China; U21A20223 National Nature Science Foundation of China Regional Innovation and Development Joint Fund Key Support Project; LY23C140007 Natural Science Foundation of Zhejiang Province
فهرسة مساهمة: Keywords: 3D reconstruction; Nilaparvata lugens; cuticle; fatty acyl‐CoA reductase; lipidomics; pore canal
المشرفين على المادة: 0 (Insect Proteins)
EC 1.2.- (Aldehyde Oxidoreductases)
EC 1.2.1.42 (hexadecanal dehydrogenase (acylating))
تواريخ الأحداث: Date Created: 20240302 Date Completed: 20240709 Latest Revision: 20240731
رمز التحديث: 20240801
DOI: 10.1111/imb.12903
PMID: 38430546
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-2583
DOI:10.1111/imb.12903