دورية أكاديمية
Lower (1,3)-beta-d-glucan sensitivity and in vitro levels in Candida auris and Candida parapsilosis strains.
| العنوان: | Lower (1,3)-beta-d-glucan sensitivity and in vitro levels in Candida auris and Candida parapsilosis strains. |
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| المؤلفون: | Mikulska M; Division of Infectious Diseases, Department of Health Sciences (DISSAL), University of Genova, Genova, Italy; Division of Infectious Diseases, IRCCS Ospedale Policlinico San Martino, Genova, Italy. Electronic address: m.mikulska@unige.it., Ullah N; Division of Infectious Diseases, Department of Health Sciences (DISSAL), University of Genova, Genova, Italy., Magnasco L; Division of Infectious Diseases, Department of Health Sciences (DISSAL), University of Genova, Genova, Italy., Codda G; Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy., Bartalucci C; Division of Infectious Diseases, Department of Health Sciences (DISSAL), University of Genova, Genova, Italy; Division of Infectious Diseases, IRCCS Ospedale Policlinico San Martino, Genova, Italy., Miletich F; Division of Infectious Diseases, Department of Health Sciences (DISSAL), University of Genova, Genova, Italy; Division of Infectious Diseases, IRCCS Ospedale Policlinico San Martino, Genova, Italy., Sepulcri C; Division of Infectious Diseases, Department of Health Sciences (DISSAL), University of Genova, Genova, Italy; Division of Infectious Diseases, IRCCS Ospedale Policlinico San Martino, Genova, Italy., Willison E; Microbiology Unit, San Martino Policlinico Hospital - IRCCS for Oncology and Neurosciences, Genoa, Italy., Vena A; Division of Infectious Diseases, Department of Health Sciences (DISSAL), University of Genova, Genova, Italy; Division of Infectious Diseases, IRCCS Ospedale Policlinico San Martino, Genova, Italy., Giacobbe DR; Division of Infectious Diseases, Department of Health Sciences (DISSAL), University of Genova, Genova, Italy; Division of Infectious Diseases, IRCCS Ospedale Policlinico San Martino, Genova, Italy., di Pilato V; Microbiology Unit, San Martino Policlinico Hospital - IRCCS for Oncology and Neurosciences, Genoa, Italy., Robba C; Anesthesia and Intensive Care, Ospedale Policlinico San Martino, IRCCS per l'Oncologia e le Neuroscienze, Genoa, Italy., Ball L; Anesthesia and Intensive Care, Ospedale Policlinico San Martino, IRCCS per l'Oncologia e le Neuroscienze, Genoa, Italy., Marchese A; Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy; Microbiology Unit, San Martino Policlinico Hospital - IRCCS for Oncology and Neurosciences, Genoa, Italy., Bassetti M; Division of Infectious Diseases, Department of Health Sciences (DISSAL), University of Genova, Genova, Italy; Division of Infectious Diseases, IRCCS Ospedale Policlinico San Martino, Genova, Italy. |
| المصدر: | Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect] 2024 Jun; Vol. 30 (6), pp. 822-827. Date of Electronic Publication: 2024 Feb 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 9516420 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-0691 (Electronic) Linking ISSN: 1198743X NLM ISO Abbreviation: Clin Microbiol Infect Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Elsevier Original Publication: Paris : Decker Europe, c1995- |
| مواضيع طبية MeSH: | beta-Glucans*/blood , Candidemia*/microbiology , Candidemia*/diagnosis , Candidemia*/blood , Candida parapsilosis*/isolation & purification, Humans ; Male ; Female ; Middle Aged ; Candida auris ; Aged ; Proteoglycans ; Candida albicans/isolation & purification ; Sensitivity and Specificity ; Adult ; Microbial Sensitivity Tests ; Candida/classification ; Candida/isolation & purification ; Antifungal Agents/pharmacology ; Aged, 80 and over |
| مستخلص: | Objectives: The serum (1,3)-beta-d-glucan (BDG) assay gives quicker results and has higher sensitivity than blood cultures, therefore it is advised for early diagnosis of invasive candidemia and/or discontinuation of empirical therapy. Its sensitivity may depend on different factors. The aim of our study was to analyse the in vitro and in vivo BDG levels in clinical isolates of three species of Candida responsible for candidemia. Methods: C. albicans, C. parapsilosis, and C. auris strains were collected from blood cultures of patients who had a concurrent (-1 to +3 days) serum BDG test (Fungitell assay). Supernatants of all strains were tested in quadruplicate for BDG levels. Results: Twenty-two C. auris, 14 C. albicans, and ten C. parapsilosis strains were included. The median BDG levels in supernatants were 463 pg/mL (interquartile range [IQR] 379-648) for C. auris, 1080 pg/mL (IQR 830-1276) for C. albicans, and 755 pg/mL (IQR 511-930) for C. parapsilosis, with the significant difference among the species (p < 0.0001). Median serum BDG levels (IQR) were significantly lower in case C. auris and C. parapsilosis vs. C. albicans (p < 0.0001), respectively, 50 pg/mL (IQR 15-161) and 57 pg/mL (IQR 18-332), vs. 372 pg/mL (IQR 102-520). Sensitivity of serum BDG was 39% (95% confidence interval [CI], 18-64) in case of C. auris, 30% (95% CI, 8-65) C. parapsilosis and 78% (95% CI, 49-94) C. albicans candidemia. Discussion: In our centre C. auris and C. parapsilosis strains have lower BDG content as compared with C. albicans, with a potential impact on serum BDG performance for the diagnosis of candidemia. (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Candida albicans; Candida auris; Candida parapsilosis; Candidemia; De-escalation; Glucan |
| المشرفين على المادة: | 0 (beta-Glucans) 0 (Proteoglycans) 3X48A86C8K (polysaccharide-K) 0 (Antifungal Agents) |
| تواريخ الأحداث: | Date Created: 20240302 Date Completed: 20240527 Latest Revision: 20240527 |
| رمز التحديث: | 20240528 |
| DOI: | 10.1016/j.cmi.2024.02.012 |
| PMID: | 38431255 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1469-0691 |
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| DOI: | 10.1016/j.cmi.2024.02.012 |