دورية أكاديمية
A synthetic flavonoid derivate in the plasma membrane transforms the voltage-clamp fluorometry signal of CiHv1.
| العنوان: | A synthetic flavonoid derivate in the plasma membrane transforms the voltage-clamp fluorometry signal of CiHv1. |
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| المؤلفون: | Pethő Z; Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Hungary.; Institut für Physiologie II, University of Münster, Germany., Pajtás D; Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Hungary., Piga M; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ljubljana, Slovenia., Magyar Z; Department of Physiology, Faculty of Medicine, University of Debrecen, Hungary., Zakany F; Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Hungary., Kovacs T; Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Hungary., Zidar N; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ljubljana, Slovenia., Panyi G; Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Hungary., Varga Z; Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Hungary., Papp F; Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Hungary. |
| المصدر: | The FEBS journal [FEBS J] 2024 Jun; Vol. 291 (11), pp. 2354-2371. Date of Electronic Publication: 2024 Mar 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published by Blackwell Pub. on behalf of the Federation of European Biochemical Societies Country of Publication: England NLM ID: 101229646 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1742-4658 (Electronic) Linking ISSN: 1742464X NLM ISO Abbreviation: FEBS J Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford, UK : Published by Blackwell Pub. on behalf of the Federation of European Biochemical Societies, c2005- |
| مواضيع طبية MeSH: | Cell Membrane*/metabolism , Cell Membrane*/chemistry , Fluorometry*/methods , Patch-Clamp Techniques* , Ciona intestinalis*/metabolism , Ciona intestinalis*/chemistry , Ciona intestinalis*/genetics , Phenylalanine*/chemistry , Phenylalanine*/analogs & derivatives, Animals ; Oocytes/metabolism ; Flavonoids/chemistry ; Flavonoids/pharmacology ; Xenopus laevis ; Ion Channels/metabolism ; Ion Channels/chemistry ; Fluorescent Dyes/chemistry ; Humans |
| مستخلص: | Voltage-clamp fluorometry (VCF) enables the study of voltage-sensitive proteins through fluorescent labeling accompanied by ionic current measurements for voltage-gated ion channels. The heterogeneity of the fluorescent signal represents a significant challenge in VCF. The VCF signal depends on where the cysteine mutation is incorporated, making it difficult to compare data among different mutations and different studies and standardize their interpretation. We have recently shown that the VCF signal originates from quenching amino acids in the vicinity of the attached fluorophores, together with the effect of the lipid microenvironment. Based on these, we performed experiments to test the hypothesis that the VCF signal could be altered by amphiphilic quenching molecules in the cell membrane. Here we show that a phenylalanine-conjugated flavonoid (4-oxo-2-phenyl-4H-chromene-7-yl)-phenylalanine, (later Oxophench) has potent effects on the VCF signals of the Ciona intestinalis H (© 2024 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.) |
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| معلومات مُعتمدة: | P1-0208 Slovenian Research Agency; BO/00355/21/8 János Bolyai Research Scholarship of the Hungarian Academy of Sciences; 2019-00059 2019-2.1.11-TÉT; 1G3DBKB0BFPF247 OTKA Bridging Fund; 132906 OTKA; ÚNKP-22-5-DE-420 New National Excellence Program of the Ministry for Innovation and Technology |
| فهرسة مساهمة: | Keywords: CiHv1; flavonoid derivate; fluorescence‐quenching; membrane fluidity; voltage‐clamp fluorometry |
| المشرفين على المادة: | 47E5O17Y3R (Phenylalanine) 0 (Flavonoids) 0 (Ion Channels) 0 (Fluorescent Dyes) |
| تواريخ الأحداث: | Date Created: 20240303 Date Completed: 20240605 Latest Revision: 20240605 |
| رمز التحديث: | 20240605 |
| DOI: | 10.1111/febs.17105 |
| PMID: | 38431775 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1742-4658 |
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| DOI: | 10.1111/febs.17105 |