دورية أكاديمية
أعرض في EDS

Role of steroid hormones in the maintenance of focal adhesions in bovine oviductal epithelial cells.

التفاصيل البيبلوغرافية
العنوان: Role of steroid hormones in the maintenance of focal adhesions in bovine oviductal epithelial cells.
المؤلفون: Vella MA; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, T4000ILI San Miguel de Tucumán, Tucumán, Argentina., García DC; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, T4000ILI San Miguel de Tucumán, Tucumán, Argentina; Instituto de Ciencias Químicas (ICQ), Facultad de Agronomía y Agroindustrias (FAyA), Universidad Nacional de Santiago del Estero (UNSE)-CONICET. RN 9 Km 1125. Villa El Zanjón. CP4206 Santiago del Estero, Argentina., De Boeck M; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, T4000ILI San Miguel de Tucumán, Tucumán, Argentina., Valdecantos PA; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, T4000ILI San Miguel de Tucumán, Tucumán, Argentina., Roldán-Olarte M; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, T4000ILI San Miguel de Tucumán, Tucumán, Argentina. Electronic address: eugenia.roldanolarte@fbqf.unt.edu.ar.
المصدر: Domestic animal endocrinology [Domest Anim Endocrinol] 2024 Jul; Vol. 88, pp. 106839. Date of Electronic Publication: 2024 Feb 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 8505191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0054 (Electronic) Linking ISSN: 07397240 NLM ISO Abbreviation: Domest Anim Endocrinol Subsets: MEDLINE
أسماء مطبوعة: Publication: Stoneham, Ma : Elsevier
Original Publication: [Auburn, Ala.] : Domendo, Inc., c1984-
مواضيع طبية MeSH: Estradiol*/pharmacology , Focal Adhesions* , Progesterone*/pharmacology , Progesterone*/metabolism , Epithelial Cells*/physiology , Fallopian Tubes*/physiology , Fallopian Tubes*/cytology, Animals ; Female ; Cattle ; Paxillin/metabolism ; Paxillin/genetics ; Cell Movement ; Estrous Cycle/physiology ; Cells, Cultured ; Focal Adhesion Protein-Tyrosine Kinases/metabolism ; Gene Expression Regulation ; Oviducts/physiology
مستخلص: The oviduct, the organ of the female reproductive system where fertilization and early embryonic development occur, provides an optimal environment for the final maturation of oocytes, storage, and sperm capacitation and transport of gametes and embryos. During the estrous cycle, the oviduct is affected by ovarian sex hormones, resulting in changes aimed at maintaining an appropriate microenvironment. Normal cell migration is tightly regulated, its role being essential for the development and maintenance of organ and tissue functions as well as for regeneration following injury. Due to their involvement in focal contact formations, focal adhesion kinase (PTK2) and paxillin (PXN) are key proteins in the study of cell migration and adhesion. The objective of this work was to compare the expression of PTK2 and PXN in oviductal cells along the estrous cycle and to determine if their expression is regulated by the presence of 17-β estradiol (E 2 ) and/or progesterone (P 4 ). No transcripts of PTK2 or of PXN were detected in cells corresponding to the luteal phase. Additionally, hormonal stimulation experiments on bovine oviductal cell cultures (BOECs) were carried out, where P 4 inhibited the expression of both genes. Migration assays demonstrated that P 4 reduced BOECs migration capacity. P 4 treatment also reduced cell adhesion, while E 2 increased the number of adhered cells. In conclusion, the presence of E 2 and P 4 regulates the expression of genes involved in the formation of focal contacts and modifies the migration and adhesion of BOECs. Understanding the effect of steroid hormones on BOECs is critical to grasp the impact of steroid control on oviductal function and its contribution to establishing successful pregnancies.
Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interests that could be perceived as prejudicing the impartiality of the research reported.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: 17–β-estradiol; Adhesion; Focal adhesion kinase; Migration; Paxillin; Progesterone
المشرفين على المادة: 4TI98Z838E (Estradiol)
4G7DS2Q64Y (Progesterone)
0 (Paxillin)
EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
تواريخ الأحداث: Date Created: 20240303 Date Completed: 20240605 Latest Revision: 20240605
رمز التحديث: 20240606
DOI: 10.1016/j.domaniend.2024.106839
PMID: 38433026
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0054
DOI:10.1016/j.domaniend.2024.106839