دورية أكاديمية
أعرض في EDS

Genetic characterisation of multidrug-resistant Salmonella enterica serotypes isolated from poultry in Cairo, Egypt.

التفاصيل البيبلوغرافية
العنوان: Genetic characterisation of multidrug-resistant Salmonella enterica serotypes isolated from poultry in Cairo, Egypt.
المؤلفون: Abdel-Maksoud M; Global Disease Detection and Response Program, US Naval Medical Research Unit, Egypt., Abdel-Khalek R; Bacterial and Parasitic Disease Research Program, US Naval Medical Research Unit, Egypt., El-Gendy A; Bacterial and Parasitic Disease Research Program, US Naval Medical Research Unit, Egypt., Gamal RF; Faculty of Agriculture, Ain Shams University, Department of Microbiology, Egypt., Abdelhady HM; Faculty of Agriculture, Ain Shams University, Department of Microbiology, Egypt., House BL; Global Disease Detection and Response Program, US Naval Medical Research Unit, Egypt.
المصدر: African journal of laboratory medicine [Afr J Lab Med] 2015 May 14; Vol. 4 (1), pp. 1-7. Date of Electronic Publication: 2015 May 14 (Print Publication: 2015).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: AOSIS OpenJournals Country of Publication: South Africa NLM ID: 101603205 Publication Model: eCollection Cited Medium: Print ISSN: 2225-2002 (Print) Linking ISSN: 22252002 NLM ISO Abbreviation: Afr J Lab Med Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Durbanville, South Africa : AOSIS OpenJournals, [2012]-
مستخلص: Background: Food-borne diseases pose serious health problems, affecting public health and economic development worldwide.
Methods: Salmonella was isolated from samples of chicken parts, skin samples of whole chicken carcasses, raw egg yolks, eggshells and chicken faeces. Resulting isolates were characterised by serogrouping, serotyping, antimicrobial susceptibility testing and detection of extended-spectrum β-lactamase (ESBL) production. Antibiotic resistance genes and integrons were identified by polymerase chain reaction (PCR).
Results: The detection rates of Salmonella were 60%, 64% and 62% in chicken parts, skin, and faeces, respectively, whereas the egg yolks and eggshells were uniformly negative. Salmonella Kentucky and S. Enteritidis serotypes comprised 43.6% and 2.6% of the isolates, respectively, whilst S. Typhimurium was absent. Variable resistance rates were observed against 16 antibiotics; 97% were resistant to sulfamethoxazole, 96% to nalidixic acid and tetracycline and 76% to ampicillin. Multidrug resistance was detected in 82% (64/78) of the isolates and ESBL production was detected in 8% (6/78). The β-lactamase bla TEM-1 gene was detected in 57.6% and bla SHV-1 in 6.8% of the isolates, whilst the bla OXA gene was absent. The sul1 gene was detected in 97.3% and the sul2 gene in 5.3% of the isolates. Sixty-four of the 78 isolates (82%) were positive for the integrase gene ( int I ) from class 1 integrons, whilst int II was absent.
Conclusion: This study reveals the presence of an alarming number of multidrug-resistant Salmonella isolates in the local poultry markets in Cairo. The high levels of drug resistance suggest an emerging problem that could impact negatively on efforts to prevent and treat poultry and poultry-transmitted human diseases in Egypt.
Competing Interests: The authors declare that they have no financial or personal relationship(s) that may have inappropriately influenced them in writing this article.
(© 2015. The Authors.)
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تواريخ الأحداث: Date Created: 20240305 Latest Revision: 20240306
رمز التحديث: 20240306
مُعرف محوري في PubMed: PMC10911655
DOI: 10.4102/ajlm.v4i1.158
PMID: 38440309
قاعدة البيانات: MEDLINE
الوصف
تدمد:2225-2002
DOI:10.4102/ajlm.v4i1.158