دورية أكاديمية
Role of induced nitric oxide synthases in orofacial nociception/discomfort after dental tooth bleaching with hydrogen peroxide.
| العنوان: | Role of induced nitric oxide synthases in orofacial nociception/discomfort after dental tooth bleaching with hydrogen peroxide. |
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| المؤلفون: | Pinto MR; Department of Dentistry, Unichristus, Fortaleza, Ceará, Brazil., da Silva Medeiros KI; Department of Dentistry, Unichristus, Fortaleza, Ceará, Brazil., Maia LM; Department of Dentistry, Unichristus, Fortaleza, Ceará, Brazil., Coelho AA; Department of Dentistry, Unichristus, Fortaleza, Ceará, Brazil., Negreiros Nunes Alves AP; Department of Dental Clinic, Division of Oral Pathology, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil., Caetano CFF; Department of Dentistry, Unichristus, Fortaleza, Ceará, Brazil., Mesquita KC; Department of Dentistry, Unichristus, Fortaleza, Ceará, Brazil., de Barros Silva PG; Department of Dentistry, Unichristus, Fortaleza, Ceará, Brazil. Electronic address: paulo_goberlanio@yahoo.com.br., Sousa FB; Department of Dentistry, Unichristus, Fortaleza, Ceará, Brazil. |
| المصدر: | Archives of oral biology [Arch Oral Biol] 2024 May; Vol. 161, pp. 105937. Date of Electronic Publication: 2024 Mar 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 0116711 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1506 (Electronic) Linking ISSN: 00039969 NLM ISO Abbreviation: Arch Oral Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Pergamon Press Original Publication: Oxford, New York [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Tooth Bleaching* , Tooth Bleaching Agents* , Guanidines*, Rats ; Animals ; Hydrogen Peroxide/pharmacology ; Nociception ; Nitric Oxide ; Rats, Wistar ; Nitric Oxide Synthase |
| مستخلص: | Objective: To evaluate the role of induced nitric oxide synthase (iNOS) in nociception/orofacial discomfort in rats submitted to tooth whitening with hydrogen peroxide (H₂O₂). Design: Wistar rats were divided into three groups (n = 24/group): a sham group not submitted to whitening treatment, a saline group submitted to whitening treatment, and a test group submitted to whitening treatment and blockade of iNOS with aminoguanidine 50 mg/kg/day. After 24 and 48 h, and 7 days, the animals were euthanized to collect trigeminal ganglia and maxillae to histomorphometric analysis (size of neuronal bodies and percentage of pulp area filled by vessels) and behavior/nociception (Grimace scales, scratching and biting counting, weight loss and nociception assay). ANOVA-1- or - 2-way tests were used (p < 0.05, GraphPadPrism 5.0). Results: The aminoguanidine-treated group showed a reduction in nociceptive threshold in the masseteric region (p < 0.001), Grimace scale scores (p < 0.001), number of scratching (p = 0.011) and body mass loss (p = 0.007). After 24 and 48 h of tooth bleaching, the saline group showed a significant increase in the mean area of the blood vessels (p = 0.020) and iNOS immunostaining in odontoblasts (p = 0.002) and non-odontoblasts cells (p = 0.025). Aminoguanidine reversed both increases. Tooth bleaching reduced the mean area of neuronal bodies, and aminoguanidine significantly reversed it (p = 0.019), but an increase in GFAP immunostaining in neuronal bodies did not reduce after seven-days or after aminoguanidine treatment (p = 0.003). Conclusion: iNOS blockage by aminoguanidine plays an important role in nociception and orofacial discomfort by control of inflammation in dental pulp after tooth bleaching with hydrogen peroxide (H₂O₂) 35%. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Pain. Tooth Bleaching. Aminoguanidine. Nitric oxide |
| المشرفين على المادة: | BBX060AN9V (Hydrogen Peroxide) SCQ4EZQ113 (pimagedine) 31C4KY9ESH (Nitric Oxide) EC 1.14.13.39 (Nitric Oxide Synthase) 0 (Tooth Bleaching Agents) 0 (Guanidines) |
| تواريخ الأحداث: | Date Created: 20240305 Date Completed: 20240315 Latest Revision: 20240315 |
| رمز التحديث: | 20240315 |
| DOI: | 10.1016/j.archoralbio.2024.105937 |
| PMID: | 38442471 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1879-1506 |
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| DOI: | 10.1016/j.archoralbio.2024.105937 |