دورية أكاديمية
أعرض في EDS

Characterization of rumen microbiome and immune genes expression of crossbred beef steers with divergent residual feed intake phenotypes.

التفاصيل البيبلوغرافية
العنوان: Characterization of rumen microbiome and immune genes expression of crossbred beef steers with divergent residual feed intake phenotypes.
المؤلفون: Taiwo G; Division of Animal and Nutritional Science, West Virginia University, 26505, Morgantown, WV, USA., Morenikeji OB; Division of Biological and Health Sciences, University of Pittsburgh at Bradford, 300 Campus Drive, 16701, Bradford, PA, USA. obm3@pitt.edu., Idowu M; Division of Animal and Nutritional Science, West Virginia University, 26505, Morgantown, WV, USA., Sidney T; Division of Animal and Nutritional Science, West Virginia University, 26505, Morgantown, WV, USA., Adekunle A; Division of Animal and Nutritional Science, West Virginia University, 26505, Morgantown, WV, USA., Cervantes AP; Agricultural Research Station, Fort Valley State University, Fort Valley, GA, USA., Peters S; Department of Animal Science, Berry College, Mount Berry, GA, USA., Ogunade IM; Division of Animal and Nutritional Science, West Virginia University, 26505, Morgantown, WV, USA. Ibukun.ogunade@mail.wvu.edu.
المصدر: BMC genomics [BMC Genomics] 2024 Mar 05; Vol. 25 (1), pp. 245. Date of Electronic Publication: 2024 Mar 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2000-
مواضيع طبية MeSH: Rumen* , Vascular Endothelial Growth Factor A*, Animals ; Cattle ; Phenotype ; Bacteroidetes ; Eating ; RNA, Messenger
مستخلص: We investigated whole blood and hepatic mRNA expressions of immune genes and rumen microbiome of crossbred beef steers with divergent residual feed intake phenotype to identify relevant biological processes underpinning feed efficiency in beef cattle. Low-RFI beef steers (n = 20; RFI = - 1.83 kg/d) and high-RFI beef steers (n = 20; RFI = + 2.12 kg/d) were identified from a group of 108 growing crossbred beef steers (average BW = 282 ± 30.4 kg) fed a high-forage total mixed ration after a 70-d performance testing period. At the end of the 70-d testing period, liver biopsies and blood samples were collected for total RNA extraction and cDNA synthesis. Rumen fluid samples were also collected for analysis of the rumen microbial community. The mRNA expression of 84 genes related to innate and adaptive immunity was analyzed using pathway-focused PCR-based arrays. Differentially expressed genes were determined using P-value ≤ 0.05 and fold change (FC) ≥ 1.5 (in whole blood) or ≥ 2.0 (in the liver). Gene ontology analysis of the differentially expressed genes revealed that pathways related to pattern recognition receptor activity, positive regulation of phagocytosis, positive regulation of vitamin metabolic process, vascular endothelial growth factor production, positive regulation of epithelial tube formation and T-helper cell differentiation were significantly enriched (FDR < 0.05) in low-RFI steers. In the rumen, the relative abundance of PeH15, Arthrobacter, Moryella, Weissella, and Muribaculaceae was enriched in low-RFI steers, while Methanobrevibacter, Bacteroidales_BS11_gut_group, Bacteroides and Clostridium_sensu_stricto_1 were reduced. In conclusion, our study found that low-RFI beef steers exhibit increased mRNA expression of genes related to immune cell functions in whole blood and liver tissues, specifically those involved in pathogen recognition and phagocytosis regulation. Additionally, these low-RFI steers showed differences in the relative abundance of some microbial taxa which may partially account for their improved feed efficiency compared to high-RFI steers.
(© 2024. The Author(s).)
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معلومات مُعتمدة: W-3010 West Virginia University Experimental Station in support of the U.S. Department of Agriculture hatch multi-state regional project
فهرسة مساهمة: Keywords: Immunity; RFI; Rumen microbiota
المشرفين على المادة: 0 (Vascular Endothelial Growth Factor A)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20240305 Date Completed: 20240307 Latest Revision: 20240308
رمز التحديث: 20240308
مُعرف محوري في PubMed: PMC10913640
DOI: 10.1186/s12864-024-10150-3
PMID: 38443809
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2164
DOI:10.1186/s12864-024-10150-3