دورية أكاديمية
[Deficiency of cathepsin K improves ischemic angiogenesis in high-fat diet fed mice].
| العنوان: | [Deficiency of cathepsin K improves ischemic angiogenesis in high-fat diet fed mice]. |
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| المؤلفون: | Wang XY; Department of Basic Medicine, Zhejiang Chinese Medical University, Hangzhou 310000, China.; Department of Medicine, Jiaxing University, Jiaxing 314000, China., Wang X; Department of Medicine, Jiaxing University, Jiaxing 314000, China., He ZJ; Department of Medicine, Jiaxing University, Jiaxing 314000, China., Chen SP; Department of Medicine, Jiaxing University, Jiaxing 314000, China., Li WJ; Department of Medicine, Jiaxing University, Jiaxing 314000, China., Cui RS; Department of Medicine, Jiaxing University, Jiaxing 314000, China., Jiang HY; Department of Medicine, Jiaxing University, Jiaxing 314000, China.; Department of Physiology, Yanbian University, Yanji 133000, China. hyjiang7689@zjxu.edu.cn. |
| المصدر: | Sheng li xue bao : [Acta physiologica Sinica] [Sheng Li Xue Bao] 2024 Feb 25; Vol. 76 (1), pp. 45-51. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Science Press Country of Publication: China NLM ID: 20730130R Publication Model: Print Cited Medium: Internet ISSN: 0371-0874 (Print) Linking ISSN: 03710874 NLM ISO Abbreviation: Sheng Li Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Science Press Original Publication: Beiping, 1953- |
| مواضيع طبية MeSH: | Diet, High-Fat*/adverse effects , Vascular Endothelial Growth Factor A*/genetics, Animals ; Mice ; Angiogenesis ; Cathepsin K ; Proto-Oncogene Proteins c-akt/genetics |
| مستخلص: | The present study aims to investigate the effect of cathepsin K (CatK) on ischemic angiogenesis in high-fat diet fed mice. The mice were subjected to unilateral hindlimb ischemic surgery, and the ischemic blood flow was measured with a laser Doppler blood flow imager. Immunohistochemical staining was used to observe the quantity of new capillaries in the ischemic lower extremity, and Western blot was used to detect the expression of insulin receptor substrate-1 (IRS-1), p-Akt, Akt and vascular endothelial growth factor (VEGF). Firstly, the effect of high-fat diet on ischemic angiogenesis was observed in wild-type mice, which were randomly divided into control group and high-fat diet group and were fed with normal diet or 60% high-fat diet respectively for 16 weeks. The results showed the body weight and the plasma CatK concentration of the high-fat diet group was significantly increased compared with the control group (P < 0.05), and the blood flow recovery of the high-fat diet group was significantly lower than control group (P < 0.05). Then, wild-type and CatK knock out (CatK -/- ) mice were both fed with high-fat diet to further observe the effect and mechanism of CatK on ischemic angiogenesis under high-fat diet. The results showed that the blood flow recovery in the CatK -/- group was significantly greater than the wild-type group, and the number of CD31 positive cells was significantly increased (P < 0.05). At the same time, the protein expression levels of IRS-1, p-Akt and VEGF in the ischemic skeletal muscle were significantly increased in the CatK -/- group compared with the wild-type group (P < 0.05). These results suggest that the deficiency of CatK improves ischemic angiogenesis in high-fat diet fed mice through IRS-1-Akt-VEGF signaling pathway. |
| المشرفين على المادة: | EC 3.4.22.38 (Cathepsin K) EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) 0 (Vascular Endothelial Growth Factor A) EC 3.4.22.38 (Ctsk protein, mouse) |
| تواريخ الأحداث: | Date Created: 20240306 Date Completed: 20240307 Latest Revision: 20240307 |
| رمز التحديث: | 20240307 |
| PMID: | 38444130 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0371-0874 |
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