دورية أكاديمية
Performance evaluation of different albumin assays for the detection of analbuminemia.
العنوان: | Performance evaluation of different albumin assays for the detection of analbuminemia. |
---|---|
المؤلفون: | Zhang Y; Department of Nutrition Science, Purdue University, West Lafayette, IN, United States of America., Abdollahi A; Department of Nutrition Science, Purdue University, West Lafayette, IN, United States of America., Andolino C; Purdue Proteomics Facility, Purdue University, West Lafayette, IN, United States of America., Tomoo K; Department of Nutrition Science, Purdue University, West Lafayette, IN, United States of America., Foster BM; Department of Nutrition Science, Purdue University, West Lafayette, IN, United States of America., Aryal UK; Purdue Proteomics Facility, Purdue University, West Lafayette, IN, United States of America.; Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, United States of America., Henderson GC; Department of Nutrition Science, Purdue University, West Lafayette, IN, United States of America. |
المصدر: | PloS one [PLoS One] 2024 Mar 06; Vol. 19 (3), pp. e0300130. Date of Electronic Publication: 2024 Mar 06 (Print Publication: 2024). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science |
مواضيع طبية MeSH: | Tandem Mass Spectrometry* , Albumins*, Animals ; Mice ; Chromatography, Liquid ; Amino Acid Sequence ; Biological Assay ; Bromcresol Green ; Bromcresol Purple |
مستخلص: | Analbuminemia is characterized by the near absence of albumin in the plasma. Different methods are available for measuring albumin levels, but they do not necessarily agree with one another. It is a concern that analbuminemic samples could be falsely characterized due to the incorrect estimation of albumin. The objective of the work was to evaluate the performance of different assays in detecting analbuminemia. Albumin knockout (Alb-/-) mouse plasma was used to test the suitability of different albumin assays for their ability to properly characterize extreme albumin deficiency. Bromocresol green (BCG), bromocresol purple (BCP), enzyme-linked immunosorbent assay (ELISA), liquid chromatography-tandem mass spectrometry (LC-MS/MS), and gel electrophoresis were tested. The LC-MS/MS assay exhibited broad coverage of the amino acid sequence of albumin and indicated 8,400-fold lower (P<0.0001) albumin expression in Alb-/- than wildtype (WT), demonstrating its suitability for identifying extreme albumin deficiency. ELISA estimated albumin at 1.5±0.1 g/dL in WT and was below the detection limit in all Alb-/- samples. Gel electrophoresis yielded consistent results with LC-MS/MS and ELISA. The BCG assay overestimated albumin with apparently appreciable albumin concentrations in Alb-/- mice, yet the assay still indicated a significant difference between genotypes (Alb-/-, 1.2±0.05 g/dL, WT, 3.7±0.1 g/dL, P<0.0001). BCP drastically overestimated albumin and could not successfully identify the known analbuminemic phenotype of Alb-/- mice. By using Alb-/- plasma as a reference material and LC-MS/MS as a reference method, ELISA and gel electrophoresis appear appropriate for identifying analbuminemia, while BCG and BCP are not suitable. It is concluded that dye-binding assays should be avoided when extreme hypoalbuminemia or analbuminemia is suspected. Competing Interests: The authors have declared that no competing interests exist. (Copyright: © 2024 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.) |
References: | J Vet Diagn Invest. 2015 Nov;27(6):688-95. (PMID: 26450839) J Aquat Anim Health. 2020 Jun;32(2):77-82. (PMID: 32012365) Ann Clin Biochem. 2016 Jan;53(Pt 1):97-105. (PMID: 25673775) Med Clin (Barc). 2020 Apr 10;154(7):275-278. (PMID: 31928748) Am J Kidney Dis. 1993 Feb;21(2):138-44. (PMID: 8430673) Lipids Health Dis. 2023 Jun 21;22(1):78. (PMID: 37344835) Clin Chem Lab Med. 2023 Jul 04;61(12):2167-2177. (PMID: 37401696) Nutrients. 2023 Apr 25;15(9):. (PMID: 37432201) Nephrol Dial Transplant. 2000 Nov;15(11):1819-22. (PMID: 11071971) Biochem Med (Zagreb). 2014 Feb 15;24(1):151-8. (PMID: 24627724) Biochim Biophys Acta. 2013 Dec;1830(12):5494-502. (PMID: 23612153) World J Clin Cases. 2019 Feb 26;7(4):466-472. (PMID: 30842957) Clin Chim Acta. 2006 Mar;365(1-2):188-93. (PMID: 16183048) Clin Chim Acta. 2010 Nov 11;411(21-22):1711-5. (PMID: 20638375) Eur J Clin Invest. 2013 Jan;43(1):72-8. (PMID: 23176518) Clin Chem. 2017 Mar;63(3):770-779. (PMID: 28073902) MAbs. 2015;7(2):344-51. (PMID: 25654695) Lab Med. 2018 Oct 11;49(4):355-361. (PMID: 29790973) Ren Fail. 2003 Sep;25(5):787-96. (PMID: 14575287) Biochem Med (Zagreb). 2016;26(2):264-71. (PMID: 27346974) Clin Chim Acta. 1986 Feb 28;155(1):83-7. (PMID: 3698308) Kidney Int. 2019 Jul;96(1):248-249. (PMID: 31229037) Clin Chim Acta. 1987 Jul 30;167(1):89-96. (PMID: 3665090) Clin Chem Lab Med. 2012 Dec;50(12):2221-3. (PMID: 23093284) Pathology. 2018 Oct;50(6):679-682. (PMID: 30143345) Ann Lab Med. 2018 Mar;38(2):185-188. (PMID: 29214768) Kidney Int. 2019 Jun;95(6):1514-1517. (PMID: 31053386) Biomolecules. 2023 Feb 22;13(3):. (PMID: 36979342) Clin Chem. 1998 Nov;44(11):2365-7. (PMID: 9799768) Clin Chim Acta. 2014 Jul 1;434:6-10. (PMID: 24709253) Physiol Rep. 2022 Mar;10(5):e15161. (PMID: 35238481) Clin Biochem. 1983 Jun;16(3):178-81. (PMID: 6851081) Clin Chim Acta. 2017 Jun;469:150-160. (PMID: 28410855) Ann Clin Biochem. 1983 Sep;20 (Pt 5):264-70. (PMID: 6651190) |
المشرفين على المادة: | 0 (Albumins) 8YGN0Y942M (Bromcresol Green) 201C22C3EC (Bromcresol Purple) |
تواريخ الأحداث: | Date Created: 20240306 Date Completed: 20240308 Latest Revision: 20240308 |
رمز التحديث: | 20240308 |
مُعرف محوري في PubMed: | PMC10917273 |
DOI: | 10.1371/journal.pone.0300130 |
PMID: | 38446837 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1932-6203 |
---|---|
DOI: | 10.1371/journal.pone.0300130 |