دورية أكاديمية
أعرض في EDS

Performance evaluation of different albumin assays for the detection of analbuminemia.

التفاصيل البيبلوغرافية
العنوان: Performance evaluation of different albumin assays for the detection of analbuminemia.
المؤلفون: Zhang Y; Department of Nutrition Science, Purdue University, West Lafayette, IN, United States of America., Abdollahi A; Department of Nutrition Science, Purdue University, West Lafayette, IN, United States of America., Andolino C; Purdue Proteomics Facility, Purdue University, West Lafayette, IN, United States of America., Tomoo K; Department of Nutrition Science, Purdue University, West Lafayette, IN, United States of America., Foster BM; Department of Nutrition Science, Purdue University, West Lafayette, IN, United States of America., Aryal UK; Purdue Proteomics Facility, Purdue University, West Lafayette, IN, United States of America.; Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, United States of America., Henderson GC; Department of Nutrition Science, Purdue University, West Lafayette, IN, United States of America.
المصدر: PloS one [PLoS One] 2024 Mar 06; Vol. 19 (3), pp. e0300130. Date of Electronic Publication: 2024 Mar 06 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Tandem Mass Spectrometry* , Albumins*, Animals ; Mice ; Chromatography, Liquid ; Amino Acid Sequence ; Biological Assay ; Bromcresol Green ; Bromcresol Purple
مستخلص: Analbuminemia is characterized by the near absence of albumin in the plasma. Different methods are available for measuring albumin levels, but they do not necessarily agree with one another. It is a concern that analbuminemic samples could be falsely characterized due to the incorrect estimation of albumin. The objective of the work was to evaluate the performance of different assays in detecting analbuminemia. Albumin knockout (Alb-/-) mouse plasma was used to test the suitability of different albumin assays for their ability to properly characterize extreme albumin deficiency. Bromocresol green (BCG), bromocresol purple (BCP), enzyme-linked immunosorbent assay (ELISA), liquid chromatography-tandem mass spectrometry (LC-MS/MS), and gel electrophoresis were tested. The LC-MS/MS assay exhibited broad coverage of the amino acid sequence of albumin and indicated 8,400-fold lower (P<0.0001) albumin expression in Alb-/- than wildtype (WT), demonstrating its suitability for identifying extreme albumin deficiency. ELISA estimated albumin at 1.5±0.1 g/dL in WT and was below the detection limit in all Alb-/- samples. Gel electrophoresis yielded consistent results with LC-MS/MS and ELISA. The BCG assay overestimated albumin with apparently appreciable albumin concentrations in Alb-/- mice, yet the assay still indicated a significant difference between genotypes (Alb-/-, 1.2±0.05 g/dL, WT, 3.7±0.1 g/dL, P<0.0001). BCP drastically overestimated albumin and could not successfully identify the known analbuminemic phenotype of Alb-/- mice. By using Alb-/- plasma as a reference material and LC-MS/MS as a reference method, ELISA and gel electrophoresis appear appropriate for identifying analbuminemia, while BCG and BCP are not suitable. It is concluded that dye-binding assays should be avoided when extreme hypoalbuminemia or analbuminemia is suspected.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (Albumins)
8YGN0Y942M (Bromcresol Green)
201C22C3EC (Bromcresol Purple)
تواريخ الأحداث: Date Created: 20240306 Date Completed: 20240308 Latest Revision: 20240308
رمز التحديث: 20240308
مُعرف محوري في PubMed: PMC10917273
DOI: 10.1371/journal.pone.0300130
PMID: 38446837
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0300130