دورية أكاديمية
Sperm traits and seminal plasma proteome of locally adapted hairy rams subjected to intermittent scrotal insulation.
| العنوان: | Sperm traits and seminal plasma proteome of locally adapted hairy rams subjected to intermittent scrotal insulation. |
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| المؤلفون: | Viana Neto AM; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil., Guerreiro DD; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil., Martins JAM; School of Veterinary Medicine, Federal University of Cariri, Juazeiro do Norte, Brazil., Vasconcelos FÁR; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil., Melo RÉBF; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil., Velho ALMCS; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil., Neila-Montero M; Itra-ULE, Animal Reproduction and Obstetrics, Department of Veterinary Medicine, Surgery and Anatomy, University of León, León, Spain., Montes-Garrido R; Itra-ULE, Animal Reproduction and Obstetrics, Department of Veterinary Medicine, Surgery and Anatomy, University of León, León, Spain., Nagano CS; Department of Fisheries Engineering, Federal University of Ceará, Fortaleza, Brazil., Araújo AA; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; School of Veterinary Medicine, Ceará State University, Fortaleza, Brazil., Moura AA; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil. Electronic address: arlindo.moura@gmail.com. |
| المصدر: | Animal reproduction science [Anim Reprod Sci] 2024 Apr; Vol. 263, pp. 107439. Date of Electronic Publication: 2024 Feb 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7807205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2232 (Electronic) Linking ISSN: 03784320 NLM ISO Abbreviation: Anim Reprod Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier Scientific Pub. Co. |
| مواضيع طبية MeSH: | Semen*/physiology , Proteome*/metabolism, Male ; Sheep ; Animals ; Testis/physiology ; Sperm Motility ; Spermatozoa/physiology ; Sheep, Domestic |
| مستخلص: | The present study evaluated the effects of heat stress on reproductive parameters of hairy rams. Six animals were subjected to scrotal insulation during four consecutive nights (6 PM - 6 AM). Day (D) 0 was the first day of insulation. Scrotal circumference increased from 30.5 ± 0.3 cm (at pre-insulation) to 31.8 ± 0.4 cm on D4, decreased 3.9 cm on D28, returning to 30.6 ± 0.6 cm on D57. Sperm concentration decreased from 3.7 ± 0.12 ×10 9 sperm/mL before insulation to 2.6 ± 0.1 ×10 9 on D23, returning to normal on D57. Sperm motility averaged 75 ± 2.9% before insulation, was undetectable on D23, and became normal on D77. Sperm with normal morphology reached 5.9 ± 2.6% on D35 but recovered (86.8 ± 2.1%) on D91. Sperm DNA integrity decreased from 86.5 ± 4.7% before insulation to 11.1 ± 3.7% on D63, returning to pre-insulation values on D120. Sperm BSP immunostaining was reduced after scrotal insulation. Variations in seminal protein abundances coincided with changes in sperm parameters. Seminal plasma superoxide dismutase, carboxypeptidase Q-precursor and NPC intracellular cholesterol transporter 2 decreased on D18, returning to normal after D28. Albumin, inhibitor of carbonic anhydrase precursor, EGF-like repeat and discoid I-like domain-containing protein 3 and polymeric immunoglobulin receptor increased after insulation. In summary, intermittent scrotal insulation drastically altered ram sperm attributes and seminal proteins, especially those associated with oxidative stress. Knowledge of animal´s response to thermal stress is vital in the scenario of climate changes. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Binder of sperm proteins; Heat stress; Seminal plasma; Sperm; Testis |
| المشرفين على المادة: | 0 (Proteome) |
| تواريخ الأحداث: | Date Created: 20240306 Date Completed: 20240401 Latest Revision: 20240401 |
| رمز التحديث: | 20240401 |
| DOI: | 10.1016/j.anireprosci.2024.107439 |
| PMID: | 38447240 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2232 |
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| DOI: | 10.1016/j.anireprosci.2024.107439 |