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Regulatory activity is the default DNA state in eukaryotes.

التفاصيل البيبلوغرافية
العنوان: Regulatory activity is the default DNA state in eukaryotes.
المؤلفون: Luthra I; School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada., Jensen C; School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada., Chen XE; School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada., Salaudeen AL; School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada., Rafi AM; School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada., de Boer CG; School of Biomedical Engineering, University of British Columbia, Vancouver, British Columbia, Canada. carl.deboer@ubc.ca.
المصدر: Nature structural & molecular biology [Nat Struct Mol Biol] 2024 Mar; Vol. 31 (3), pp. 559-567. Date of Electronic Publication: 2024 Mar 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101186374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1545-9985 (Electronic) Linking ISSN: 15459985 NLM ISO Abbreviation: Nat Struct Mol Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York : Nature Pub. Group, c2004-
مواضيع طبية MeSH: Saccharomyces cerevisiae*/genetics , Transcriptome*, Humans ; Genome ; DNA ; Chromatin
مستخلص: Genomes encode for genes and non-coding DNA, both capable of transcriptional activity. However, unlike canonical genes, many transcripts from non-coding DNA have limited evidence of conservation or function. Here, to determine how much biological noise is expected from non-genic sequences, we quantify the regulatory activity of evolutionarily naive DNA using RNA-seq in yeast and computational predictions in humans. In yeast, more than 99% of naive DNA bases were transcribed. Unlike the evolved transcriptome, naive transcripts frequently overlapped with opposite sense transcripts, suggesting selection favored coherent gene structures in the yeast genome. In humans, regulation-associated chromatin activity is predicted to be common in naive dinucleotide-content-matched randomized DNA. Here, naive and evolved DNA have similar co-occurrence and cell-type specificity of chromatin marks, challenging these as indicators of selection. However, in both yeast and humans, extreme high activities were rare in naive DNA, suggesting they result from selection. Overall, basal regulatory activity seems to be the default, which selection can hone to evolve a function or, if detrimental, repress.
(© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.)
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المشرفين على المادة: 9007-49-2 (DNA)
0 (Chromatin)
تواريخ الأحداث: Date Created: 20240306 Date Completed: 20240320 Latest Revision: 20240323
رمز التحديث: 20240323
DOI: 10.1038/s41594-024-01235-4
PMID: 38448573
قاعدة البيانات: MEDLINE
الوصف
تدمد:1545-9985
DOI:10.1038/s41594-024-01235-4