دورية أكاديمية
Universal recording of immune cell interactions in vivo.
| العنوان: | Universal recording of immune cell interactions in vivo. |
|---|---|
| المؤلفون: | Nakandakari-Higa S; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA., Walker S; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA.; Department of Quantitative and Computational Biology, Princeton University, Princeton, NJ, USA., Canesso MCC; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA.; Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA., van der Heide V; Marc and Jennifer Lipschultz Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Tisch Cancer Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Chudnovskiy A; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA., Kim DY; Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA., Jacobsen JT; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA.; Institute for Immunology and Transfusion Medicine, Rikshospitalet, University of Oslo, Oslo, Norway., Parsa R; Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA., Bilanovic J; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA., Parigi SM; Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY, USA., Fiedorczuk K; Laboratory of Membrane Biology and Biophysics, The Rockefeller University, New York, NY, USA., Fuchs E; Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY, USA.; Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA., Bilate AM; Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA., Pasqual G; Laboratory of Synthetic Immunology, Department of Surgery, Oncology and Gastroenterology, University of Padova, Padua, Italy., Mucida D; Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA.; Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA., Kamphorst AO; Marc and Jennifer Lipschultz Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Tisch Cancer Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Pritykin Y; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA. pritykin@princeton.edu.; Department of Computer Science, Princeton University, Princeton, NJ, USA. pritykin@princeton.edu., Victora GD; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA. victora@rockefeller.edu. |
| المصدر: | Nature [Nature] 2024 Mar; Vol. 627 (8003), pp. 399-406. Date of Electronic Publication: 2024 Mar 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Basingstoke : Nature Publishing Group Original Publication: London, Macmillan Journals ltd. |
| مواضيع طبية MeSH: | CD8-Positive T-Lymphocytes*/cytology , CD8-Positive T-Lymphocytes*/immunology , Cell Communication*/immunology , Dendritic Cells*/cytology , Dendritic Cells*/immunology , T-Lymphocytes, Regulatory*/cytology , T-Lymphocytes, Regulatory*/immunology , T Follicular Helper Cells*/cytology , T Follicular Helper Cells*/immunology , B-Lymphocytes*/cytology , B-Lymphocytes*/immunology , Epithelial Cells*/cytology , Epithelial Cells*/immunology, Ligands ; Germinal Center/cytology ; Single-Cell Gene Expression Analysis ; Intestinal Mucosa/cytology ; Intestinal Mucosa/immunology ; Lymphocytic choriomeningitis virus/immunology ; Lymphocytic Choriomeningitis/immunology ; Lymphocytic Choriomeningitis/virology ; Organ Specificity |
| مستخلص: | Immune cells rely on transient physical interactions with other immune and non-immune populations to regulate their function 1 . To study these 'kiss-and-run' interactions directly in vivo, we previously developed LIPSTIC (labelling immune partnerships by SorTagging intercellular contacts) 2 , an approach that uses enzymatic transfer of a labelled substrate between the molecular partners CD40L and CD40 to label interacting cells. Reliance on this pathway limited the use of LIPSTIC to measuring interactions between CD4 + T helper cells and antigen-presenting cells, however. Here we report the development of a universal version of LIPSTIC (uLIPSTIC), which can record physical interactions both among immune cells and between immune and non-immune populations irrespective of the receptors and ligands involved. We show that uLIPSTIC can be used, among other things, to monitor the priming of CD8 + T cells by dendritic cells, reveal the steady-state cellular partners of regulatory T cells and identify germinal centre-resident T follicular helper cells on the basis of their ability to interact cognately with germinal centre B cells. By coupling uLIPSTIC with single-cell transcriptomics, we build a catalogue of the immune populations that physically interact with intestinal epithelial cells at the steady state and profile the evolution of the interactome of lymphocytic choriomeningitis virus-specific CD8 + T cells in multiple organs following systemic infection. Thus, uLIPSTIC provides a broadly useful technology for measuring and understanding cell-cell interactions across multiple biological systems. (© 2024. The Author(s), under exclusive licence to Springer Nature Limited.) |
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| معلومات مُعتمدة: | R01 AI173086 United States AI NIAID NIH HHS; R37 AR027883 United States AR NIAMS NIH HHS; R01 AI153363 United States AI NIAID NIH HHS; R01 AR050452 United States AR NIAMS NIH HHS; DP2 AI171161 United States AI NIAID NIH HHS; T32 HG003284 United States HG NHGRI NIH HHS; R01 AR027883 United States AR NIAMS NIH HHS; United Kingdom WT_ Wellcome Trust; DP1 AI144248 United States AI NIAID NIH HHS |
| المشرفين على المادة: | 0 (Ligands) |
| تواريخ الأحداث: | Date Created: 20240306 Date Completed: 20240315 Latest Revision: 20240605 |
| رمز التحديث: | 20240606 |
| مُعرف محوري في PubMed: | PMC11078586 |
| DOI: | 10.1038/s41586-024-07134-4 |
| PMID: | 38448581 |
| قاعدة البيانات: | MEDLINE |
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| DOI: | 10.1038/s41586-024-07134-4 |