دورية أكاديمية
Rapid and sensitive quantitation of amitraz in orange, tomato, and eggplant samples using immunochromatographic assay.
| العنوان: | Rapid and sensitive quantitation of amitraz in orange, tomato, and eggplant samples using immunochromatographic assay. |
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| المؤلفون: | Lu Q; State Key Laboratory of Food Science and Resources, Jiangnan University, China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China., Liu L; State Key Laboratory of Food Science and Resources, Jiangnan University, China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China., Li J; State Key Laboratory of Food Science and Resources, Jiangnan University, China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China., Song S; State Key Laboratory of Food Science and Resources, Jiangnan University, China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China., Kuang H; State Key Laboratory of Food Science and Resources, Jiangnan University, China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China., Xu C; State Key Laboratory of Food Science and Resources, Jiangnan University, China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China., Guo L; State Key Laboratory of Food Science and Resources, Jiangnan University, China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: gling0329@jiangnan.edu.cn. |
| المصدر: | Food chemistry [Food Chem] 2024 Jul 15; Vol. 446, pp. 138899. Date of Electronic Publication: 2024 Mar 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Barking : Elsevier Applied Science Publishers Original Publication: Barking, Eng., Applied Science Publishers. |
| مواضيع طبية MeSH: | Solanum lycopersicum* , Solanum melongena* , Citrus sinensis* , Insecticides* , Toluidines*, Chromatography, Liquid ; Antibodies, Monoclonal ; Tandem Mass Spectrometry ; Immunoassay/methods ; Limit of Detection ; Chromatography, Affinity/methods ; Enzyme-Linked Immunosorbent Assay |
| مستخلص: | Amitraz (AMT) is a broad-spectrum formamidine insecticide and acaricide. In this study, we produced an anti-AMT monoclonal antibody (mAb) with high performance. The half-maximal inhibitory concentration of the anti-AMT mAb was 4.418 ng/mL, the cross reactivity with other insecticides was negligible, and an affinity constant was 2.06 × 10 9 mmol/L. Additionally, we developed an immunochromatographic assay for the rapid detection of AMT residues in oranges, tomatoes, and eggplants. The cut-off values were 2000 μg/kg in oranges and tomato samples and 1000 μg/kg in eggplant samples and the calculated limits of detection were 14.521 μg/kg, 6.281 μg/kg, and 3.518 μg/kg in oranges, tomatoes, and eggplants, respectively, meeting the detection requirements for AMT in fruits and vegetables. The recovery rates ranged between 95.8 % and 105.2 %, consistent with the recovery rates obtained via LC-MS/MS. Our developed immunochromatographic assay can effectively, accurately, and rapidly determine AMT residues in oranges, tomatoes, and eggplants. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Amitraz; Eggplants; Immunochromatographic assay; Monoclonal antibody; Oranges; Tomatoes |
| المشرفين على المادة: | 33IAH5017S (amitraz) 0 (Antibodies, Monoclonal) 0 (Insecticides) 0 (Toluidines) |
| تواريخ الأحداث: | Date Created: 20240307 Date Completed: 20240403 Latest Revision: 20240403 |
| رمز التحديث: | 20240403 |
| DOI: | 10.1016/j.foodchem.2024.138899 |
| PMID: | 38452506 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-7072 |
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| DOI: | 10.1016/j.foodchem.2024.138899 |