دورية أكاديمية
أعرض في EDS

EvfG is a multi-function protein located in the Type VI secretion system for ExPEC.

التفاصيل البيبلوغرافية
العنوان: EvfG is a multi-function protein located in the Type VI secretion system for ExPEC.
المؤلفون: Lu W; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; Hubei Hongshan Laboratory, Wuhan, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China., Lu H; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China., Huo X; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China., Wang C; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China., Zhang Z; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China., Zong B; School of animal science and nutrition engineering, Wuhan Polytechnic University, Wuhan 430023, China., Wang G; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China., Dong W; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China., Li X; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China., Li Y; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China., Chen H; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; Hubei Hongshan Laboratory, Wuhan, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China., Tan C; National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China; Hubei Hongshan Laboratory, Wuhan, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, China. Electronic address: tanchen@mail.hzau.edu.cn.
المصدر: Microbiological research [Microbiol Res] 2024 Jun; Vol. 283, pp. 127647. Date of Electronic Publication: 2024 Feb 23.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: G. Fischer Country of Publication: Germany NLM ID: 9437794 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-0623 (Electronic) Linking ISSN: 09445013 NLM ISO Abbreviation: Microbiol Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Jena : G. Fischer, c1994-
مواضيع طبية MeSH: Type VI Secretion Systems*/genetics , Type VI Secretion Systems*/metabolism , Extraintestinal Pathogenic Escherichia coli*/metabolism , Escherichia coli Proteins*/genetics , Escherichia coli Proteins*/metabolism, Virulence ; Virulence Factors/genetics ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism
مستخلص: The Type VI secretion system (T6SS) functions as a protein transport nanoweapon in several stages of bacterial life. Even though bacterial competition is the primary function of T6SS, different bacteria exhibit significant variations. Particularly in Extraintestinal pathogenic Escherichia coli (ExPEC), research into T6SS remains relatively limited. This study identified the uncharacterized gene evfG within the T6SS cluster of ExPEC RS218. Through our experiments, we showed that evfG is involved in T6SS expression in ExPEC RS218. We also found evfG can modulate T6SS activity by competitively binding to c-di-GMP, leading to a reduction in the inhibitory effect. Furthermore, we found that evfG can recruit sodA to alleviate oxidative stress. The research shown evfG controls an array of traits, both directly and indirectly, through transcriptome and additional tests. These traits include cell adhesion, invasion, motility, drug resistance, and pathogenicity of microorganisms. Overall, we contend that evfG serves as a multi-functional regulator for the T6SS and several crucial activities. This forms the basis for the advancement of T6SS function research, as well as new opportunities for vaccine and medication development.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
(Copyright © 2024. Published by Elsevier GmbH.)
فهرسة مساهمة: Keywords: C-di-GMP; Drug target; Motility; Oxidative stress; T6SS; evfG
المشرفين على المادة: 0 (Type VI Secretion Systems)
0 (Escherichia coli Proteins)
0 (Virulence Factors)
0 (Bacterial Proteins)
تواريخ الأحداث: Date Created: 20240307 Date Completed: 20240417 Latest Revision: 20240417
رمز التحديث: 20240417
DOI: 10.1016/j.micres.2024.127647
PMID: 38452551
قاعدة البيانات: MEDLINE
الوصف
تدمد:1618-0623
DOI:10.1016/j.micres.2024.127647