دورية أكاديمية
Improvement of survivability and developmental ability in vitrified rat oocytes.
| العنوان: | Improvement of survivability and developmental ability in vitrified rat oocytes. |
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| المؤلفون: | Nakagawa Y; Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University, Iwate, 020-8551, Japan., Kaneko T; Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University, Iwate, 020-8551, Japan; Division of Fundamental and Applied Sciences, Graduate School of Science and Engineering, Iwate University, Iwate, 020-8551, Japan. Electronic address: takekaneko5@gmail.com. |
| المصدر: | Cryobiology [Cryobiology] 2024 Jun; Vol. 115, pp. 104882. Date of Electronic Publication: 2024 Mar 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0006252 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2392 (Electronic) Linking ISSN: 00112240 NLM ISO Abbreviation: Cryobiology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Amsterdam : Elsevier Original Publication: San Diego, CA : Academic Press |
| مواضيع طبية MeSH: | Oocytes*/drug effects , Oocytes*/cytology , Vitrification* , Leupeptins*/pharmacology , Cryopreservation*/methods , Embryonic Development*/drug effects , Sperm Injections, Intracytoplasmic*/methods, Animals ; Rats ; Female ; Cell Survival/drug effects ; Male ; Cryoprotective Agents/pharmacology |
| مستخلص: | Oocyte cryopreservation is useful for human fertility treatment and strain preservation in both experimental and domestic animals. However, the embryonic development of vitrified rat oocytes was lower than that of vitrified embryos. To increase the viability of vitrified oocytes, intracellular ice formation during cooling and warming must be prevented. Rapid warming is important to prevent ice formation. Furthermore, suppressing the spontaneous activation of oocytes is also important because vitrification promotes the spontaneous activation of rat oocytes, and thus compromise developmental competence of the gametes. MG132, a proteasome inhibitor, suppresses the spontaneous activation of rat oocytes. Here, we examined the effects of rapid warming and MG132 treatment on the survival and embryonic development of vitrified rat oocytes. The warming rate was adjusted by changing the vitrification solution volume and warming solution temperature. The survival rate of oocytes vitrified in 10 μL solution and warmed at 50 °C (94%) was significantly higher than that of oocytes vitrified in 100 μL and 10 μL solution and warmed at 37 °C (49% and 81%, respectively). Furthermore, the rate of embryonic development of vitrified oocytes treated with MG132 during vitrification, warming, and intracytoplasmic sperm injection (ICSI) (44%) was significantly higher than that of untreated gametes (10%). Offspring were obtained after transferring embryos derived from MG132-treated vitrified oocytes (14%). Altogether, the survivability of vitrified rat oocytes increased by rapid warming, and MG132 improved embryonic development after ICSI. (Copyright © 2024 Society for Cryobiology. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Intracytoplasmic sperm injection; Oocytes; Rat; Vitrification |
| المشرفين على المادة: | 0 (Leupeptins) RF1P63GW3K (benzyloxycarbonylleucyl-leucyl-leucine aldehyde) 0 (Cryoprotective Agents) |
| تواريخ الأحداث: | Date Created: 20240307 Date Completed: 20240609 Latest Revision: 20240609 |
| رمز التحديث: | 20240610 |
| DOI: | 10.1016/j.cryobiol.2024.104882 |
| PMID: | 38452847 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2392 |
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| DOI: | 10.1016/j.cryobiol.2024.104882 |