دورية أكاديمية
Human amniotic mesenchymal stem cells inhibit immune rejection injury from allogeneic mouse heart transplantation: A preliminary study on the microRNA expression.
| العنوان: | Human amniotic mesenchymal stem cells inhibit immune rejection injury from allogeneic mouse heart transplantation: A preliminary study on the microRNA expression. |
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| المؤلفون: | Wang H; Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Zunyi Medical University, Zunyi 563006, Guizhou, China; The Second Clinical Institute, Zunyi Medical University, Zunyi 563006, Guizhou, China; Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou, China., Mao X; Department of Clinical Medical College, Zunyi Medical University, Zunyi 563006, Guizhou, China; Department of Gastroenterology, Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou, China., Zhong Y; Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Zunyi Medical University, Zunyi 563006, Guizhou, China; The Second Clinical Institute, Zunyi Medical University, Zunyi 563006, Guizhou, China., Zhao X; Department of Clinical Medical College, Zunyi Medical University, Zunyi 563006, Guizhou, China., Li C; Department of Clinical Medical College, Zunyi Medical University, Zunyi 563006, Guizhou, China., Jiang J; Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Zunyi Medical University, Zunyi 563006, Guizhou, China; The Second Clinical Institute, Zunyi Medical University, Zunyi 563006, Guizhou, China., Hong Z; Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Zunyi Medical University, Zunyi 563006, Guizhou, China; The Second Clinical Institute, Zunyi Medical University, Zunyi 563006, Guizhou, China., Wang N; Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou, China; The Clinical Stem Cell Research Institute, Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou, China; Collaborative Innovation Center of Chinese Ministry of Education, Zunyi Medical University, Zunyi 563003, Guizhou, China. Electronic address: wangnuoxin@foxmail.com., Wang F; Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Zunyi Medical University, Zunyi 563006, Guizhou, China; The Second Clinical Institute, Zunyi Medical University, Zunyi 563006, Guizhou, China; Department of Cardiovascular Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China. Electronic address: wang1981@stu.gmc.edu.cn. |
| المصدر: | Transplant immunology [Transpl Immunol] 2024 Jun; Vol. 84, pp. 102022. Date of Electronic Publication: 2024 Mar 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9309923 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5492 (Electronic) Linking ISSN: 09663274 NLM ISO Abbreviation: Transpl Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Dunton Green, Sevenoaks, Kent, UK : Edward Arnold, c1993- |
| مواضيع طبية MeSH: | Heart Transplantation* , MicroRNAs*/genetics , MicroRNAs*/metabolism , Graft Rejection*/immunology , Mesenchymal Stem Cells*/metabolism , Mesenchymal Stem Cells*/immunology , Mesenchymal Stem Cell Transplantation* , Transplantation, Homologous* , Amnion*/cytology, Animals ; Mice ; Humans ; Mice, Inbred BALB C ; Cells, Cultured ; Disease Models, Animal ; Mice, Inbred C57BL ; Graft Survival/immunology ; Female ; Male |
| مستخلص: | Background: Mesenchymal stem cell therapy is a new treatment for immune rejection in heart transplantation. The aim of this paper is to investigate the effect of human amniotic mesenchymal stem cells (hAMSCs) on alleviating immune rejection of allogeneic heart transplantation in mice and its possible underlying mechanism. Methods: We injected hAMSCs into cervical ectopic heart transplantation model mice via tail vein to observe the survival time, the pathological changes of donor myocardium, and the fluorescent distribution of hAMSCs after the transplantation. MicroRNAs (miRs) with significantly differential expression were obtained by RNA sequencing and bioinformatic analysis, and a dual luciferase reporter gene assay together with real-time quantitative PCR (qRT-PCR) was performed to verify the relationship between miRs and their targeting genes. Results: The intervention of hAMSCs prolonged the graft survival time and alleviated the pathological damage of the donor heart. The injected hAMSCs were distributed mainly in the liver, spleen, and kidney, only a very small portion in the donor and recipient hearts. In the allogeneic transplantation models, the expression of miR-34b-5p significantly increased after hAMSC treatment. MiR-34b-5p showed a knockdown effect on gene Fc gamma receptor 2B (FCGR2B). Conclusions: hAMSCs can reduce the immune rejection injury after allogeneic heart transplantation. This effect may be associated with the upregulation of miR-34b-5p expression to knock down its targeting gene FCGR2B. Competing Interests: Declaration of competing interest The authors declare that they have no competing interests. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: FCGR2B gene; Heart transplantation; Human amniotic mesenchymal stem cell; Immune rejection; MicroRNA-34b-5p |
| المشرفين على المادة: | 0 (MicroRNAs) |
| تواريخ الأحداث: | Date Created: 20240307 Date Completed: 20240530 Latest Revision: 20240530 |
| رمز التحديث: | 20240531 |
| DOI: | 10.1016/j.trim.2024.102022 |
| PMID: | 38452986 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1878-5492 |
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| DOI: | 10.1016/j.trim.2024.102022 |