دورية أكاديمية
Tissue Usage Preference and Intrinsically Disordered Region Remodeling of Alternative Splicing Derived Proteoforms in the Heart.
العنوان: | Tissue Usage Preference and Intrinsically Disordered Region Remodeling of Alternative Splicing Derived Proteoforms in the Heart. |
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المؤلفون: | Pandi B, Brenman S, Black A, Ng DCM, Lau E, Lam MPY |
المصدر: | Journal of proteome research [J Proteome Res] 2024 Aug 02; Vol. 23 (8), pp. 3161-3173. Date of Electronic Publication: 2024 Mar 08. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101128775 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-3907 (Electronic) Linking ISSN: 15353893 NLM ISO Abbreviation: J Proteome Res Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c2002- |
مواضيع طبية MeSH: | Alternative Splicing* , Protein Isoforms*/genetics , Protein Isoforms*/metabolism , Intrinsically Disordered Proteins*/genetics , Intrinsically Disordered Proteins*/metabolism , Intrinsically Disordered Proteins*/chemistry, Humans ; Heart Ventricles/metabolism ; Proteome/genetics ; Proteome/metabolism ; Heart Atria/metabolism ; Myocardium/metabolism ; Myocardium/chemistry ; LIM Domain Proteins/genetics ; LIM Domain Proteins/metabolism ; LIM Domain Proteins/chemistry ; Mass Spectrometry ; Tensins/metabolism ; Tensins/genetics ; Organ Specificity ; Protein Binding |
مستخلص: | A computational analysis of mass spectrometry data was performed to uncover alternative splicing derived protein variants across chambers of the human heart. Evidence for 216 non-canonical isoforms was apparent in the atrium and the ventricle, including 52 isoforms not documented on SwissProt and recovered using an RNA sequencing derived database. Among non-canonical isoforms, 29 show signs of regulation based on statistically significant preferences in tissue usage, including a ventricular enriched protein isoform of tensin-1 (TNS1) and an atrium-enriched PDZ and LIM Domain 3 (PDLIM3) isoform 2 (PDLIM3-2/ALP-H). Examined variant regions that differ between alternative and canonical isoforms are highly enriched with intrinsically disordered regions. Moreover, over two-thirds of such regions are predicted to function in protein binding and RNA binding. The analysis here lends further credence to the notion that alternative splicing diversifies the proteome by rewiring intrinsically disordered regions, which are increasingly recognized to play important roles in the generation of biological function from protein sequences. |
التعليقات: | Update of: bioRxiv. 2023 Oct 09:2023.10.08.561375. doi: 10.1101/2023.10.08.561375. (PMID: 37873130) |
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معلومات مُعتمدة: | R01 GM144456 United States GM NIGMS NIH HHS; R01 HL141278 United States HL NHLBI NIH HHS; R21 HL150456 United States HL NHLBI NIH HHS; R35 GM146815 United States GM NIGMS NIH HHS |
فهرسة مساهمة: | Keywords: IDR; RBP; alternative splicing; heart tissue usage; intrinsically disordered regions; protein isoforms; protein/RNA binding; proteoform |
المشرفين على المادة: | 0 (Protein Isoforms) 0 (Intrinsically Disordered Proteins) 0 (Proteome) 0 (LIM Domain Proteins) 0 (Tensins) |
تواريخ الأحداث: | Date Created: 20240308 Date Completed: 20240802 Latest Revision: 20240808 |
رمز التحديث: | 20240808 |
مُعرف محوري في PubMed: | PMC11296937 |
DOI: | 10.1021/acs.jproteome.3c00789 |
PMID: | 38456420 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1535-3907 |
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DOI: | 10.1021/acs.jproteome.3c00789 |