دورية أكاديمية
A pathogenesis-related protein, PRP1, negatively regulates root nodule symbiosis in Lotus japonicus.
| العنوان: | A pathogenesis-related protein, PRP1, negatively regulates root nodule symbiosis in Lotus japonicus. |
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| المؤلفون: | Li H; National Key Lab of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China., Ou Y; National Key Lab of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China., Huang K; National Key Lab of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China., Zhang Z; National Key Lab of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China., Cao Y; National Key Lab of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China., Zhu H; National Key Lab of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China. |
| المصدر: | Journal of experimental botany [J Exp Bot] 2024 Jun 07; Vol. 75 (11), pp. 3542-3556. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9882906 Publication Model: Print Cited Medium: Internet ISSN: 1460-2431 (Electronic) Linking ISSN: 00220957 NLM ISO Abbreviation: J Exp Bot Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford, UK : Oxford University Press, |
| مواضيع طبية MeSH: | Lotus*/genetics , Lotus*/microbiology , Lotus*/physiology , Symbiosis* , Plant Proteins*/genetics , Plant Proteins*/metabolism , Root Nodules, Plant*/microbiology , Root Nodules, Plant*/genetics , Root Nodules, Plant*/metabolism, Rhizobium/physiology ; Gene Expression Regulation, Plant |
| مستخلص: | The legume-rhizobium symbiosis represents a unique model within the realm of plant-microbe interactions. Unlike typical cases of pathogenic invasion, the infection of rhizobia and their residence within symbiotic cells do not elicit a noticeable immune response in plants. Nevertheless, there is still much to uncover regarding the mechanisms through which plant immunity influences rhizobial symbiosis. In this study, we identify an important player in this intricate interplay: Lotus japonicus PRP1, which serves as a positive regulator of plant immunity but also exhibits the capacity to decrease rhizobial colonization and nitrogen fixation within nodules. The PRP1 gene encodes an uncharacterized protein and is named Pathogenesis-Related Protein1, owing to its orthologue in Arabidopsis thaliana, a pathogenesis-related family protein (At1g78780). The PRP1 gene displays high expression levels in nodules compared to other tissues. We observed an increase in rhizobium infection in the L. japonicus prp1 mutants, whereas PRP1-overexpressing plants exhibited a reduction in rhizobium infection compared to control plants. Intriguingly, L. japonicus prp1 mutants produced nodules with a pinker colour compared to wild-type controls, accompanied by elevated levels of leghaemoglobin and an increased proportion of infected cells within the prp1 nodules. The transcription factor Nodule Inception (NIN) can directly bind to the PRP1 promoter, activating PRP1 gene expression. Furthermore, we found that PRP1 is a positive mediator of innate immunity in plants. In summary, our study provides clear evidence of the intricate relationship between plant immunity and symbiosis. PRP1, acting as a positive regulator of plant immunity, simultaneously exerts suppressive effects on rhizobial infection and colonization within nodules. (© The Author(s) 2024. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.) |
| معلومات مُعتمدة: | 2019YFA0904700 National Key R&D Program of China; 32200207 National Natural Science Foundation of China; 2019M662652 China Postdoctoral Science Foundation |
| فهرسة مساهمة: | Keywords: Lotus japonicus; Infection thread; leghaemoglobin; nitrogen fixation; nodule inception; nodule symbiosis; plant immunity; rhizobial accommodation |
| المشرفين على المادة: | 0 (Plant Proteins) |
| تواريخ الأحداث: | Date Created: 20240308 Date Completed: 20240607 Latest Revision: 20240617 |
| رمز التحديث: | 20240617 |
| DOI: | 10.1093/jxb/erae103 |
| PMID: | 38457346 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1460-2431 |
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| DOI: | 10.1093/jxb/erae103 |