دورية أكاديمية
Pullulanase pretreatment of highly concentrated maltodextrin solution improves maltose yield during β-amylase-catalyzed saccharification.
| العنوان: | Pullulanase pretreatment of highly concentrated maltodextrin solution improves maltose yield during β-amylase-catalyzed saccharification. |
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| المؤلفون: | Li Z; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Kong H; Key Laboratory of Synergetic and Biological Colloids, Ministry of Education, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Li Z; Key Laboratory of Synergetic and Biological Colloids, Ministry of Education, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi 214122, China., Gu Z; Key Laboratory of Synergetic and Biological Colloids, Ministry of Education, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi 214122, China. Electronic address: zhengbiaogu@jiangnan.edu.cn., Yang Q; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Ban X; Key Laboratory of Synergetic and Biological Colloids, Ministry of Education, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi 214122, China., Hong Y; Key Laboratory of Synergetic and Biological Colloids, Ministry of Education, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi 214122, China., Cheng L; Key Laboratory of Synergetic and Biological Colloids, Ministry of Education, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi 214122, China., Li C; Key Laboratory of Synergetic and Biological Colloids, Ministry of Education, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi 214122, China. Electronic address: licaiming2009@126.com. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2024 Apr; Vol. 264 (Pt 2), pp. 130701. Date of Electronic Publication: 2024 Mar 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | beta-Amylase* , Polysaccharides*, Maltose ; Glycoside Hydrolases ; Starch/chemistry ; Catalysis |
| مستخلص: | Increasing the substrate concentration can effectively reduce energy consumption and result in more economic benefits in the industrial production of maltose, but this process remarkably increases the viscosity, which has a negative effect on saccharification. To improve saccharification efficiency, pullulanase is usually employed. In the conventional process of maltose production, pullulanase is added at the same time with β-amylase or later, but this process seems inefficient when the substrate concentration is high. Herein, a novel method was introduced to enhance the maltose yield under high substrate concentration. The results indicated that the pullulanase pretreatment of highly concentrated maltodextrin solution for 2 h greatly affects the final conversion rate of β-amylase-catalyzed saccharification. The maltose yield reached 80.95 %, which is 11.8 % above the control value. Further examination confirmed that pullulanase pretreatment decreased the number of branch points of maltodextrin and resulted in a high content of oligosaccharides. These linear chains were suitable for β-amylase-catalyzed saccharification to produce maltose. This research offers a new effective and green strategy for starch sugar production. Competing Interests: Declaration of competing interest The authors declare no competing interests. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: High substrate concentration; Maltose production; Pullulanase pretreatment; Saccharification process |
| المشرفين على المادة: | EC 3.2.1.41 (pullulanase) 7CVR7L4A2D (maltodextrin) EC 3.2.1.2 (beta-Amylase) 69-79-4 (Maltose) EC 3.2.1.- (Glycoside Hydrolases) 9005-25-8 (Starch) 0 (Polysaccharides) |
| تواريخ الأحداث: | Date Created: 20240308 Date Completed: 20240410 Latest Revision: 20240410 |
| رمز التحديث: | 20240410 |
| DOI: | 10.1016/j.ijbiomac.2024.130701 |
| PMID: | 38458283 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2024.130701 |