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Analysis of the effects of β-mannanase on immune function and intestinal flora in broilers fed the low energy diet based on 16S rRNA sequencing and metagenomic sequencing.

التفاصيل البيبلوغرافية
العنوان: Analysis of the effects of β-mannanase on immune function and intestinal flora in broilers fed the low energy diet based on 16S rRNA sequencing and metagenomic sequencing.
المؤلفون: Zhang X; State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China., Chen Y; State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China., Lv Z; State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China., Zhou L; Beijing Strowin Biotechnology Co. Ltd., Beijing, 100094, China., Guo Y; State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. Electronic address: guoyum@cau.edu.cn.
المصدر: Poultry science [Poult Sci] 2024 May; Vol. 103 (5), pp. 103581. Date of Electronic Publication: 2024 Feb 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 0401150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3171 (Electronic) Linking ISSN: 00325791 NLM ISO Abbreviation: Poult Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: 2020- : [Cambridge, UK] : Elsevier
Original Publication: Champaign Il : Poultry Science Association
مواضيع طبية MeSH: Chickens*/immunology , Chickens*/microbiology , Animal Feed*/analysis , Diet*/veterinary , Gastrointestinal Microbiome*/drug effects , beta-Mannosidase*/metabolism , beta-Mannosidase*/genetics , RNA, Ribosomal, 16S*/analysis , Random Allocation*, Animals ; Dietary Supplements/analysis ; Male ; Animal Nutritional Physiological Phenomena/drug effects ; Metagenomics
مستخلص: As an enzyme, β-mannanase (BM) can be widely used as feed additive to improve the growth performance of animals. This experiment aimed to determine the effect of the addition of BM to low-energy diet on the immune function and intestinal microflora of broiler chickens. In this study, 384 one-day-old Arbor Acres broilers were randomly divided into 3 groups (8 replicates per group): positive control (PC, received a corn-soybean meal basal diet), negative control (NC, received a low-energy diet with Metabolizable Energy (ME) reduced by 50 kcal/kg) and NC + BM group (NC birds + 100 mg/kg BM). All birds were raised for 42 d. The results showed that BM mitigated the damage of immune function in peripheral blood of broilers caused by the decrease of dietary energy level by increasing the Concanavalin A (Con A) index of stimulation (SI) and macrophages phagocytic activity in the peripheral blood of broilers at 42 d (P < 0.05). The analysis of cecum flora showed that the low-energy diet significantly reduced the observed_species index (P < 0.01), Chao1 index and ACE index (P < 0.05), which reduced the abundance and evenness of species in the cecum of broilers at 21 d. It also significantly reduced the relative abundance of Candidatus_Arthromitus and significantly increased the relative abundance of Pseudomonas in the cecum of broilers at 21 d, while also significantly increasing the relative abundance of Monoglobus at 42 d. BM significantly increased the relative abundance of Lachnospiraceae_UCG-001 and Lachnospiraceae_bacterium_615 in the cecum of broilers at 21 d. In addition, BM inhibited microbial Fatty acid degradation by decreasing the activity of glutaryl-CoA dehydrogenase. Collectively, BM could improve intestinal health by enhancing the immune function of broilers, promoting the proliferation of beneficial bacteria and reducing the number of harmful bacteria, regulating intestinal flora, thereby alleviating the adverse effects of lower dietary energy levels.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
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فهرسة مساهمة: Keywords: broiler; immunity; low-energy diet; mannanase; metagenome
المشرفين على المادة: EC 3.2.1.25 (beta-Mannosidase)
0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20240309 Date Completed: 20240430 Latest Revision: 20240505
رمز التحديث: 20240505
مُعرف محوري في PubMed: PMC11067779
DOI: 10.1016/j.psj.2024.103581
PMID: 38460218
قاعدة البيانات: MEDLINE
الوصف
تدمد:1525-3171
DOI:10.1016/j.psj.2024.103581