دورية أكاديمية
أعرض في EDS

PqsA mutation-mediated enhancement of phage-mediated combat against Pseudomonas aeruginosa .

التفاصيل البيبلوغرافية
العنوان: PqsA mutation-mediated enhancement of phage-mediated combat against Pseudomonas aeruginosa .
المؤلفون: Su Q; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao, China., Lu D; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao, China., Kong J; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao, China., Lin H; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao, China., Xuan G; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao, China., Wang J; State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao, China.
المصدر: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 Feb 26; Vol. 14, pp. 1296777. Date of Electronic Publication: 2024 Feb 26 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media SA Country of Publication: Switzerland NLM ID: 101585359 Publication Model: eCollection Cited Medium: Internet ISSN: 2235-2988 (Electronic) Linking ISSN: 22352988 NLM ISO Abbreviation: Front Cell Infect Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media SA
مواضيع طبية MeSH: Bacteriophages* , Pseudomonas Phages*/genetics , Phage Therapy* , Pseudomonas Infections*/therapy, Humans ; Pseudomonas aeruginosa/genetics ; Mutation
مستخلص: Phage therapy is a potential approach in the biocontrol of foodborne pathogens. However, the emergence of phage resistance and the narrow host range of most phage isolates continue to limit the antimicrobial efficacy of phages. Here, we investigated the potential of the pqsA gene, encoding the anthranilate-CoA ligase enzyme, as an adjuvant for phage therapy. The knockout of the pqsA gene significantly enhanced the bactericidal effect of phages vB_Pae_QDWS and vB_Pae_S1 against Pseudomonas aeruginosa . Under phage infection pressure, the growth of the PaΔ pqsA was significantly inhibited within 8 h compared to the wild-type PAO1. Furthermore, we found that altering phage adsorption is not how PaΔ pqsA responds to phage infection. Although pqsA represents a promising target for enhancing phage killing, it may not be applicable to all phages, such as types vB_Pae_W3 and vB_Pae_TR. Our findings provide new material reserves for the future design of novel phage-based therapeutic strategies.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Su, Lu, Kong, Lin, Xuan and Wang.)
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فهرسة مساهمة: Keywords: P. aeruginosa; enhancement of phage therapy; phage; pqsA; resistance
تواريخ الأحداث: Date Created: 20240312 Date Completed: 20240313 Latest Revision: 20240313
رمز التحديث: 20240313
مُعرف محوري في PubMed: PMC10925624
DOI: 10.3389/fcimb.2024.1296777
PMID: 38469347
قاعدة البيانات: MEDLINE
الوصف
تدمد:2235-2988
DOI:10.3389/fcimb.2024.1296777