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A Wnt10a-Notch signaling axis controls Hertwig's epithelial root sheath cell behaviors during root furcation patterning.

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العنوان: A Wnt10a-Notch signaling axis controls Hertwig's epithelial root sheath cell behaviors during root furcation patterning.
المؤلفون: Sun K; Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China., Yu M; Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China., Wang J; Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China., Zhao H; Chinese Institute for Brain Research, Beijing, China., Liu H; Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China., Feng H; Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China., Liu Y; Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China. pkussliuyang@bjmu.edu.cn., Han D; Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China. donghan@bjmu.edu.cn.
المصدر: International journal of oral science [Int J Oral Sci] 2024 Mar 13; Vol. 16 (1), pp. 25. Date of Electronic Publication: 2024 Mar 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: India NLM ID: 101504351 Publication Model: Electronic Cited Medium: Internet ISSN: 2049-3169 (Electronic) Linking ISSN: 16742818 NLM ISO Abbreviation: Int J Oral Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: Jan. 2012- : Bangalore, India : Nature Publishing Group
Original Publication: Chengdu, Sichuan : West China College of Stomatology, Sichuan University Press
مواضيع طبية MeSH: Tooth Root*/metabolism , Tooth*, Humans ; Female ; Mice ; Animals ; Odontogenesis/genetics ; Signal Transduction ; Dental Enamel ; Epithelial Cells ; Nerve Tissue Proteins/metabolism ; Wnt Proteins/metabolism
مستخلص: Human with bi-allelic WNT10A mutations and epithelial Wnt10a knockout mice present enlarged pulp chamber and apical displacement of the root furcation of multi-rooted teeth, known as taurodontism; thus, indicating the critical role of Wnt10a in tooth root morphogenesis. However, the endogenous mechanism by which epithelial Wnt10a regulates Hertwig's epithelial root sheath (HERS) cellular behaviors and contributes to root furcation patterning remains unclear. In this study, we found that HERS in the presumptive root furcating region failed to elongate at an appropriate horizontal level in K14-Cre;Wnt10a fl/fl mice from post-natal day 0.5 (PN0.5) to PN4.5. EdU assays and immunofluorescent staining of cyclin D1 revealed significantly decreased proliferation activity of inner enamel epithelial (IEE) cells of HERS in K14-Cre;Wnt10a fl/fl mice at PN2.5 and PN3.5. Immunofluorescent staining of E-Cadherin and acetyl-α-Tubulin demonstrated that the IEE cells of HERS tended to divide perpendicularly to the horizontal plane, which impaired the horizontal extension of HERS in the presumptive root furcating region of K14-Cre;Wnt10a fl/fl mice. RNA-seq and immunofluorescence showed that the expressions of Jag1 and Notch2 were downregulated in IEE cells of HERS in K14-Cre;Wnt10a fl/fl mice. Furthermore, after activation of Notch signaling in K14-Cre;Wnt10a fl/fl molars by Notch2 adenovirus and kidney capsule grafts, the root furcation defect was partially rescued. Taken together, our study demonstrates that an epithelial Wnt10a-Notch signaling axis is crucial for modulating HERS cell proper proliferation and horizontal-oriented division during tooth root furcation morphogenesis.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 82100976 National Natural Science Foundation of China (National Science Foundation of China); 82071076 National Natural Science Foundation of China (National Science Foundation of China); 82100976 National Natural Science Foundation of China (National Science Foundation of China); 81600846 National Natural Science Foundation of China (National Science Foundation of China)
المشرفين على المادة: 0 (Wnt10a protein, mouse)
0 (Nerve Tissue Proteins)
0 (Wnt Proteins)
تواريخ الأحداث: Date Created: 20240314 Date Completed: 20240315 Latest Revision: 20240317
رمز التحديث: 20240317
مُعرف محوري في PubMed: PMC10937922
DOI: 10.1038/s41368-024-00288-x
PMID: 38480698
قاعدة البيانات: MEDLINE
الوصف
تدمد:2049-3169
DOI:10.1038/s41368-024-00288-x