دورية أكاديمية
أعرض في EDS

Identification of New Markers of Angiogenic Sprouting Using Transcriptomics: New Role for RND3.

التفاصيل البيبلوغرافية
العنوان: Identification of New Markers of Angiogenic Sprouting Using Transcriptomics: New Role for RND3.
المؤلفون: Abbey CA; Texas A&M Health, Department of Medical Physiology (C.A.A., S.R., S.C., K.J.B.), Texas A&M School of Medicine, Bryan.; Department of Molecular and Cellular Medicine (C.A.A., C.L.D., A.C., T.M.S., G.B.W., K.J.B.), Texas A&M School of Medicine, Bryan., Duran CL; Department of Molecular and Cellular Medicine (C.A.A., C.L.D., A.C., T.M.S., G.B.W., K.J.B.), Texas A&M School of Medicine, Bryan.; Now with Department of Pathology, Albert Einstein College of Medicine, Bronx, NY (C.L.D.)., Chen Z; Center for Genomic and Precision Medicine, Institute of Biosciences and Technology, Houston, TX (Z.C., Y.C., J.C.)., Chen Y; Center for Genomic and Precision Medicine, Institute of Biosciences and Technology, Houston, TX (Z.C., Y.C., J.C.)., Roy S; Texas A&M Health, Department of Medical Physiology (C.A.A., S.R., S.C., K.J.B.), Texas A&M School of Medicine, Bryan., Coffell A; Department of Molecular and Cellular Medicine (C.A.A., C.L.D., A.C., T.M.S., G.B.W., K.J.B.), Texas A&M School of Medicine, Bryan., Sveeggen TM; Department of Molecular and Cellular Medicine (C.A.A., C.L.D., A.C., T.M.S., G.B.W., K.J.B.), Texas A&M School of Medicine, Bryan.; Now with Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha (T.M.S.)., Chakraborty S; Texas A&M Health, Department of Medical Physiology (C.A.A., S.R., S.C., K.J.B.), Texas A&M School of Medicine, Bryan., Wells GB; Department of Molecular and Cellular Medicine (C.A.A., C.L.D., A.C., T.M.S., G.B.W., K.J.B.), Texas A&M School of Medicine, Bryan.; Department of Cell Biology and Genetics (G.B.W.), Texas A&M School of Medicine, Bryan., Chang J; Center for Genomic and Precision Medicine, Institute of Biosciences and Technology, Houston, TX (Z.C., Y.C., J.C.)., Bayless KJ; Texas A&M Health, Department of Medical Physiology (C.A.A., S.R., S.C., K.J.B.), Texas A&M School of Medicine, Bryan.; Department of Molecular and Cellular Medicine (C.A.A., C.L.D., A.C., T.M.S., G.B.W., K.J.B.), Texas A&M School of Medicine, Bryan.
المصدر: Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2024 May; Vol. 44 (5), pp. e145-e167. Date of Electronic Publication: 2024 Mar 14.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 9505803 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1524-4636 (Electronic) Linking ISSN: 10795642 NLM ISO Abbreviation: Arterioscler Thromb Vasc Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: 1998- : Baltimore, Md. : Lippincott Williams & Wilkins
Original Publication: Dallas, TX : American Heart Association, c1995-
مواضيع طبية MeSH: Gene Expression Profiling*/methods , Human Umbilical Vein Endothelial Cells*/metabolism , Neovascularization, Physiologic*/genetics , Proto-Oncogene Proteins c-fos*/genetics , Proto-Oncogene Proteins c-fos*/metabolism , rho GTP-Binding Proteins*/metabolism , rho GTP-Binding Proteins*/genetics , Transcriptome*, Animals ; Humans ; Mice ; Cells, Cultured ; Cyclooxygenase 2/metabolism ; Cyclooxygenase 2/genetics ; Endothelial Cells/metabolism ; Phenotype ; Signal Transduction ; Single-Cell Analysis ; Snail Family Transcription Factors/metabolism ; Snail Family Transcription Factors/genetics
مستخلص: Background: New blood vessel formation requires endothelial cells to transition from a quiescent to an invasive phenotype. Transcriptional changes are vital for this switch, but a comprehensive genome-wide approach focused exclusively on endothelial cell sprout initiation has not been reported.
Methods: Using a model of human endothelial cell sprout initiation, we developed a protocol to physically separate cells that initiate the process of new blood vessel formation (invading cells) from noninvading cells. We used this model to perform multiple transcriptomics analyses from independent donors to monitor endothelial gene expression changes.
Results: Single-cell population analyses, single-cell cluster analyses, and bulk RNA sequencing revealed common transcriptomic changes associated with invading cells. We also found that collagenase digestion used to isolate single cells upregulated the Fos proto-oncogene transcription factor. Exclusion of Fos proto-oncogene expressing cells revealed a gene signature consistent with activation of signal transduction, morphogenesis, and immune responses. Many of the genes were previously shown to regulate angiogenesis and included multiple tip cell markers. Upregulation of SNAI1 (snail family transcriptional repressor 1), PTGS2 (prostaglandin synthase 2), and JUNB (JunB proto-oncogene) protein expression was confirmed in invading cells, and silencing JunB and SNAI1 significantly reduced invasion responses. Separate studies investigated rounding 3, also known as RhoE, which has not yet been implicated in angiogenesis. Silencing rounding 3 reduced endothelial invasion distance as well as filopodia length, fitting with a pathfinding role for rounding 3 via regulation of filopodial extensions. Analysis of in vivo retinal angiogenesis in Rnd3 heterozygous mice confirmed a decrease in filopodial length compared with wild-type littermates.
Conclusions: Validation of multiple genes, including rounding 3, revealed a functional role for this gene signature early in the angiogenic process. This study expands the list of genes associated with the acquisition of a tip cell phenotype during endothelial cell sprout initiation.
Competing Interests: Disclosures None.
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معلومات مُعتمدة: R01 HL095786 United States HL NHLBI NIH HHS; R01 HL141215 United States HL NHLBI NIH HHS; R01 HL148133 United States HL NHLBI NIH HHS; R01 HL150124 United States HL NHLBI NIH HHS
فهرسة مساهمة: Keywords: angiogenesis; cell differentiation; collagenase; immune evasion; morphogenesis; three-dimensional cell culture; vascular endothelial cell
المشرفين على المادة: EC 1.14.99.1 (Cyclooxygenase 2)
0 (FOS protein, human)
0 (Proto-Oncogene Proteins c-fos)
EC 3.6.5.2 (rho GTP-Binding Proteins)
0 (SNAI1 protein, human)
0 (Snail Family Transcription Factors)
EC 3.6.5.2 (RND3 protein, human)
EC 3.6.1.- (Rnd3 protein, mouse)
تواريخ الأحداث: Date Created: 20240314 Date Completed: 20240424 Latest Revision: 20240607
رمز التحديث: 20240607
مُعرف محوري في PubMed: PMC11043006
DOI: 10.1161/ATVBAHA.123.320599
PMID: 38482696
قاعدة البيانات: MEDLINE
الوصف
تدمد:1524-4636
DOI:10.1161/ATVBAHA.123.320599