دورية أكاديمية
Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
العنوان: | Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution. |
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المؤلفون: | Tenggara MK; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Oh SH; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Yang C; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Nariya HK; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Metz AM; Emory National Primate Research Center, Atlanta, GA 30329, USA., Upadhyay AA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Gudipati DR; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Guo L; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., McGhee EG; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Gill K; Emory National Primate Research Center, Atlanta, GA 30329, USA., Viox EG; Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA., Mason RD; Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA., Doria-Rose NA; Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA., Foulds KE; Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA., Mascola JR; Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA., Du Y; Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA 30322, USA; Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, GA 30322, USA., Fu H; Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA 30322, USA; Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, GA 30322, USA; Department of Hematology and Medical Oncology and Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA., Altman JD; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA., Yan Q; Department of Obstetrics and Gynecology, Columbia University Irving Medical Center, New York, NY 10032, USA., Sheng Z; Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., Bosinger SE; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA; Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA., Kong R; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA; Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA. Electronic address: rui.kong@emory.edu. |
المصدر: | Cell reports [Cell Rep] 2024 Mar 26; Vol. 43 (3), pp. 113948. Date of Electronic Publication: 2024 Mar 13. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, c 2012- |
مواضيع طبية MeSH: | HIV Seropositivity* , HIV-1* , HIV Infections*, Animals ; Antibodies, Neutralizing ; HIV Antibodies ; Immunoglobulin G ; Memory B Cells |
مستخلص: | Identifying individual functional B cell receptors (BCRs) is common, but two-dimensional analysis of B cell frequency versus BCR potency would delineate both quantity and quality of antigen-specific memory B cells. We efficiently determine quantitative BCR neutralizing activities using a single-cell-derived antibody supernatant analysis (SCAN) workflow and develop a frequency-potency algorithm to estimate B cell frequencies at various neutralizing activity or binding affinity cutoffs. In an HIV-1 fusion peptide (FP) immunization study, frequency-potency curves elucidate the quantity and quality of FP-specific immunoglobulin G (IgG)+ memory B cells for different animals, time points, and antibody lineages at single-cell resolution. The BCR neutralizing activities are mainly determined by their affinities to soluble envelope trimer. Frequency analysis definitively demonstrates dominant neutralizing antibody lineages. These findings establish SCAN and frequency-potency analyses as promising approaches for general B cell analysis and monoclonal antibody (mAb) discovery. They also provide specific rationales for HIV-1 FP-directed vaccine optimization. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
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معلومات مُعتمدة: | P30 AI050409 United States AI NIAID NIH HHS; U24 AI120134 United States AI NIAID NIH HHS; P51 OD011132 United States OD NIH HHS; R61 AI176583 United States AI NIAID NIH HHS; S10 OD026799 United States OD NIH HHS; R01 AI162267 United States AI NIAID NIH HHS |
فهرسة مساهمة: | Keywords: B cell receptor; BCR; CP: Immunology; HIV-1; SCAN; antibody; frequency; fusion peptide; potency; single-cell-derived antibody supernatant analysis; vaccine |
المشرفين على المادة: | 0 (Antibodies, Neutralizing) 0 (HIV Antibodies) 0 (Immunoglobulin G) |
تواريخ الأحداث: | Date Created: 20240314 Date Completed: 20240401 Latest Revision: 20240425 |
رمز التحديث: | 20240425 |
مُعرف محوري في PubMed: | PMC11003769 |
DOI: | 10.1016/j.celrep.2024.113948 |
PMID: | 38483908 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2211-1247 |
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DOI: | 10.1016/j.celrep.2024.113948 |