دورية أكاديمية
أعرض في EDS

Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.

التفاصيل البيبلوغرافية
العنوان: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
المؤلفون: Tenggara MK; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Oh SH; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Yang C; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Nariya HK; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Metz AM; Emory National Primate Research Center, Atlanta, GA 30329, USA., Upadhyay AA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Gudipati DR; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Guo L; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., McGhee EG; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA., Gill K; Emory National Primate Research Center, Atlanta, GA 30329, USA., Viox EG; Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA., Mason RD; Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA., Doria-Rose NA; Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA., Foulds KE; Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA., Mascola JR; Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA., Du Y; Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA 30322, USA; Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, GA 30322, USA., Fu H; Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, GA 30322, USA; Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, GA 30322, USA; Department of Hematology and Medical Oncology and Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA., Altman JD; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA., Yan Q; Department of Obstetrics and Gynecology, Columbia University Irving Medical Center, New York, NY 10032, USA., Sheng Z; Aaron Diamond AIDS Research Center, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA., Bosinger SE; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA; Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA., Kong R; Emory Vaccine Center, Atlanta, GA 30329, USA; Emory National Primate Research Center, Atlanta, GA 30329, USA; Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA. Electronic address: rui.kong@emory.edu.
المصدر: Cell reports [Cell Rep] 2024 Mar 26; Vol. 43 (3), pp. 113948. Date of Electronic Publication: 2024 Mar 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101573691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-1247 (Electronic) NLM ISO Abbreviation: Cell Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, c 2012-
مواضيع طبية MeSH: HIV Seropositivity* , HIV-1* , HIV Infections*, Animals ; Antibodies, Neutralizing ; HIV Antibodies ; Immunoglobulin G ; Memory B Cells
مستخلص: Identifying individual functional B cell receptors (BCRs) is common, but two-dimensional analysis of B cell frequency versus BCR potency would delineate both quantity and quality of antigen-specific memory B cells. We efficiently determine quantitative BCR neutralizing activities using a single-cell-derived antibody supernatant analysis (SCAN) workflow and develop a frequency-potency algorithm to estimate B cell frequencies at various neutralizing activity or binding affinity cutoffs. In an HIV-1 fusion peptide (FP) immunization study, frequency-potency curves elucidate the quantity and quality of FP-specific immunoglobulin G (IgG)+ memory B cells for different animals, time points, and antibody lineages at single-cell resolution. The BCR neutralizing activities are mainly determined by their affinities to soluble envelope trimer. Frequency analysis definitively demonstrates dominant neutralizing antibody lineages. These findings establish SCAN and frequency-potency analyses as promising approaches for general B cell analysis and monoclonal antibody (mAb) discovery. They also provide specific rationales for HIV-1 FP-directed vaccine optimization.
Competing Interests: Declaration of interests The authors declare no competing interests.
(Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: P30 AI050409 United States AI NIAID NIH HHS; U24 AI120134 United States AI NIAID NIH HHS; P51 OD011132 United States OD NIH HHS; R61 AI176583 United States AI NIAID NIH HHS; S10 OD026799 United States OD NIH HHS; R01 AI162267 United States AI NIAID NIH HHS
فهرسة مساهمة: Keywords: B cell receptor; BCR; CP: Immunology; HIV-1; SCAN; antibody; frequency; fusion peptide; potency; single-cell-derived antibody supernatant analysis; vaccine
المشرفين على المادة: 0 (Antibodies, Neutralizing)
0 (HIV Antibodies)
0 (Immunoglobulin G)
تواريخ الأحداث: Date Created: 20240314 Date Completed: 20240401 Latest Revision: 20240425
رمز التحديث: 20240425
مُعرف محوري في PubMed: PMC11003769
DOI: 10.1016/j.celrep.2024.113948
PMID: 38483908
قاعدة البيانات: MEDLINE
الوصف
تدمد:2211-1247
DOI:10.1016/j.celrep.2024.113948