دورية أكاديمية
Spironolactone metabolite causes falsely increased progesterone in the Abbott Architect immunoassay.
| العنوان: | Spironolactone metabolite causes falsely increased progesterone in the Abbott Architect immunoassay. |
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| المؤلفون: | Sarpong KAN; West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), Department of Biochemistry Cell and Molecular Biology, University of Ghana, Accra, Ghana. Electronic address: kansarpong@ug.edu.gh., Hee Kim S; Division of Endocrinology and Metabolism, Department of Medicine, University of Virginia School of Medicine, Charlottesville, VA 22903, USA; Center for Research in Reproduction, University of Virginia School of Medicine, Charlottesville, VA 22903, USA., McCartney CR; Division of Endocrinology and Metabolism, Department of Medicine, University of Virginia School of Medicine, Charlottesville, VA 22903, USA; Center for Research in Reproduction, University of Virginia School of Medicine, Charlottesville, VA 22903, USA., Wiencek JR; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Bazydlo LAL; Department of Pathology, University of Virginia School of Medicine, Charlottesville, VA 22903, USA. |
| المصدر: | Clinical biochemistry [Clin Biochem] 2024 Apr; Vol. 126, pp. 110747. Date of Electronic Publication: 2024 Mar 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 0133660 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2933 (Electronic) Linking ISSN: 00099120 NLM ISO Abbreviation: Clin Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown, NY : Elsevier Science Original Publication: [Toronto] Canadian Society of Clinical Chemists. |
| مواضيع طبية MeSH: | Spironolactone*/metabolism , Canrenone*/metabolism, Humans ; Progesterone ; Digoxin ; Immunoassay/methods |
| مستخلص: | Background: Immunoassays are important for routine clinical testing and medical diagnosis. However, they are limited by cross-reactivity especially at low analyte concentrations. There is a critical need to investigate compounds that can interfere with immunoassays. Herein, we describe the identification of canrenone, a spironolactone metabolite that falsely increases progesterone concentrations on the Abbott Architect i2000 Immunoassay. Methods: Serum samples and assay diluents were spiked with spironolactone or canrenone and progesterone concentrations were measured on the Architect i2000 and Immulite XPi immunoassay platforms. Blood samples from patients taking spironolactone were analyzed with liquid chromatography-tandem mass spectrometry to evaluate the intrinsic response of progesterone concentrations to the presence of canrenone. Results: We measured approximately 10-fold higher progesterone concentrations on the Abbott Architect i2000 compared to reference immunoassay analyzers (Siemens Immulite XPi and Roche Cobas e601/602), suggesting an analytical error which is unique to the Architect i2000 antibody and/or assay conditions. By measuring serum progesterone after addition of spironolactone or canrenone to serum samples, we found that canrenone falsely increased progesterone on the Architect i2000 immunoassay. However, this interference was more pronounced at low serum progesterone concentrations. Moreover, a strong positive correlation was seen between canrenone and measured serum progesterone concentrations. Conclusions: Our investigations are important for individuals who require progesterone measurements using the Architect i2000 immunoassay, especially because it is unlikely for clinicians to order canrenone measurements alongside progesterone measurements for individuals taking spironolactone. Further research is needed to determine whether canrenone can influence progesterone measurements on other immunoassay systems. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Canrenone; Immunoassay; Interference; Progesterone; Spironolactone |
| المشرفين على المادة: | 27O7W4T232 (Spironolactone) 78O20X9J0U (Canrenone) 4G7DS2Q64Y (Progesterone) 73K4184T59 (Digoxin) |
| تواريخ الأحداث: | Date Created: 20240314 Date Completed: 20240401 Latest Revision: 20240401 |
| رمز التحديث: | 20240401 |
| DOI: | 10.1016/j.clinbiochem.2024.110747 |
| PMID: | 38484829 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2933 |
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| DOI: | 10.1016/j.clinbiochem.2024.110747 |