دورية أكاديمية
The genomic and enzymatic basis for iridoid biosynthesis in cat thyme (Teucrium marum).
| العنوان: | The genomic and enzymatic basis for iridoid biosynthesis in cat thyme (Teucrium marum). |
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| المؤلفون: | Smit SJ; Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, UK., Ayten S; Institute of Plant Breeding, Genetics, & Genomics, University of Georgia, Athens, Georgia, 30602, USA., Radzikowska BA; Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, UK.; Department of Chemistry, University of York, York, YO10 5DD, UK., Hamilton JP; Center for Applied Genetic Technologies, University of Georgia, Athens, Georgia, 30602, USA.; Department of Crop & Soil Sciences, University of Georgia, Athens, Georgia, 30602, USA., Langer S; Bioscience Technology Facility, Department of Biology, University of York, York, YO10 5DD, UK., Unsworth WP; Department of Chemistry, University of York, York, YO10 5DD, UK., Larson TR; Bioscience Technology Facility, Department of Biology, University of York, York, YO10 5DD, UK., Buell CR; Institute of Plant Breeding, Genetics, & Genomics, University of Georgia, Athens, Georgia, 30602, USA.; Center for Applied Genetic Technologies, University of Georgia, Athens, Georgia, 30602, USA.; Department of Crop & Soil Sciences, University of Georgia, Athens, Georgia, 30602, USA., Lichman BR; Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, UK. |
| المصدر: | The Plant journal : for cell and molecular biology [Plant J] 2024 Jun; Vol. 118 (5), pp. 1589-1602. Date of Electronic Publication: 2024 Mar 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology Country of Publication: England NLM ID: 9207397 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-313X (Electronic) Linking ISSN: 09607412 NLM ISO Abbreviation: Plant J Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford : Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology, c1991- |
| مواضيع طبية MeSH: | Iridoids*/metabolism, Biosynthetic Pathways/genetics ; Phylogeny ; Genome, Plant/genetics ; Genomics ; Animals ; Cyclopentane Monoterpenes/metabolism ; Pyrones |
| مستخلص: | Iridoids are non-canonical monoterpenoids produced by both insects and plants. An example is the cat-attracting and insect-repelling volatile iridoid nepetalactone, produced by Nepeta sp. (catmint) and aphids. Recently, both nepetalactone biosynthetic pathways were elucidated, showing a remarkable convergent evolution. The iridoid, dolichodial, produced by Teucrium marum (cat thyme) and multiple insect species, has highly similar properties to nepetalactone but its biosynthetic origin remains unknown. We set out to determine the genomic, enzymatic, and evolutionary basis of iridoid biosynthesis in T. marum. First, we generated a de novo chromosome-scale genome assembly for T. marum using Oxford Nanopore Technologies long reads and proximity-by-ligation Hi-C reads. The 610.3 Mb assembly spans 15 pseudomolecules with a 32.9 Mb N50 scaffold size. This enabled identification of iridoid biosynthetic genes, whose roles were verified via activity assays. Phylogenomic analysis revealed that the evolutionary history of T. marum iridoid synthase, the iridoid scaffold-forming enzyme, is not orthologous to typical iridoid synthases but is derived from its conserved paralog. We discovered an enzymatic route from nepetalactol to diverse iridoids through the coupled activity of an iridoid oxidase cytochrome P450 and acetyltransferases, via an inferred acylated intermediate. This work provides a genomic resource for specialized metabolite research in mints and demonstration of the role of acetylation in T. marum iridoid diversity. This work will enable future biocatalytic or biosynthetic production of potent insect repellents, as well as comparative studies into iridoid biosynthesis in insects. (© 2024 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.) |
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| معلومات مُعتمدة: | Georgia Seed Development; MR/S01862X/1 UK Research and Innovation; The Republic of Türkiye (Turkey) Ministry of National Education; Georgia Research Alliance; University of Georgia; BB/V006452/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; Michigan State University |
| فهرسة مساهمة: | Keywords: Teucrium marum; biosynthesis; comparative genomics; enzymology; iridoids; mint family |
| المشرفين على المادة: | 0 (Iridoids) 21651-62-7 (nepetalactone) 0 (Cyclopentane Monoterpenes) 0 (Pyrones) |
| تواريخ الأحداث: | Date Created: 20240315 Date Completed: 20240530 Latest Revision: 20240530 |
| رمز التحديث: | 20240530 |
| DOI: | 10.1111/tpj.16698 |
| PMID: | 38489316 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1365-313X |
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| DOI: | 10.1111/tpj.16698 |