دورية أكاديمية
Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy.
| العنوان: | Protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of neurodevelopmental encephalopathy. |
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| المؤلفون: | Dos Santos AB; Department of Neuroscience, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark. Electronic address: altair.santos@sund.ku.dk., Larsen SD; Department of Neuroscience, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark., Gomez CD; Department of Neuroscience, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark., Sørensen JB; Department of Neuroscience, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark., Perrier JF; Department of Neuroscience, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark. Electronic address: perrier@sund.ku.dk. |
| المصدر: | STAR protocols [STAR Protoc] 2024 Jun 21; Vol. 5 (2), pp. 102954. Date of Electronic Publication: 2024 Mar 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Pyramidal Cells* , Disease Models, Animal*, Animals ; Mice ; Neurodevelopmental Disorders/physiopathology |
| مستخلص: | Here, we present a protocol for quantifying pyramidal neuron hyperexcitability in a mouse model of STXBP1 neurodevelopmental encephalopathy (Stxbp1 hap ). We describe steps for preparing brain slices, positioning electrodes, and performing an excitability test to investigate microcircuit failures. This protocol is based on recording layer 2/3 cortical pyramidal neurons in response to stimulation of two independent sets of excitatory axons that recruit feedforward inhibition microcircuits. For complete details on the use and execution of this protocol, please refer to Dos Santos et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Genetics; Health Sciences; Neuroscience |
| تواريخ الأحداث: | Date Created: 20240316 Date Completed: 20240625 Latest Revision: 20240625 |
| رمز التحديث: | 20240625 |
| مُعرف محوري في PubMed: | PMC10959716 |
| DOI: | 10.1016/j.xpro.2024.102954 |
| PMID: | 38492227 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
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| DOI: | 10.1016/j.xpro.2024.102954 |