دورية أكاديمية
أعرض في EDS

HLA-G isoforms, HLA-C allotype and their expressions differ between early abortus and placenta in relation to spontaneous abortions.

التفاصيل البيبلوغرافية
العنوان: HLA-G isoforms, HLA-C allotype and their expressions differ between early abortus and placenta in relation to spontaneous abortions.
المؤلفون: Bora M; Department of Molecular Biology and Biotechnology Tezpur University, Napaam, Sonitpur, Assam, 784028, India. Electronic address: mayuribora72@gmail.com., Singha S; Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, 784028, Assam, India. Electronic address: sushmitaheisnam95@gmail.com., Madan T; National Institute for Research in Reproductive and Child Health, Department of Innate Immunity, Mumbai, 400012, India. Electronic address: taruna_m@hotmail.com., Deka G; Tezpur Medical College and Hospital, Bihaguri, Tezpur, 784010, Assam, India. Electronic address: gitanjali.dk@gmail.com., Hazarika SG; Gogoi Nursing Home Complex, Tezpur, Assam, 784001, India. Electronic address: drsumitahazarika@gmail.com., Baruah S; Department of Molecular Biology and Biotechnology Tezpur University, Napaam, Sonitpur, Assam, 784028, India. Electronic address: sashibaruah@gmail.com.
المصدر: Placenta [Placenta] 2024 Apr; Vol. 149, pp. 44-53. Date of Electronic Publication: 2024 Mar 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8006349 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-3102 (Electronic) Linking ISSN: 01434004 NLM ISO Abbreviation: Placenta Subsets: MEDLINE
أسماء مطبوعة: Publication: <2010- > : Amsterdam : Elsevier
Original Publication: London W.B. Saunders.
مواضيع طبية MeSH: Abortion, Spontaneous*/genetics , Abortion, Spontaneous*/metabolism , HLA-C Antigens*/genetics , HLA-G Antigens*/genetics , HLA-G Antigens*/metabolism, Female ; Humans ; Infant ; Infant, Newborn ; Pregnancy ; Decidua/metabolism ; DNA Copy Number Variations ; Killer Cells, Natural ; Placenta/metabolism ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; Trophoblasts/metabolism
مستخلص: Introduction: Spontaneous abortion (SAB) affects approximately 10% of clinically recognized pregnancies. Fetal trophobalst invasion and remodeling of maternal spiral arteries is reported to be dependent on crosstalk between HLA-C/HLA-G expressed on extra villous trophoblast (EVTs)and Killer cell Immunoglobin like receptors (KIRs) of decidual NK (dNK). Immune dysfunction in decidua contributes to early miscarriage.
Methodology: The study used mother neonate paired cord blood and term placenta samples (n = 46), elective abortus (n = 17,gestational age = 10-12 weeks of pregnancy) and SAB abortus (n = 24, gestational age = 12-15 weeks of pregnancy) for HLA-G, KIR2D and HLA-C. In addition, term placenta was collected from women with history of spontaneous pregnancy loss (n = 24) and women with history of live birth (n = 32). SSP-PCR was used for genotyping, RT-PCR for gene expression, copy number variation (CNVs) and HLA-C allotyping and ELISA for protein expression studies.
Results: Membrane bound HLA-G4 isoform proportion was higher 39.28%, p = 0.02) in term placenta. SAB abortus had higher proportion of HLA-G3 (50%),while elective abortus exhibited higher proportion of soluble isoforms (HLA-G5, = 5.9, HLA-G6 = 5.9%, HLA-G7 = 11.8%). Higher inhibitory KIR2DL1 content and copy numbers with lower HLA-C2 in SAB contrasted with higher copy numbers of KIR2DS1(p = 0.001), KIR2DS1 + /2DL1 + - HLA-C2 combined genotype in healthy placenta. Elevated KIR2D protein levels (p = 0.001), and concurrently, HLA-C levels were upregulated in healthy placenta.
Conclusion: Our data supports lower cognate receptor ligand KIR2DS1+/2DL1+ HLA-C2 together with predominance of HLA-G3 isoform in SAB as confounding factors in spontaneous pregnancy loss. HLA-G isoforms and expression differed between first trimester abortus and term placenta suggesting temporal modulation and marks novelty of the study.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: CNVs; Elective abortus; HLA-G isoforms; KIR,HLA-C combined genotype; SAB abortus; Spontaneous pregnancy loss
المشرفين على المادة: 0 (HLA-C Antigens)
0 (HLA-G Antigens)
0 (Protein Isoforms)
تواريخ الأحداث: Date Created: 20240316 Date Completed: 20240408 Latest Revision: 20240411
رمز التحديث: 20240412
DOI: 10.1016/j.placenta.2024.02.009
PMID: 38492472
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-3102
DOI:10.1016/j.placenta.2024.02.009