دورية أكاديمية
Chronic dietary iron overload affects hepatic iron metabolism and cognitive behavior in Wistar rats.
| العنوان: | Chronic dietary iron overload affects hepatic iron metabolism and cognitive behavior in Wistar rats. |
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| المؤلفون: | Shete PA; Bioprospecting Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, Maharashtra 411004, India; Savitribai Phule Pune University, Ganeshkhind, Pune, Maharashtra 411007, India. Electronic address: padmajashete@aripune.org., Ghatpande NS; Bioprospecting Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, Maharashtra 411004, India. Electronic address: ghatpandeniraj@gmail.com., Varma ME; Bioprospecting Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, Maharashtra 411004, India. Electronic address: mokshada@chalmers.se., Joshi PV; Bioprospecting Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, Maharashtra 411004, India. Electronic address: joshi.pranav971@gmail.com., Suryavanshi KR; Bioprospecting Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, Maharashtra 411004, India; Savitribai Phule Pune University, Ganeshkhind, Pune, Maharashtra 411007, India. Electronic address: komalsuryavanshi@aripune.org., Misar AV; Bioprospecting Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, Maharashtra 411004, India. Electronic address: avmisar@aripune.org., Jadhav SH; Nanobioscience Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, Maharashtra 411004, India. Electronic address: shjadhav@aripune.org., Apte PP; Bioprospecting Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, Maharashtra 411004, India. Electronic address: pritiapte@aripune.org., Kulkarni PP; Bioprospecting Group, Agharkar Research Institute, G. G. Agarkar Road, Pune, Maharashtra 411004, India; Savitribai Phule Pune University, Ganeshkhind, Pune, Maharashtra 411007, India. Electronic address: ppkulkarni@aripune.org. |
| المصدر: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2024 Jul; Vol. 84, pp. 127422. Date of Electronic Publication: 2024 Mar 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Gustav Fisher Country of Publication: Germany NLM ID: 9508274 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-3252 (Electronic) Linking ISSN: 0946672X NLM ISO Abbreviation: J Trace Elem Med Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Stuttgart : Gustav Fisher Original Publication: Stuttgart ; New York : Gustav Fischer, c1995- |
| مواضيع طبية MeSH: | Rats, Wistar* , Liver*/metabolism , Liver*/drug effects , Iron Overload*/metabolism , Iron*/metabolism, Animals ; Rats ; Male ; Cognition/drug effects ; Brain/metabolism ; Brain/drug effects ; Iron, Dietary/administration & dosage ; Iron, Dietary/pharmacology |
| مستخلص: | Background: Iron accumulation in organs affects iron metabolism, leading to deleterious effects on the body. Previously, it was studied that high dietary iron in various forms and concentrations influences iron metabolism, resulting in iron accumulation in the liver and spleen and cognitive impairment. However, the actual mechanism and impact of long-term exposure to high dietary iron remain unknown. As a result, we postulated that iron overload caused by chronic exposure to excessive dietary iron supplementation would play a role in iron dyshomeostasis and inflammation in the liver and brain of Wistar rats. Methods: Animals were segregated into control, low iron (FAC-Ferric Ammonium Citrate 5000 ppm), and high iron dose group (FAC 20,000 ppm). The outcome of dietary iron overload on Wistar rats was evaluated in terms of body weight, biochemical markers, histological examination of liver and brain tissue, and cognitive-behavioral studies. Also, gene expression of rat brain tissue involving iron transporters Dmt1, TfR1, iron storage protein Fpn1, inflammatory markers Nf-kB, Tnf-α, Il-6, and hepcidin was performed. Results: Our data indicate that excess iron supplementation for 30 weeks leads to decreased body weight, increased serum iron levels, and decreased RBC levels in iron fed Wistar rats. Morris water maze (MWM) studies after 30 weeks showed increased escape latency in the high iron dose group compared with the control group. Histological studies of the high iron dose group showed an iron accumulation in the liver and brain loss of cellular architecture, and cellular degeneration was observed. Excess iron treatment showed upregulation of the Dmt1 gene in iron metabolism and a remarkable increase in the Nf-kB gene in rat brain tissue. Conclusion: The results show chronic excess iron supplementation leads to iron accumulation in the liver, leading to inflammation in Wistar rats. Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest. (Copyright © 2024 Elsevier GmbH. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Gene expression; Inflammation; Iron overload; Iron transporters; Morris Water Maze |
| المشرفين على المادة: | E1UOL152H7 (Iron) 0 (Iron, Dietary) |
| تواريخ الأحداث: | Date Created: 20240316 Date Completed: 20240525 Latest Revision: 20240525 |
| رمز التحديث: | 20240526 |
| DOI: | 10.1016/j.jtemb.2024.127422 |
| PMID: | 38492476 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-3252 |
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| DOI: | 10.1016/j.jtemb.2024.127422 |