دورية أكاديمية
أعرض في EDS

A matter of differentiation: equine enteroids as a model for the in vivo intestinal epithelium.

التفاصيل البيبلوغرافية
العنوان: A matter of differentiation: equine enteroids as a model for the in vivo intestinal epithelium.
المؤلفون: Windhaber C; Institute of Physiology, Pathophysiology and Biophysics, University of Veterinary Medicine, Vienna, Austria., Heckl A; Institute of Physiology, Pathophysiology and Biophysics, University of Veterinary Medicine, Vienna, Austria., Csukovich G; Division of Small Animal Internal Medicine, University of Veterinary Medicine, Vienna, Austria., Pratscher B; Division of Small Animal Internal Medicine, University of Veterinary Medicine, Vienna, Austria., Burgener IA; Division of Small Animal Internal Medicine, University of Veterinary Medicine, Vienna, Austria., Biermann N; Clinical Unit of Equine Surgery, University of Veterinary Medicine, Vienna, Austria., Dengler F; Institute of Physiology, Pathophysiology and Biophysics, University of Veterinary Medicine, Vienna, Austria. franziska.dengler@vetmeduni.ac.at.
المصدر: Veterinary research [Vet Res] 2024 Mar 16; Vol. 55 (1), pp. 30. Date of Electronic Publication: 2024 Mar 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 9309551 Publication Model: Electronic Cited Medium: Internet ISSN: 1297-9716 (Electronic) Linking ISSN: 09284249 NLM ISO Abbreviation: Vet Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 2011- : London : BioMed Central
Original Publication: Paris : Editions Scientifiques Elsevier ; INRA, c1993-
مواضيع طبية MeSH: Gastrointestinal Diseases*/veterinary , Horse Diseases*, Animals ; Horses ; Intestinal Mucosa ; Intestines ; Cell Differentiation ; RNA, Messenger
مستخلص: Epithelial damage due to gastrointestinal disorders frequently causes severe disease in horses. To study the underlying pathophysiological processes, we aimed to establish equine jejunum and colon enteroids (eqJE, eqCE) mimicking the in vivo epithelium. Therefore, enteroids were cultivated in four different media for differentiation and subsequently characterized histomorphologically, on mRNA and on protein level in comparison to the native epithelium of the same donor horses to identify ideal culture conditions for an in vitro model system. With increasing enterocyte differentiation, the enteroids showed a reduced growth rate as well as a predominantly spherical morphology and less budding compared to enteroids in proliferation medium. Combined or individual withdrawal of stem cell niche pathway components resulted in lower mRNA expression levels of stem cell markers and concomitant differentiation of enterocytes, goblet cells and enteroendocrine cells. For eqCE, withdrawal of Wnt alone was sufficient for the generation of differentiated enterocytes with a close resemblance to the in vivo epithelium. Combined removal of Wnt, R-spondin and Noggin and the addition of DAPT stimulated differentiation of eqJE at a similar level as the in vivo epithelium, particularly with regard to enterocytes. In summary, we successfully defined a medium composition that promotes the formation of eqJE and eqCE consisting of multiple cell types and resembling the in vivo epithelium. Our findings emphasize the importance of adapting culture conditions to the respective species and the intestinal segment. This in vitro model will be used to investigate the pathological mechanisms underlying equine gastrointestinal disorders in future studies.
(© 2024. The Author(s).)
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فهرسة مساهمة: Keywords: Organoid; colon; colonoid; differentiation; enterocytes; horse; jejunum; stem cells; three-dimensional cell culture
المشرفين على المادة: 0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20240317 Date Completed: 20240318 Latest Revision: 20240319
رمز التحديث: 20240319
مُعرف محوري في PubMed: PMC10943904
DOI: 10.1186/s13567-024-01283-0
PMID: 38493107
قاعدة البيانات: MEDLINE
الوصف
تدمد:1297-9716
DOI:10.1186/s13567-024-01283-0