دورية أكاديمية
A matter of differentiation: equine enteroids as a model for the in vivo intestinal epithelium.
العنوان: | A matter of differentiation: equine enteroids as a model for the in vivo intestinal epithelium. |
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المؤلفون: | Windhaber C; Institute of Physiology, Pathophysiology and Biophysics, University of Veterinary Medicine, Vienna, Austria., Heckl A; Institute of Physiology, Pathophysiology and Biophysics, University of Veterinary Medicine, Vienna, Austria., Csukovich G; Division of Small Animal Internal Medicine, University of Veterinary Medicine, Vienna, Austria., Pratscher B; Division of Small Animal Internal Medicine, University of Veterinary Medicine, Vienna, Austria., Burgener IA; Division of Small Animal Internal Medicine, University of Veterinary Medicine, Vienna, Austria., Biermann N; Clinical Unit of Equine Surgery, University of Veterinary Medicine, Vienna, Austria., Dengler F; Institute of Physiology, Pathophysiology and Biophysics, University of Veterinary Medicine, Vienna, Austria. franziska.dengler@vetmeduni.ac.at. |
المصدر: | Veterinary research [Vet Res] 2024 Mar 16; Vol. 55 (1), pp. 30. Date of Electronic Publication: 2024 Mar 16. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 9309551 Publication Model: Electronic Cited Medium: Internet ISSN: 1297-9716 (Electronic) Linking ISSN: 09284249 NLM ISO Abbreviation: Vet Res Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2011- : London : BioMed Central Original Publication: Paris : Editions Scientifiques Elsevier ; INRA, c1993- |
مواضيع طبية MeSH: | Gastrointestinal Diseases*/veterinary , Horse Diseases*, Animals ; Horses ; Intestinal Mucosa ; Intestines ; Cell Differentiation ; RNA, Messenger |
مستخلص: | Epithelial damage due to gastrointestinal disorders frequently causes severe disease in horses. To study the underlying pathophysiological processes, we aimed to establish equine jejunum and colon enteroids (eqJE, eqCE) mimicking the in vivo epithelium. Therefore, enteroids were cultivated in four different media for differentiation and subsequently characterized histomorphologically, on mRNA and on protein level in comparison to the native epithelium of the same donor horses to identify ideal culture conditions for an in vitro model system. With increasing enterocyte differentiation, the enteroids showed a reduced growth rate as well as a predominantly spherical morphology and less budding compared to enteroids in proliferation medium. Combined or individual withdrawal of stem cell niche pathway components resulted in lower mRNA expression levels of stem cell markers and concomitant differentiation of enterocytes, goblet cells and enteroendocrine cells. For eqCE, withdrawal of Wnt alone was sufficient for the generation of differentiated enterocytes with a close resemblance to the in vivo epithelium. Combined removal of Wnt, R-spondin and Noggin and the addition of DAPT stimulated differentiation of eqJE at a similar level as the in vivo epithelium, particularly with regard to enterocytes. In summary, we successfully defined a medium composition that promotes the formation of eqJE and eqCE consisting of multiple cell types and resembling the in vivo epithelium. Our findings emphasize the importance of adapting culture conditions to the respective species and the intestinal segment. This in vitro model will be used to investigate the pathological mechanisms underlying equine gastrointestinal disorders in future studies. (© 2024. The Author(s).) |
References: | Nat Protoc. 2013 Dec;8(12):2471-82. (PMID: 24232249) PLoS One. 2021 Aug 23;16(8):e0256143. (PMID: 34424915) Gastroenterology. 2009 Jul;137(1):15-7. (PMID: 19450592) Cell Biol Toxicol. 2005 Jan;21(1):1-26. (PMID: 15868485) BMC Biol. 2019 Apr 11;17(1):33. (PMID: 30975131) Nat Med. 2011 Sep 04;17(10):1225-7. (PMID: 21892181) Annu Rev Anim Biosci. 2018 Feb 15;6:157-175. (PMID: 29144770) Annu Rev Physiol. 2013;75:289-311. (PMID: 23398152) Clin Chem. 2009 Apr;55(4):611-22. (PMID: 19246619) Nature. 2009 May 14;459(7244):262-5. (PMID: 19329995) Vet Res. 2021 Aug 14;52(1):108. (PMID: 34391473) Front Immunol. 2021 Feb 12;11:547102. (PMID: 33643277) In Vitro Cell Dev Biol Anim. 2004 Sep-Oct;40(8-9):278-84. (PMID: 15723563) Stem Cell Res. 2020 Oct;48:101980. (PMID: 32920507) Vet Res. 2018 Jul 3;49(1):54. (PMID: 29970174) Gastroenterology. 2011 Nov;141(5):1762-72. (PMID: 21889923) Equine Vet J. 2018 Mar;50(2):241-248. (PMID: 28792626) Stem Cell Reports. 2022 May 10;17(5):1138-1153. (PMID: 35395175) Dev Cell. 2021 Feb 8;56(3):356-365.e9. (PMID: 33484640) BMC Vet Res. 2022 Dec 23;18(1):445. (PMID: 36564773) Cell Mol Gastroenterol Hepatol. 2017 Nov 03;5(2):113-130. (PMID: 29693040) Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) Nutrients. 2023 Jan 12;15(2):. (PMID: 36678267) Viruses. 2018 Apr 26;10(5):. (PMID: 29701691) Front Cell Dev Biol. 2020 Jul 02;8:375. (PMID: 32714922) Prev Vet Med. 2011 Sep 1;101(3-4):204-18. (PMID: 21733586) Front Vet Sci. 2022 Sep 08;9:947482. (PMID: 36157182) Biol Open. 2017 May 15;6(5):698-705. (PMID: 28347989) Am J Vet Res. 2015 Apr;76(4):358-66. (PMID: 25815577) Annu Rev Physiol. 2009;71:241-60. (PMID: 18808327) Exp Cell Res. 2005 Jun 10;306(2):357-63. (PMID: 15925592) Bio Protoc. 2016 Feb 20;6(4):. (PMID: 27570799) J Vis Exp. 2022 Mar 2;(181):. (PMID: 35311824) PLoS One. 2013 Jun 28;8(6):e66465. (PMID: 23840480) Am J Physiol Cell Physiol. 2021 May 1;320(5):C794-C805. (PMID: 33760661) Am J Physiol Gastrointest Liver Physiol. 2005 Apr;288(4):G705-17. (PMID: 15550553) Biochim Biophys Acta Mol Cell Res. 2017 Jul;1864(7):1183-1194. (PMID: 28322932) United European Gastroenterol J. 2017 Dec;5(8):1073-1081. (PMID: 29238585) J Cell Biol. 1979 Feb;80(2):248-65. (PMID: 88453) Front Vet Sci. 2022 Jul 04;9:921160. (PMID: 35859809) Commun Biol. 2021 Mar 19;4(1):377. (PMID: 33742093) Cell Stem Cell. 2017 Feb 2;20(2):177-190.e4. (PMID: 27939219) Nature. 2007 Oct 25;449(7165):1003-7. (PMID: 17934449) Nature. 2011 Jan 20;469(7330):415-8. (PMID: 21113151) Funct Integr Genomics. 2023 Apr 15;23(2):125. (PMID: 37060478) Nat Commun. 2022 Jan 11;13(1):261. (PMID: 35017529) Equine Vet J. 1997 Nov;29(6):448-53. (PMID: 9413717) Sci Rep. 2020 Feb 26;10(1):3535. (PMID: 32103108) J Biol Chem. 2016 Feb 19;291(8):3759-66. (PMID: 26677228) Cell Death Dis. 2021 Jan 18;12(1):95. (PMID: 33462215) Science. 2013 Jun 7;340(6137):1190-4. (PMID: 23744940) J Vet Intern Med. 2003 May-Jun;17(3):343-8. (PMID: 12774977) Oncotarget. 2017 Dec 26;9(5):6356-6368. (PMID: 29464078) Altern Lab Anim. 2020 Sep-Nov;48(5-6):215-233. (PMID: 33337913) Biochem Biophys Res Commun. 2022 Dec 31;637:153-160. (PMID: 36402064) Curr Gastroenterol Rep. 2010 Oct;12(5):340-8. (PMID: 20683682) Biomaterials. 2019 Feb;194:195-214. (PMID: 30612006) Cells. 2020 Mar 28;9(4):. (PMID: 32231153) Curr Protoc Immunol. 2020 Sep;130(1):e106. (PMID: 32940424) Cell Discov. 2018 Sep 4;4:49. (PMID: 30181900) EMBO Rep. 2023 Sep 6;24(9):e56454. (PMID: 37493498) Vet Res. 2022 Mar 2;53(1):15. (PMID: 35236416) World J Gastroenterol. 2016 Mar 7;22(9):2760-70. (PMID: 26973414) Cell Mol Gastroenterol Hepatol. 2017 Mar 23;4(1):33-46. (PMID: 28560287) Nature. 2005 Jun 16;435(7044):959-63. (PMID: 15959515) BMC Biol. 2018 Feb 01;16(1):19. (PMID: 29391007) Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14700-5. (PMID: 17804805) Cell Tissue Res. 2016 Jul;365(1):123-34. (PMID: 26928041) Gut. 2015 Jun;64(6):911-20. (PMID: 25007816) Stem Cells Int. 2016;2016:3710836. (PMID: 26697073) Gut. 2014 Apr;63(4):610-21. (PMID: 23831735) Gastroenterology. 2007 Sep;133(3):887-96. (PMID: 17678919) Toxicol In Vitro. 2019 Dec;61:104606. (PMID: 31344400) |
فهرسة مساهمة: | Keywords: Organoid; colon; colonoid; differentiation; enterocytes; horse; jejunum; stem cells; three-dimensional cell culture |
المشرفين على المادة: | 0 (RNA, Messenger) |
تواريخ الأحداث: | Date Created: 20240317 Date Completed: 20240318 Latest Revision: 20240319 |
رمز التحديث: | 20240319 |
مُعرف محوري في PubMed: | PMC10943904 |
DOI: | 10.1186/s13567-024-01283-0 |
PMID: | 38493107 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1297-9716 |
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DOI: | 10.1186/s13567-024-01283-0 |