دورية أكاديمية
أعرض في EDS

Arp2/3 complex- and formin-mediated actin cytoskeleton networks facilitate actin binding protein sorting in fission yeast.

التفاصيل البيبلوغرافية
العنوان: Arp2/3 complex- and formin-mediated actin cytoskeleton networks facilitate actin binding protein sorting in fission yeast.
المؤلفون: Homa KE; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, United States., Hocky GM; Department of Chemistry, New York University, New York, NY, United States., Suarez C; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, United States., Kovar DR; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, United States; Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL, United States. Electronic address: drkovar@uchicago.edu.
المصدر: European journal of cell biology [Eur J Cell Biol] 2024 Jun; Vol. 103 (2), pp. 151404. Date of Electronic Publication: 2024 Mar 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Germany NLM ID: 7906240 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-1298 (Electronic) Linking ISSN: 01719335 NLM ISO Abbreviation: Eur J Cell Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: Jena, Germany : Elsevier
Original Publication: Stuttgart : Wissenschaftliche Verlagsgesellschaft, <1979-1997>
مواضيع طبية MeSH: Schizosaccharomyces*/metabolism , Schizosaccharomyces pombe Proteins*/metabolism , Schizosaccharomyces pombe Proteins*/genetics , Actin-Related Protein 2-3 Complex*/metabolism , Actin Cytoskeleton*/metabolism , Microfilament Proteins*/metabolism , Microfilament Proteins*/genetics, Cell Cycle Proteins/metabolism ; Cell Cycle Proteins/genetics ; Actins/metabolism ; Protein Transport ; Cytoskeletal Proteins ; Membrane Glycoproteins
مستخلص: While it is well-established that F-actin networks with specific organizations and dynamics are tightly regulated by distinct sets of associated actin-binding proteins (ABPs), how ABPs self-sort to particular F-actin networks remains largely unclear. We report that actin assembly factors Arp2/3 complex and formin Cdc12 tune the association of ABPs fimbrin Fim1 and tropomyosin Cdc8 to different F-actin networks in fission yeast. Genetic and pharmacological disruption of F-actin networks revealed that Fim1 is preferentially directed to Arp2/3-complex mediated actin patches, whereas Cdc8 is preferentially targeted to formin Cdc12-mediated filaments in the contractile ring. To investigate the role of Arp2/3 complex- and formin Cdc12-mediated actin assembly, we used four-color TIRF microscopy to observe the in vitro reconstitution of ABP sorting with purified proteins. Fim1 or Cdc8 alone bind similarly well to filaments assembled by either assembly factor. However, in 'competition' reactions containing both actin assembly factors and both ABPs, ∼2.0-fold more Fim1 and ∼3.5-fold more Cdc8 accumulates on Arp2/3 complex branch points and formin Cdc12-assembled actin filaments, respectively. These findings indicate that F-actin assembly factors Arp2/3 complex and formin Cdc12 help facilitate the recruitment of specific ABPs, thereby tuning ABP sorting and subsequently establishing the identity of F-actin networks in fission yeast.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2024 The Authors. Published by Elsevier GmbH.. All rights reserved.)
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معلومات مُعتمدة: R01 GM071832 United States GM NIGMS NIH HHS; R01 GM079265 United States GM NIGMS NIH HHS; R35 GM138312 United States GM NIGMS NIH HHS; T32 GM007183 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: Actin cytoskeleton; Actin-binding proteins; Arp2/3 complex; Fimbrin; Formin; Tropomyosin
المشرفين على المادة: 0 (Schizosaccharomyces pombe Proteins)
0 (Actin-Related Protein 2-3 Complex)
0 (Microfilament Proteins)
0 (Cdc12 protein, S pombe)
0 (Cell Cycle Proteins)
0 (Cdc8 protein, S pombe)
0 (Actins)
0 (plastin)
0 (Cytoskeletal Proteins)
0 (Membrane Glycoproteins)
تواريخ الأحداث: Date Created: 20240317 Date Completed: 20240627 Latest Revision: 20240702
رمز التحديث: 20240703
مُعرف محوري في PubMed: PMC11211059
DOI: 10.1016/j.ejcb.2024.151404
PMID: 38493594
قاعدة البيانات: MEDLINE
الوصف
تدمد:1618-1298
DOI:10.1016/j.ejcb.2024.151404