دورية أكاديمية
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A function of spalt major as a sequence-specific DNA binding transcription factor mediates repression of knirps in the Drosophila wing imaginal disc.

التفاصيل البيبلوغرافية
العنوان: A function of spalt major as a sequence-specific DNA binding transcription factor mediates repression of knirps in the Drosophila wing imaginal disc.
المؤلفون: Ostalé CM; Centro de Biología Molecular 'Severo Ochoa', CSIC and Universidad Autónoma de Madrid, Madrid, 28049, Spain., Prado AD; Centro de Biología Molecular 'Severo Ochoa', CSIC and Universidad Autónoma de Madrid, Madrid, 28049, Spain., Martín M; Centro de Biología Molecular 'Severo Ochoa', CSIC and Universidad Autónoma de Madrid, Madrid, 28049, Spain., Esteban N; Centro de Biología Molecular 'Severo Ochoa', CSIC and Universidad Autónoma de Madrid, Madrid, 28049, Spain., López-Varea A; Centro de Biología Molecular 'Severo Ochoa', CSIC and Universidad Autónoma de Madrid, Madrid, 28049, Spain., de Celis JF; Centro de Biología Molecular 'Severo Ochoa', CSIC and Universidad Autónoma de Madrid, Madrid, 28049, Spain. Electronic address: jfdecelis@cbm.csic.es.
المصدر: Developmental biology [Dev Biol] 2024 Jun; Vol. 510, pp. 40-49. Date of Electronic Publication: 2024 Mar 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0372762 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-564X (Electronic) Linking ISSN: 00121606 NLM ISO Abbreviation: Dev Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: San Diego, CA : Elsevier
Original Publication: New York.
مواضيع طبية MeSH: Drosophila*/metabolism , Drosophila Proteins*/genetics , Drosophila Proteins*/metabolism, Animals ; Imaginal Discs/metabolism ; Repressor Proteins/metabolism ; Gene Expression Regulation, Developmental/genetics ; Transcription Factors/metabolism ; Homeodomain Proteins/metabolism ; Wings, Animal
مستخلص: The Spalt transcriptional regulators participate in a variety of cell fate decisions during multicellular development. Vertebrate Spalt proteins have been mostly associated to the organization of heterochromatic regions, but they also contribute regulatory functions through binding to A/T rich motives present in their target genes. The developmental processes in which the Drosophila spalt genes participate are well known through genetic analysis, but the mechanism by which the Spalt proteins regulate transcription are still unknown. Furthermore, despite the prominent changes in gene expression associated to mutations in the spalt genes, the specific DNA sequences they bind are unknow. Here, we analyze a DNA fragment present in the regulatory region of the knirps gene. Spalt proteins are candidate repressors of knirps expression during the formation of the venation pattern in the wing disc, and we identified a minimal conserved 30bp sequence that binds to Spalt major both in vivo and in vitro. This sequence mediates transcriptional repression in the central region of the wing blade, constituting the first confirmed case of a direct regulatory interaction between Spalt major and its target DNA in Drosophila. Interestingly, we also find similar sequences in a set of eight novel candidate Spalt target genes, pointing to a common mechanism of transcriptional repression mediated by Spalt proteins.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Gene expression; Knirps regulation; Spalt proteins; Vein patterning
المشرفين على المادة: 0 (Repressor Proteins)
0 (Drosophila Proteins)
0 (Transcription Factors)
0 (Homeodomain Proteins)
تواريخ الأحداث: Date Created: 20240317 Date Completed: 20240415 Latest Revision: 20240520
رمز التحديث: 20240521
DOI: 10.1016/j.ydbio.2024.03.004
PMID: 38493946
قاعدة البيانات: MEDLINE
الوصف
تدمد:1095-564X
DOI:10.1016/j.ydbio.2024.03.004