دورية أكاديمية
Structural modeling and gene expression analysis of phosvitinless vitellogenin (vgc) in the Indian freshwater murrel, Channa punctatus (Bloch, 1793).
العنوان: | Structural modeling and gene expression analysis of phosvitinless vitellogenin (vgc) in the Indian freshwater murrel, Channa punctatus (Bloch, 1793). |
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المؤلفون: | Vijay P; Department of Zoology, University of Delhi, Delhi 110007, India., Panwar D; Center for Individualized Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA., Narwal R; Department of Zoology, University of Delhi, Delhi 110007, India., Sehgal N; Department of Zoology, University of Delhi, Delhi 110007, India. Electronic address: nsehgal@zoology.du.ac.in. |
المصدر: | General and comparative endocrinology [Gen Comp Endocrinol] 2024 Jun 01; Vol. 352, pp. 114491. Date of Electronic Publication: 2024 Mar 15. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370735 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-6840 (Electronic) Linking ISSN: 00166480 NLM ISO Abbreviation: Gen Comp Endocrinol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: New York, NY : Academic Press Original Publication: New York. |
مواضيع طبية MeSH: | Vitellogenins*/genetics , Vitellogenins*/metabolism , Channa punctatus*, Animals ; Female ; Egg Proteins/genetics ; Gene Expression Profiling ; Fresh Water |
مستخلص: | Vitellogenin (Vg) is a female-specific egg-yolk precursor protein, synthesized in the liver of fish in response to estrogens. In the present study, complete gene of phosvitinless vitellogenin (vgc) was sequenced, its 3D structure was predicted and validated by web-based softwares. The complete nucleotide sequence of vgc was 4126 bp which encodes for 1272 amino acids and showed the presence of three conserved domains viz. LPD_N, DUF1943 and DUF1944. The retrieved amino acid sequence of VgC protein was subjected to in silico analysis for understanding the structural and functional properties of protein. mRNA levels of multiple vg genes have also been quantified during annual reproductive cycle employing qPCR. A correlation has been observed between seasonal changes in gonadosomatic index with estradiol levels and hepatic expression of three types of vg genes (vga, vgb, vgc) during ovarian cycle of murrel. During preparatory phase, when photoperiod and temperature are low; low titre of E Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
فهرسة مساهمة: | Keywords: 3D structure modeling; Bioinformatics; Channa punctatus; Phosvitinless vitellogenin (VgC); Real Time PCR (qPCR) |
المشرفين على المادة: | 0 (Vitellogenins) 0 (Egg Proteins) |
تواريخ الأحداث: | Date Created: 20240317 Date Completed: 20240422 Latest Revision: 20240607 |
رمز التحديث: | 20240607 |
DOI: | 10.1016/j.ygcen.2024.114491 |
PMID: | 38494038 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1095-6840 |
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DOI: | 10.1016/j.ygcen.2024.114491 |